Arginase II downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells.
AuthorsGotoh T, Mori M
JournalJ Cell Biol
PubMed ID9971738
'Excess nitric oxide (NO) induces apoptosis of some cell types, including macrophages. As NO is synthesized by NO synthase (NOS) from arginine, a common substrate of arginase, these two enzymes compete for arginine. There are two known isoforms of arginase, types I and II. Using murine macrophage-like RAW 264.7 cells, ... More
Augmentation or inhibition of IFN-gamma-induced MHC class II expression by lipopolysaccharides. The roles of TNF-alpha and nitric oxide, and the importance of the sequence of signaling.
AuthorsSicher SC, Chung GW, Vazquez MA, Lu CY
JournalJ Immunol
PubMed ID7499872
'MHC class II expression on macrophages is one determinant of Ag presentation and the vigor of CD4+ T cell immunity. We show that LPS may either inhibit or augment IFN-gamma-induced MHC class II on macrophages depending on the sequence of the IFN-gamma and LPS signals. LPS inhibited MHC class II ... More
Enzymatic microtiter plate-based nitrate detection in environmental and medical analysis.
AuthorsBorcherding H, Leikefeld S, Frey C, Diekmann S, Steinrücke P
JournalAnal Biochem
PubMed ID10860492
'Our microtiter plate assay is based on the enzymatic reduction of nitrate by dissimilatory nitrate reductase from Pseudomonas stutzeri [EC 1.7.99.4]. Exogenous redox mediators like methyl viologen, methylene blue, and cibachron blue were applied to reduce nitrate reductase. Concentrations of 0.02-0.9 mM nitrate can be detected with +/-6% standard deviation, ... More
Measurement of nitrite and nitrate in biological fluids by gas chromatography-mass spectrometry and by the Griess assay: problems with the Griess assay--solutions by gas chromatography-mass spectrometry.
'Assay methods based on the Griess reaction are frequently used to measure nitrite and nitrate in urine, plasma, and other biological fluids. With minor exceptions, careful attention has not been paid in extending the Griess assay from aqueous solutions to biological fluids, In the present study, parallel measurements of nitrite ... More
Pyrimidinoceptor-mediated potentiation of inducible nitric-oxide synthase induction in J774 macrophages. Role of intracellular calcium.
AuthorsChen BC, Chou CF, Lin WW
JournalJ Biol Chem
PubMed ID9792689
'We have shown that, in murine J774 macrophages, binding of UTP to pyrimidinoceptors stimulates phosphoinositide (PI) breakdown and an increase in [Ca2+]i. In this study, UTP modulation of the expression of inducible nitric-oxide synthase (iNOS) was investigated. Although UTP alone had no effect, stimulation of J774 cells with a combination ... More
Dehydroepiandrosterone modulation of lipopolysaccharide-stimulated monocyte cytotoxicity.
AuthorsMcLachlan JA, Serkin CD, Bakouche O
JournalJ Immunol
PubMed ID8598481
'Dehydroepiandrosterone (DHEA), the predominant androgen secreted by the adrenal cortex, can be converted to both potent androgens and estrogens. In addition to its role as a precursor for other steroid hormones, DHEA has been proposed to play an important role in immunity. This study has investigated DHEA modulation of LPS-induced ... More
Carbohydrate oxidation acidifies endosomes, regulating antigen processing and TLR9 signaling.
AuthorsLewis CJ, Cobb BA,
JournalJ Immunol
PubMed ID20200279
'Phagocytes kill encapsulated microbes through oxidative cleavage of surface carbohydrates, releasing glycan fragments and microbial contents that serve as ligands for immune receptors, which tailor the immune response against the offending pathogen. The glycan fragments serve as MHC class II (MHC II) ligands and innate receptor agonists, whereas microbial proteins ... More
Thrombin-induced activation of cultured rodent microglia.
AuthorsMöller T, Hanisch UK, Ransom BR
JournalJ Neurochem
PubMed ID10987834
'Microglia are the resident immune cells of the CNS. Upon brain damage, these cells are rapidly activated and function as tissue macrophages. The first steps in this activation still remain unclear, but it is widely believed that substances released from damaged brain tissue trigger this process. In this article, we ... More
Nitric oxide expression in the spleen, but not in the liver, correlates with resistance to blood-stage malaria in mice.
