FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain
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FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain
Citations & References (23)
Invitrogen™

FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain

The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of theRead more
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Catalog NumberQuantity
F3465310 x 100 μg
Catalog number F34653
Price (CNY)
6,269.00
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Ends: 31-Dec-2025
8,499.00
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Quantity:
10 x 100 μg
Price (CNY)
6,269.00
Online Exclusive
Ends: 31-Dec-2025
8,499.00
Save 2,230.00 (26%)
Each
Add to cart
The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and 'FX' denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorInfrared
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineFM
Quantity10 x 100 μg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeLipophilic Probe
SubCellular LocalizationPlasma Membrane
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (23)

Citations & References
Abstract
Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.
Authors:Xiong JP, Mahalingham B, Alonso JL, Borrelli LA, Rui X, Anand S, Hyman BT, Rysiok T, Müller-Pompalla D, Goodman SL, Arnaout MA,
Journal:J Cell Biol
PubMed ID:19704023
'We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and ... More
Endocytic trafficking from the small intestinal brush border probed with FM dye.
Authors:Hansen GH, Rasmussen K, Niels-Christiansen LL, Danielsen EM,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:19679822
The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More
A stochastic mechanism for biofilm formation by Mycoplasma pulmonis.
Authors:Simmons WL, Bolland JR, Daubenspeck JM, Dybvig K,
Journal:J Bacteriol
PubMed ID:17142389
Bacterial biofilms are communities of bacteria that are enclosed in an extracellular matrix. Within a biofilm the bacteria are protected from antimicrobials, environmental stresses, and immune responses from the host. Biofilms are often believed to have a highly developed organization that is derived from differential regulation of the genes that ... More
Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.
Authors:Shen W, Wu B, Zhang Z, Dou Y, Rao ZR, Chen YR, Duan S
Journal:Neuron
PubMed ID:16675395
Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in ... More
Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli.
Authors:Ghosh AS, Young KD
Journal:J Bacteriol
PubMed ID:15743937
In bacteria, several physiological processes once thought to be the products of uniformly dispersed reactions are now known to be highly asymmetric, with some exhibiting interesting geometric localizations. In particular, the cell envelope of Escherichia coli displays a form of subcellular differentiation in which peptidoglycan and outer membrane proteins at ... More
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