The HECT domain ligase itch ubiquitinates endophilin and localizes to the trans-Golgi network and endosomal system.
AuthorsAngers A, Ramjaun AR, McPherson PS
JournalJ Biol Chem
PubMed ID14684745
'Endophilin A1 is an SH3 domain-containing protein functioning in membrane trafficking on the endocytic pathway. We have identified the E3 ubiquitin ligase itch/AIP4 as an endophilin A1-binding partner. Itch belongs to the Nedd4/Rsp5p family of proteins and contains an N-terminal C2 domain, four WW domains and a catalytic HECT domain. ... More
Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes.
AuthorsLakadamyali M, Rust MJ, Zhuang X
JournalCell
PubMed ID16530046
'Cells rely on the correct sorting of endocytic ligands and receptors for proper function. Early endosomes have been considered as the initial sorting station where cargos for degradation separate from those for recycling. Using live-cell imaging to monitor individual endosomes and ligand particles in real time, we have discovered a ... More
Simultaneous prospective purification of adult subventricular zone neural stem cells and their progeny.
AuthorsPastrana E, Cheng LC, Doetsch F,
JournalProc Natl Acad Sci U S A
PubMed ID19332781
'The ability to prospectively isolate adult neural stem cells and their progeny is crucial to study their biology and therapeutic potential. Stem cells in adult mammalian neurogenic niches are a subset of astrocytes. A major limitation in the field has been the inability to distinguish stem cell astrocytes from niche ... More
A fluid membrane-based soluble ligand-display system for live-cell assays.
AuthorsNam JM, Nair PM, Neve RM, Gray JW, Groves JT
JournalChembiochem
PubMed ID16456901
Test samples for optimizing STORM super-resolution microscopy.
AuthorsMetcalf DJ, Edwards R, Kumarswami N, Knight AE,
Journal
PubMed ID24056752
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize ... More
Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting.
AuthorsFili N, Calleja V, Woscholski R, Parker PJ, Larijani B
JournalProc Natl Acad Sci U S A
PubMed ID17030795
It is increasingly recognized that the compartmental organization of signaling processes has a profound influence on cellular behavior. However, our inability to influence these compartmental events in a spatially restricted and acute manner limits our understanding of causation. To determine whether local compartmental loss of a phosphoinositide disrupts the normal ... More
Sortilin limits EGFR signaling by promoting its internalization in lung cancer.
Authors
JournalNat Commun
PubMed ID29084952
Single-cell analysis reveals T cell infiltration in old neurogenic niches.
Authors
JournalNature
PubMed ID31270459
Single-Cell Profiling and SCOPE-Seq Reveal Lineage Dynamics of Adult Ventricular-Subventricular Zone Neurogenesis and NOTUM as a Key Regulator.
Authors
JournalCell Rep
PubMed ID32579931
Selective inhibitor of endosomal trafficking pathways exploited by multiple toxins and viruses.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID24191014
Protein crowding mediates membrane remodeling in upstream ESCRT-induced formation of intraluminal vesicles.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID33139578
Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.
Authors
JournalNat Commun
PubMed ID30050131
The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7.
Authors
JournalCell Rep
PubMed ID34731620
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Authors
JournalNat Commun
PubMed ID34880248
Visualization of self-delivering hydrophobically modified siRNA cellular internalization.