FM™ 4-64FX,FM™ 4-64 膜染色剂的可固定类似物
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FM™ 4-64FX,FM™ 4-64 膜染色剂的可固定类似物
Invitrogen™

FM™ 4-64FX,FM™ 4-64 膜染色剂的可固定类似物

亲脂性探针 FM™ 4-64FX 在水中发弱荧光,但在与质膜外膜结合后会发出强烈的荧光,使质膜分散染色。此结合过程快速可逆,“FX”表示该类似物可采用醛类固定剂固定。此产品还是一种出色的试剂,可用于鉴定主动应激神经元,研究活性依赖性囊泡循环机制了解更多信息
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货号数量
F3465310 x 100 μg
货号 F34653
价格(CNY)
6,269.00
飞享价
Ends: 31-Dec-2025
8,499.00
共减 2,230.00 (26%)
Each
添加至购物车
数量:
10 x 100 μg
价格(CNY)
6,269.00
飞享价
Ends: 31-Dec-2025
8,499.00
共减 2,230.00 (26%)
Each
添加至购物车
亲脂性探针 FM™ 4-64FX 在水中发弱荧光,但在与质膜外膜结合后会发出强烈的荧光,使质膜分散染色。此结合过程快速可逆,“FX”表示该类似物可采用醛类固定剂固定。此产品还是一种出色的试剂,可用于鉴定主动应激神经元,研究活性依赖性囊泡循环机制。
仅供科研使用。不可用于诊断程序。
规格
颜色红外的
检测方法荧光
适用于(设备)荧光显微镜
产品线FM™
数量10 x 100 μg
运输条件室温
标签类型Fluorescent Dye
产品类型亲脂性探针
亚细胞定位细胞膜
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.