AuthorsJacobs P, Radzioch D, Stevenson MM
JournalJ Immunol
PubMed ID7594544
'The production and function of nitric oxide during the early phase of blood-stage infection with Plasmodium chabaudi AS was analyzed using two inbred strains of mice that differ in the level of resistance to this parasite. Northern blot analysis of in vivo expression of inducible nitric oxide synthase (iNOS) revealed ... More
Hydrogen peroxide-supported oxidation of NG-hydroxy-L-arginine by nitric oxide synthase.
AuthorsPufahl RA, Wishnok JS, Marletta MA
JournalBiochemistry
PubMed ID7531495
'The ability of murine macrophage nitric oxide synthase (NOS) to utilize peroxides in place of O2 and NADPH was investigated using hydrogen peroxide (H2O2), tert-butylhydroperoxide, and cumene hydroperoxide with both L-arginine and NG-hydroxy-L-arginine (L-NHA) as substrates. Of the three peroxides examined, only H2O2 was able to support product formation using ... More
Nitric oxide donation and nitrite assays in the presence of thiols and albumin as determined by Griess' and Werringloer's methods.
AuthorsRobak J, Marcinkiewicz E, Michalska Z, Gryglewski RJ
JournalPol J Pharmacol
PubMed ID9437769
'Nitric oxide (NO) or nitrite (NO2-) were assayed using the Werringloer''s method or the Griess'' method, respectively, in the presence or absence of various thiols, amino acids, or albumin. This has been done because both methods are used to determine the generation of endogenous NO from L-arginine or exogenous NO ... More
Is there a role for nitric oxide in regulation of T cell secretion of IL-2?
AuthorsMarcinkiewicz J, Grabowska A, Chain BM
JournalJ Immunol
PubMed ID8648104
'Nitric oxide (NO) can have both effector (cytotoxic) and regulatory roles in immune function. In this study, we have re-examined the potential role of nitric oxide in mediating the macrophage-dependent suppression of IL-2 synthesis. In our model, TNP-specific CD4+ T cells are cocultured with Ag and either peritoneal or alveolar ... More
Human monocytes induce a carcinoma cell line to secrete high amounts of nitric oxide.
AuthorsKonur A, Krause SW, Rehli M, Kreutz M, Andreesen R
JournalJ Immunol
PubMed ID8757334
'Nitric oxide (NO) is a short-lived pleiotropic mediator with a multitude of biologic functions. The inducible form of NO synthase (iNOS) is responsible for the discontinuous production of high amounts of NO and is important for the cytotoxic capacity of macrophages in rodents, whereas NO production by human macrophages or ... More
Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.
AuthorsSanthanam L, Gucek M, Brown TR, Mansharamani M, Ryoo S, Lemmon CA, Romer L, Shoukas AA, Berkowitz DE, Cole RN,
JournalNitric Oxide
PubMed ID18706513
'Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that ... More
S-nitrosohemoglobin in the fetal circulation may represent a cycle for blood pressure regulation.
AuthorsFunai EF, Davidson A, Seligman SP, Finlay TH
JournalBiochem Biophys Res Commun
PubMed ID9367862
'It has recently been demonstrated, in rats, that hemoglobin transports nitric oxide (NO), as S-nitrosocysteine, from the lungs to the peripheral tissues. This cycle may be involved in the regulation of blood pressure and efficient delivery of oxygen in adult animals. We sought to determine whether this model was applicable ... More
Diaminonaphthalenes and related aminocompounds: mutagenicity, CYP1A induction and interaction with the Ah receptor.
AuthorsCheung YL, Lewis DF, Ridd TI, Gray TJ, Ioannides C
JournalToxicology
PubMed ID9129166
'Using 1- and 2-aminonaphthalene as model substrates, we investigated the effect of insertion of a second amino group on mutagenicity, binding to the cytosolic Ah receptor and CYP1A inducibility, and the effects were compared to those elicited by 3,3''-diaminobenzidine and 1-naphthylethylenediamine. 1,5- and 1,8-diaminonaphthalene were effective inducers of CYP1A activity, ... More
M-1/M-2 macrophages and the Th1/Th2 paradigm.
AuthorsMills CD, Kincaid K, Alt JM, Heilman MJ, Hill AM
JournalJ Immunol
PubMed ID10843666
'Evidence is provided that macrophages can make M-1 or M-2 responses. The concept of M-1/M-2 fomented from observations that macrophages from prototypical Th1 strains (C57BL/6, B10D2) are more easily activated to produce NO with either IFN-gamma or LPS than macrophages from Th2 strains (BALB/c, DBA/2). In marked contrast, LPS stimulates ... More
Nitric oxide regulates interleukin 1 bioactivity released from murine macrophages.
