Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis.
AuthorsEdo Á,Calvo-Barreiro L,Eixarch H,Bosch A,Chillón M,Espejo C
JournalNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
PubMed ID35902536
The pathogenic role of the interleukin 21 (IL-21) in different autoimmune diseases, such as multiple sclerosis (MS), has been extensively studied. However, its pleiotropic nature makes it a cytokine that may exhibit different activity depending on the immunological stage of the disease. In this study, we developed a gene therapy ... More
Glucan Particles Are a Powerful Adjuvant for the HBsAg, Favoring Antiviral Immunity.
AuthorsSoares E, Groothuismink ZMA, Boonstra A, Borges O
JournalMol Pharm
PubMed ID30964694
'The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options. Pathogen-like particles are a promising approach. The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition. This study aimed to evaluate the adjuvant potential of ... More
Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment.
'Monoclonal antibodies specific for foreign antigens, auto-antigens, allogeneic antigens and tumour neo-antigens in the context of major histocompatibility complex II (MHCII) are highly desirable as novel immunotherapeutics. However, there is no standard protocol for the efficient generation of monoclonal antibodies that recognize peptide in the context of MHCII, and only ... More
Inhibition of Ras GTPases prevents Collagen-Induced Arthritis by Reducing the Generation of Pathogenic CD4
AuthorsZayoud M, Vax E, Elad-Sfadia G, Barshack I, Pinkas-Kramarski R, Goldstein I
JournalACR Open Rheumatol
PubMed ID32869536
'RasGTPases are master regulators of multiple intracellular signaling cascades. Perturbation of this pathway has been implicated in the pathogenesis of rheumatoid arthritis (RA). In this study we aimed to define the therapeutic potential of a novel RasGTPases inhibitor, farnesylthiosalicylate (FTS), in the preclinical mouse model of collagen-induced arthritis (CIA) and ... More
Intra-tumoral production of IL18, but not IL12, by TCR-engineered T cells is non-toxic and counteracts immune evasion of solid tumors.
AuthorsKunert A, Chmielewski M, Wijers R, Berrevoets C, Abken H, Debets R
JournalOncoimmunology
PubMed ID29296541
Adoptive therapy with engineered T cells shows promising results in treating patients with malignant disease, but is challenged by incomplete responses and tumor recurrences. Here, we aimed to direct the tumor microenvironment in favor of a successful immune response by local secretion of interleukin (IL-) 12 and IL-18 by sadministered ... More
Memory CD8 T cells mediate severe immunopathology following respiratory syncytial virus infection.
Memory CD8 T cells can provide protection from re-infection by respiratory viruses such as influenza and SARS. However, the relative contribution of memory CD8 T cells in providing protection against respiratory syncytial virus (RSV) infection is currently unclear. To address this knowledge gap, we utilized a prime-boost immunization approach to ... More
Topical Application of the Quaternary Ammonium Compound Didecyldimethylammonium Chloride Activates Type 2 Innate Lymphoid Cells and Initiates a Mixed-Type Allergic Response.
AuthorsShane HL, Lukomska E, Kashon ML, Anderson SE
JournalToxicol Sci
PubMed ID30649503
Didecyldimethylammonium chloride (DDAC) is an antimicrobial dialkyl-quaternary ammonium compound used in industrial and commercial products. Clinical data suggest that DDAC exposure elicits multiple types of hypersensitivity reactions; here, we confirm this observation in a BALB/c murine model. To examine the immunological mechanism behind this mixed-type response and the potential involvement ... More
Topical exposure to triclosan inhibits Th1 immune responses and reduces T cells responding to influenza infection in mice.
AuthorsShane HL, Othumpangat S, Marshall NB, Blachere F, Lukomska E, Weatherly LM, Baur R, Noti JD, Anderson SE
JournalPLoS One
PubMed ID33373420
Healthcare workers concurrently may be at a higher risk of developing respiratory infections and allergic disease, such as asthma, than the general public. Increased incidence of allergic diseases is thought to be caused, in part, due to occupational exposure to chemicals that induce or augment Th2 immune responses. However, whether ... More
Evaluating the Role of STAT3 in CD4
AuthorsGohir W, McTaggart L, Kus JV, Mazzulli T, Kumar D, Humar A, Husain S
JournalInfect Immun
PubMed ID33526558
We aimed to determine whether T cell-specific STAT3 deletion influences the immune response to