'A novel fluorometric method has been developed to evaluate hydroxyl radical prevention capacity using fluorescein (FL) as the probe. The hydroxyl radical is generated by a Co(II)-mediated Fenton-like reaction, and the hydroxyl radical formation under the experimental condition is indirectly confirmed by the hydroxylation of p-hydroxybenzoic acid. The fluorescence decay ... More
Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region.
AuthorsVelapoldi RA, Tønnesen HH,
JournalJ Fluoresc
PubMed ID15617389
This communication describes the determination of corrected fluorescence emission spectra and fluorescence quantum yields for fluorescein, sulforhodamine 101 and other standard fluorescent dyes.
Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe.
AuthorsOu B, Hampsch-Woodill M, Prior RL,
JournalJ Agric Food Chem
PubMed ID11599998
An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3',6'-dihydroxyspiro[isobenzofuran-1[3H],9'[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin. The oxidized FL products induced by peroxyl radical were identified by LC/MS, and the reaction mechanism was determined ... More
Fluorescein as a model molecular calculator with reset capability.
AuthorsMargulies D, Melman G, Shanzer A,
JournalNat Mater
PubMed ID16155575
The evolution of molecules capable of performing boolean operations has gone a long way since the inception of the first molecular AND logic gate, followed by other logic functions, such as XOR and INHIBIT, and has reached the stage where these tiny processors execute arithmetic calculations. Molecular logic gates that ... More
Programming cell-free biosensors with DNA strand displacement circuits.
Authors
JournalNat Chem Biol
PubMed ID35177837
Absolute Quantification of RNA Molecules Using Fluorescence Correlation Spectroscopy with Certified Reference Materials.
AuthorsSasaki A, Yamamoto J, Kinjo M, Noda N
JournalAnal Chem
PubMed ID30109932
'The accuracy and precision of quantification values of biomolecules, such as nucleic acids, are critical for the reliability of biomedical research and clinical examinations. To obtain an accurate quantitative value, it is necessary to use a measurement standard that has the same sequence and length as the target gene. The ... More
Monomeric IgG is neuroprotective via enhancing microglial recycling endocytosis and TNF-alpha.
Authors
JournalJ Neurosci
PubMed ID19020014
Quantitative Flow Cytometry Measurements in Antibodies Bound per Cell Based on a CD4 Reference.
'Multicolor flow cytometer assays with fluorescently labeled antibodies are routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens either on the cell surface or intracellularly. The cell number and specific receptors/antigens serve as biomarkers for pathological conditions at ... More
Evaluating performance in three-dimensional fluorescence microscopy.
Authors
JournalJ Microsc
PubMed ID18045334
Dynamic Measurement of Nanoflows: Analysis and Theory of an Optofluidic Flowmeter.
Authors
JournalPhys Rev Appl
PubMed ID32166098
Cell-free biosensors for rapid detection of water contaminants.
Authors
JournalNat Biotechnol
PubMed ID32632301
Analysis of individual mitochondria via fluorescent immunolabeling with Anti-TOM22 antibodies.
Authors
JournalAnal Bioanal Chem
PubMed ID24481619
Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids.
AuthorsMaioli M, Basoli V, Santaniello S, Cruciani S, Delitala AP, Pinna R, Milia E, Grillari-Voglauer R, Fontani V, Rinaldi S, Muggironi R, Pigliaru G, Ventura C
JournalStem Cells Int
PubMed ID26880937
Human dental pulp stem cells (hDPSCs) have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A (VEGF A) has been recently reported to act as a major conductor of osteogenesis in ... More
Pulsatile release from a flat self-oscillating chitosan macrogel.
AuthorsAnna I, Katarina N
JournalJ Mater Chem B
PubMed ID32255072
Coupling oscillatory chemical reactions to smart materials which can respond to external stimuli is considered an answer to the long-standing issue of pulsatile drug delivery. Although a number of coupled architectures exist, there are no systems reporting pH-controlled pulsed drug release based on chemical oscillators. In this paper, we report ... More