Regulation of endocytosis via the oxygen-sensing pathway.
AuthorsWang Y, Roche O, Yan MS, Finak G, Evans AJ, Metcalf JL, Hast BE, Hanna SC, Wondergem B, Furge KA, Irwin MS, Kim WY, Teh BT, Grinstein S, Park M, Marsden PA, Ohh M,
JournalNat Med
PubMed ID19252501
'Tumor hypoxia is associated with disease progression, resistance to conventional cancer therapies and poor prognosis. Hypoxia, by largely unknown mechanisms, leads to deregulated accumulation of and signaling via receptor tyrosine kinases (RTKs) that are critical for driving oncogenesis. Here, we show that hypoxia or loss of von Hippel-Lindau protein--the principal ... More
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
AuthorsLangelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist WI
JournalJ Virol
PubMed ID16973552
'The budding of many enveloped RNA viruses, including human immunodeficiency virus type 1 (HIV-1), requires some of the same cellular machinery as vesicle formation at the multivesicular body (MVB). In Saccharomyces cerevisiae, the ESCRT-II complex performs a central role in MVB protein sorting and vesicle formation, as it is recruited ... More
Two di-leucine motifs regulate trafficking of mucolipin-1 to lysosomes.
AuthorsVergarajauregui S, Puertollano R
JournalTraffic
PubMed ID16497227
'Mutations in the mucolipin-1 gene have been linked to mucolipidosis type IV, a lysosomal storage disorder characterized by severe neurological and ophthalmologic abnormalities. Mucolipin-1 is a membrane protein containing six putative transmembrane domains with both its N- and C-termini localized facing the cytosol. To gain information on the sorting motifs ... More
Rab14 is part of the early endosomal clathrin-coated TGN microdomain.
AuthorsProikas-Cezanne T, Gaugel A, Frickey T, Nordheim A,
JournalFEBS Lett
PubMed ID16962593
'Rab14 localizes to the Golgi/TGN and to early endosomes, but its biological function remains unclear. By structural modeling, we identified Rab14-specific residues and established a close relationship between the Rab2/Rab4/Rab14, Rab11/25 and Rab39 sub-groups within the Rab protein family. By quantitative confocal microscopy and by density centrifugation we show that ... More
Interactions of TOM1L1 with the multivesicular body sorting machinery.
AuthorsPuertollano R
JournalJ Biol Chem
PubMed ID15611048
'Tom1L1 (Tom1-like1) and related proteins Tom1 (Target of Myb1) and Tom1L2 (Tom1-like2) constitute a new protein family characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. Recently it was demonstrated that the GAT domain of both Tom1 and ... More
Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators.
AuthorsWang J, Xie X,
JournalActa Pharmacol Sin
PubMed ID17883960
'AIM: To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS: Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal growth factor (EGF) was used to visualize the internalized ... More
Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance.
AuthorsNevo J, Mattila E, Pellinen T, Yamamoto DL, Sara H, Iljin K, Kallioniemi O, Bono P, Heikkilä P, Joensuu H, Wärri A, Ivaska J,
JournalClin Cancer Res
PubMed ID19825952
'Only few predictive factors for the clinical activity of anti-epidermal growth factor receptor (EGFR) therapy are available. Mammary-derived growth inhibitor (MDGI) is a small cytosolic protein suggested to play a role in the differentiation of epithelial cells. Here, we have investigated the effect of MDGI expression on the EGFR signaling ... More
The identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases.
AuthorsUngewickell A, Hugge C, Kisseleva M, Chang SC, Zou J, Feng Y, Galyov EE, Wilson M, Majerus PW
JournalProc Natl Acad Sci U S A
PubMed ID16365287
Numerous inositol polyphosphate 5-phosphatases catalyze the degradation of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P(2)) to phosphatidylinositol-4-phosphate (PtdIns-4-P). An alternative pathway to degrade PtdIns-4,5-P(2) is the hydrolysis of PtdIns-4,5-P(2) by a 4-phosphatase, leading to the production of PtdIns-5-P. Whereas the bacterial IpgD enzyme is known to catalyze this reaction, no such mammalian enzyme has been ... More
Dynein is required for receptor sorting and the morphogenesis of early endosomes.
AuthorsDriskell OJ, Mironov A, Allan VJ, Woodman PG
JournalNat Cell Biol
PubMed ID17173037
The early endosome is organised into domains to ensure the separation of cargo. Activated mitogenic receptors, such as epidermal growth factor (EGF) receptor, are concentrated into vacuoles enriched for the small GTPase Rab5, which progressively exclude nutrient receptors, such as transferrin receptor, into neighbouring tubules. These vacuoles become enlarged, increase ... More
Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion.
Authors
JournalAutophagy
PubMed ID29940786
K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.
Authors
JournalCell Rep
PubMed ID35196483
PICALM Rescues Endocytic Defects Caused by the Alzheimer's Disease Risk Factor APOE4.
Authors
JournalCell Rep
PubMed ID33027662
Vacuole membrane protein 1 marks endoplasmic reticulum subdomains enriched in phospholipid synthesizing enzymes and is required for phosphoinositide distribution.
Authors
JournalTraffic
PubMed ID29761602
High-efficacy subcellular micropatterning of proteins using fibrinogen anchors.
Authors
JournalJ Cell Biol
PubMed ID33416860
Endocytic trafficking of GAS6-AXL complexes is associated with sustained AKT activation.
Authors
JournalCell Mol Life Sci
PubMed ID35622156
Ependyma-expressed CCN1 restricts the size of the neural stem cell pool in the adult ventricular-subventricular zone.
Authors
JournalEMBO J
PubMed ID32009252
Beclin 1 Promotes Endosome Recruitment of Hepatocyte Growth Factor Tyrosine Kinase Substrate to Suppress Tumor Proliferation.
Authors
JournalCancer Res
PubMed ID31744816
Biallelic MADD variants cause a phenotypic spectrum ranging from developmental delay to a multisystem disorder.
Authors
JournalBrain
PubMed ID32761064
Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas.