Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe.
Authors:Ou B, Hampsch-Woodill M, Flanagan J, Deemer EK, Prior RL, Huang D,
Journal:J Agric Food Chem
PubMed ID:11982397
'A novel fluorometric method has been developed to evaluate hydroxyl radical prevention capacity using fluorescein (FL) as the probe. The hydroxyl radical is generated by a Co(II)-mediated Fenton-like reaction, and the hydroxyl radical formation under the experimental condition is indirectly confirmed by the hydroxylation of p-hydroxybenzoic acid. The fluorescence decay ... More