AuthorsHill JR, Corbett JA, Kwon G, Marshall CA, McDaniel ML
JournalJ Biol Chem
PubMed ID8798439
'The bioactivity of interleukin-1 (IL-1), a major proinflammatory cytokine, can be modulated by a variety of factors including inhibitors of IL-1 production and release and receptor blockade by IL-1 receptor antagonist and by binding to nonsignaling soluble receptors. This study demonstrates that the free radical nitric oxide (NO) is also ... More
Fibroblasts as host cells in latent leishmaniosis.
AuthorsBogdan C, Donhauser N, Döring R, Röllinghoff M, Diefenbach A, Rittig MG
JournalJ Exp Med
PubMed ID10859337
'Intracellular parasites are known to persist lifelong in mammalian hosts after the clinical cure of the disease, but the mechanisms of persistence are poorly understood. Here, we show by confocal laser microscopy that in the draining lymph nodes of mice that had healed a cutaneous infection with Leishmania major, 40% ... More
Myoglobin-catalyzed tyrosine nitration: no need for peroxynitrite.
AuthorsKilinc K, Kilinc A, Wolf RE, Grisham MB
JournalBiochem Biophys Res Commun
PubMed ID11444837
'The nitration of tyrosine residues in protein to yield 3-nitrotyrosine derivatives has been suggested to represent a specific footprint for peroxynitrite formation in vivo. However, recent studies suggest that certain hemoproteins such as peroxidases catalyze the H(2)O(2)-dependent nitration of tyrosine to yield 3-nitrotyrosine in a peroxynitrite-independent reaction. Because 3-nitrotyrosine has ... More
Differential regulation of mast cell function by IL-10 and stem cell factor.
AuthorsLin TJ, Befus AD
JournalJ Immunol
PubMed ID9378991
'In the development of rodent mast cells (MC), IL-10 significantly enhances the growth factor activity of stem cell factor (SCF). The differential effects of IL-10 and SCF on function of rat peritoneal MC (PMC) are investigated in this study. IL-10 inhibits both constitutive and Ag-induced nitric oxide production by PMC ... More
Comparison of spectrophotometric and biological assays for nitric oxide (NO) and endothelium-derived relaxing factor (EDRF): nonspecificity of the diazotization reaction for NO and failure to detect EDRF.
AuthorsTracey WR, Linden J, Peach MJ, Johns RA
JournalJ Pharmacol Exp Ther
PubMed ID2319475
'Endothelium-derived relaxing factor has been tentatively identified as nitric oxide (NO) partially on the basis of chemical assays. In the present study, saline solutions that were either bubbled continuously for 30 min with NO (NO/X) or prepared using 25 ml of NO/ml (NO/25) produced equivalent relaxations of segments of rabbit ... More
Expression of serotonin receptors in bone.
AuthorsWestbroek I, van der Plas A, de Rooij KE, Klein-Nulend J, Nijweide PJ
JournalJ Biol Chem
PubMed ID11387323
'The 5-hydroxytryptamine (5-HT) receptors 5-HT(2A), 5-HT(2B), and 5-HT(2C) belong to a subfamily of serotonin receptors. Amino acid and mRNA sequences of these receptors have been published for several species including man. The 5-HT(2) receptors have been reported to act on nervous, muscle, and endothelial tissues. Here we report the presence ... More
Tumoricidal activity of endothelial cells. Inhibition of endothelial nitric oxide production abrogates tumor cytotoxicity induced by hepatic sinusoidal endothelium in response to B16 melanoma adhesion in vitro.
AuthorsCarretero J, Obrador E, Esteve JM, Ortega A, Pellicer JA, Sempere FV, Estrela JM
JournalJ Biol Chem
PubMed ID11313348
'The mechanism of NO- and H(2)O(2)-induced tumor cytotoxicity was examined during B16 melanoma (B16M) adhesion to the hepatic sinusoidal endothelium (HSE) in vitro. We used endothelial nitric-oxide synthetase gene disruption and N(G)-nitro-l-arginine methyl ester-induced inhibition of nitric-oxide synthetase activity to study the effect of HSE-derived NO on B16M cell viability. ... More
Effect of overexpression of human Cu,Zn superoxide dismutase in transgenic mice on macrophage functions.
AuthorsMirochnitchenko O, Inouye M
JournalJ Immunol
PubMed ID8568263
'Properties of macrophages from transgenic mice with the human Cu,Zn superoxide dismutase (SOD) gene under the control of the mouse hydroxyl-methyl coenzyme A reductase (HMGCR) promoter were studied. In these mice, a twofold overproduction of Cu,Zn SOD in intraperitoneal macrophages resulted in the significant reduction of their microbicidal and fungicidal ... More
Elevated production of salivary nitric oxide in oral mucosal diseases.
