表皮生长因子、生物素化、与 Alexa Fluor™ 647 链霉亲和素(Alexa Fluor™ 647 EGF 复合物)的复合物
表皮生长因子、生物素化、与 Alexa Fluor™ 647 链霉亲和素(Alexa Fluor™ 647 EGF 复合物)的复合物
Invitrogen™

表皮生长因子、生物素化、与 Alexa Fluor™ 647 链霉亲和素(Alexa Fluor™ 647 EGF 复合物)的复合物

我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)了解更多信息
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货号数量
E35351100 μg
货号 E35351
价格(CNY)
7,633.00
Each
添加至购物车
数量:
100 μg
价格(CNY)
7,633.00
Each
添加至购物车
我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)、Oregon Green™ 514 和四甲基罗丹明,使用与 EGF 直接连接的染料构建。EGF 与 Alexa Fluor™ 染料和 Texas Red™ 染料的偶联物是染料–链霉亲和素分子和生物素化 EGF 的复合物。生物素化偶联物利用生物素-XX,它含有一个长间隔臂,旨在增强探针对 EGF 受体的亲和力。

表皮生长因子偶联物规格:
• EGF 分子:53 个氨基酸,MW=6,045 Da
• 标记(激发/发射波长):生物素-XX 与 Alexa Fluor™ 647 链霉素亲和素 (∼650/665 nm) 的复合物
• 每个 EGF 分子上的荧光基团分子数量:∼2–3 个
• 通常使用流式细胞仪、荧光显微镜或荧光计监测荧光信号


查找更多的受体结合和吞噬作用探针
我们提供多种用于研究受体介导的内吞作用的荧光标记探针,用于内吞作用和胞吐作用的膜标记物,以及检测内吞的荧光配体的方法。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
标签或染料Alexa Fluor 染料
产品线Alexa Fluor
数量100 μg
运输条件湿冰
产品类型表皮生长因子
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (16)

引用和文献
Abstract
The HECT domain ligase itch ubiquitinates endophilin and localizes to the trans-Golgi network and endosomal system.
Authors:Angers A, Ramjaun AR, McPherson PS
Journal:J Biol Chem
PubMed ID:14684745
'Endophilin A1 is an SH3 domain-containing protein functioning in membrane trafficking on the endocytic pathway. We have identified the E3 ubiquitin ligase itch/AIP4 as an endophilin A1-binding partner. Itch belongs to the Nedd4/Rsp5p family of proteins and contains an N-terminal C2 domain, four WW domains and a catalytic HECT domain. ... More
Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes.
Authors:Lakadamyali M, Rust MJ, Zhuang X
Journal:Cell
PubMed ID:16530046
'Cells rely on the correct sorting of endocytic ligands and receptors for proper function. Early endosomes have been considered as the initial sorting station where cargos for degradation separate from those for recycling. Using live-cell imaging to monitor individual endosomes and ligand particles in real time, we have discovered a ... More
Simultaneous prospective purification of adult subventricular zone neural stem cells and their progeny.
Authors:Pastrana E, Cheng LC, Doetsch F,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19332781
'The ability to prospectively isolate adult neural stem cells and their progeny is crucial to study their biology and therapeutic potential. Stem cells in adult mammalian neurogenic niches are a subset of astrocytes. A major limitation in the field has been the inability to distinguish stem cell astrocytes from niche ... More
A fluid membrane-based soluble ligand-display system for live-cell assays.
Authors:Nam JM, Nair PM, Neve RM, Gray JW, Groves JT
Journal:Chembiochem
PubMed ID:16456901
Test samples for optimizing STORM super-resolution microscopy.
Authors:Metcalf DJ, Edwards R, Kumarswami N, Knight AE,
Journal:
PubMed ID:24056752
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize ... More