Integrin beta1-focal adhesion kinase signaling directs the proliferation of metastatic cancer cells disseminated in the lungs.
AuthorsShibue T, Weinberg RA,
JournalProc Natl Acad Sci U S A
PubMed ID19502425
The development of metastases is an extended and inefficient process involving multiple steps. The last of these involves the growth of micrometastases into macroscopic tumors. We show here that intravenously injected, nonmetastatic cancer cells cease proliferating after extravasating into the parenchyma of the lungs; this response is attributable to the ... More
Microfluidic flow-flash: method for investigating protein dynamics.
AuthorsToepke MW, Brewer SH, Vu DM, Rector KD, Morgan JE, Gennis RB, Kenis PJ, Dyer RB
JournalAnal Chem
PubMed ID17194129
We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design ... More
Utilization of kinetically enhanced monovalent binding affinity by immunoassays based on multivalent nanoparticle-antibody bioconjugates.
AuthorsSoukka T, Härmä H, Paukkunen J, Lövgren T
JournalAnal Chem
PubMed ID11393849
The monovalent binding affinity of high binding site density nanoparticle-antibody bioconjugates is shown to exceed the intrinsic affinity of the original, monoclonal antibody. The nanoparticle-antibody bioconjugates were prepared by covalent coupling of antibodies to long-lifetime fluorescent, europium(III) chelate nanoparticles, 107 nm in diameter. Experiments were carried out in standard microtitration ... More