AuthorsOhashi M, Iwase M, Nagumo M
JournalJ Oral Pathol Med
PubMed ID10478960
'Nitric oxide (NO) is known to play an important role in biological systems. In this study, we measured levels of NO in the saliva of 39 patients with oral mucosal diseases: 21 had oral lichen planus (OLP) and 18 had recurrent aphthous ulceration (RAU). NO was assayed using the Griess ... More
Macrophage-stimulating protein inhibits induction of nitric oxide production by endotoxin- or cytokine-stimulated mouse macrophages.
AuthorsWang MH, Cox GW, Yoshimura T, Sheffler LA, Skeel A, Leonard EJ
JournalJ Biol Chem
PubMed ID7514598
'Human serum macrophage-stimulating protein (MSP) is a disulfide-linked heterodimer that induces motile and phagocytic activity of mouse resident peritoneal macrophages. In this work, we found that MSP blocked the increase in macrophage nitric oxide synthase mRNA, as well as the associated increase in nitric oxide production, that occurred in response ... More
Reevaluation of the Griess reaction: how much of a problem is interference by nicotinamide nucleotides?
AuthorsJohn Moody A, Shaw FL
JournalAnal Biochem
PubMed ID16797475
Modified microassay for serum nitrite and nitrate.
AuthorsVodovotz Y
JournalBiotechniques
PubMed ID8679195
Nitric-oxide synthase assays.
AuthorsHevel JM, Marletta MA
JournalMethods Enzymol
PubMed ID7516999
Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids.
AuthorsGreen LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR
JournalAnal Biochem
PubMed ID7181105
Determination of nitric oxide using fluorescence spectroscopy.
AuthorsMiles AM, Wink DA, Cook JC, Grisham MB
JournalMethods Enzymol
PubMed ID8782577
Nitrite and nitrate measurement by Griess reagent in human plasma: evaluation of interferences and standardization.
AuthorsGiustarini D, Rossi R, Milzani A, Dalle-Donne I,
JournalMethods Enzymol
PubMed ID18423230
Nitrite and nitrate represent the final products of nitric oxide (NO) oxidation pathways, and their hematic concentrations are frequently assessed as an index of systemic NO production. However, their intake with food can influence their levels. Nitrite and nitrate could have a role by producing NO, because nitrite can release ... More
In vitro and in vivo anti-inflammatory activity of 17-O-acetylacuminolide through the inhibition of cytokines, NF-?B translocation and IKKß activity.
AuthorsAchoui M, Appleton D, Abdulla MA, Awang K, Mohd MA, Mustafa MR,
JournalPLoS One
PubMed ID21152019
17-O-acetylacuminolide (AA), a diterpenoid labdane, was isolated for the first time from the plant species Neouvaria foetida. The anti-inflammatory effects of this compound were studied both in vitro and in vivo. Plant extracts were initially tested against LPS-stimulated release of tumor necrosis factor alpha (TNF-a) from murine macrophages (RAW264.7 cells). ... More
Cucurbitacin L 2-O-ß-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation.
AuthorsAbdelwahab SI, Hassan LE, Abdul Majid AM, Yagi SM, Mohan S, Elhassan Taha MM, Ahmad S, Chuen CS, Narrima P, Rais MM, Syam S, Moharam BA, Hadi AH,
JournalEvid Based Complement Alternat Med
PubMed ID22685485
Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. ... More
Outer membrane protein A (OmpA) binds to and activates human macrophages.
AuthorsSoulas C, Baussant T, Aubry JP, Delneste Y, Barillat N, Caron G, Renno T, Bonnefoy JY, Jeannin P
JournalJ Immunol
PubMed ID10946255
Outer membrane protein (Omp)A is highly represented and conserved in the Enterobacteriaceae family. Using a recombinant OmpA from Klebsiella pneumoniae (P40), we have analyzed the interaction between OmpA and macrophages. We report that Alexa488-labeled P40 binds (at 4 degrees C) to murine and human macrophages in a dose-dependent manner and ... More
Practical methods for detection of nitric oxide.
AuthorsNagano T
JournalLuminescence
PubMed ID10602296
Nitric oxide (NO), which is generated in vivo through conversion of L-arginine to L-citrulline by NO synthase (NOS), mediates many physiological and pathophysiological processes. At least two distinct isoforms of NOS have been identified, constitutive NOS (cNOS) and inducible NOS (iNOS). The cNOS, which is constitutively expressed in endothelial cells ... More
Bacterial lipopeptides induce nitric oxide synthase and promote apoptosis through nitric oxide-independent pathways in rat macrophages.
AuthorsTerenzi F, Díaz-Guerra MJ, Casado M, Hortelano S, Leoni S, Boscá L
JournalJ Biol Chem
PubMed ID7534305
Stimulation of resident peritoneal macrophages with S-[2,3-bis(pamitoyloxy)-(2R,2S)-propyl]-N-palmytoyl-(R)-C ysSerLys4 or S(-)[2,3-bis(pamitoyloxy)-(2R,2S)-propyl]-N-palmytoyl-(R)-++ +CysAlaLys4, two synthetic bacterial lipopeptides, promoted the expression of the inducible form of nitric oxide synthase, exhibiting a temporal pattern of nitric oxide release that was delayed with respect to the induction elicited by bacterial lipopolysaccharide. Treatment of macrophages with ... More
Biologic activities of the beta-chemokine TCA3 on neutrophils and macrophages.
AuthorsDevi S, Laning J, Luo Y, Dorf ME
JournalJ Immunol
PubMed ID7730638
Previous in vivo and in vitro studies demonstrated that the murine beta-chemokine TCA3 is a chemoattractant for monocytes/macrophages and neutrophils. The ability of TCA3 to activate these cell populations is now evaluated. Treatment with 10 to 20 nM rTCA3 induced a respiratory burst with the production of superoxide and hydrogen ... More
Induction of nitric oxide synthase mRNA expression. Suppression by exogenous nitric oxide.
AuthorsColasanti M, Persichini T, Menegazzi M, Mariotto S, Giordano E, Caldarera CM, Sogos V, Lauro GM, Suzuki H
JournalJ Biol Chem
PubMed ID7592903
The reactive nitrogen species, nitric oxide (NO), plays an important role in the pathogenesis of neurodegenerative diseases. The suppression of NO production may be fundamental for survival of neurons. Here, we report that pretreatment of human ramified microglial cells with nearly physiological levels of exogenous NO prevents lipopolysaccharide (LPS)/tumor necrosis ... More
Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrite.
AuthorsVerdon CP, Burton BA, Prior RL
JournalAnal Biochem
PubMed ID7733451
An assay for the simultaneous measurement of nitrite and nitrate, products of nitric oxide metabolism, is described. Others have reported pretreating sample by using nitrate reductase (NR) and NADPH to reduce endogenous NO3- before assaying the resultant NO2- using the Griess reaction. However, we found that the NADP+ formed during ... More
Cu,Zn-superoxide dismutase-dependent apoptosis induced by nitric oxide in neuronal cells.
AuthorsCiriolo MR, De Martino A, Lafavia E, Rossi L, Carrì MT, Rotilio G
JournalJ Biol Chem
PubMed ID10671549
Nitric oxide (NO) challenge to human neuroblastoma cells (SH-SY5Y) ultimately results in apoptosis. Tumor suppressor protein p53 and cell cycle inhibitor p21 accumulate as an early sign of S-nitrosoglutathione-mediated toxicity. Cytochrome c release from mitochondria and caspase 3 activation also occurred. Cells transfected with either wild type (WT) or mutant ... More
A spectrophotometric method for the direct detection and quantitation of nitric oxide, nitrite, and nitrate in cell culture media.
AuthorsRidnour LA, Sim JE, Hayward MA, Wink DA, Martin SM, Buettner GR, Spitz DR
JournalAnal Biochem
PubMed ID10870839
A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-colored product absorbing at 496 nm. Nitric oxide levels were determined in ... More
Inducible nitric oxide synthase requires both the canonical calmodulin-binding domain and additional sequences in order to bind calmodulin and produce nitric oxide in the absence of free Ca2+.
AuthorsRuan J, Xie Q, Hutchinson N, Cho H, Wolfe GC, Nathan C
JournalJ Biol Chem
PubMed ID8798440
All three mammalian isoforms of nitric oxide synthase (NOS) must bind calmodulin (CaM) for enzymatic activity. Only NOS2 (the inducible isoform, iNOS) does so at the low levels of free Ca2+ in resting cells and when almost all Ca2+ is chelated in cell-free preparations. To test directly whether the predicted ... More
IL-4 plays a crucial role in regulating oxidative damage in the liver during schistosomiasis.
AuthorsLa Flamme AC, Patton EA, Bauman B, Pearce EJ
JournalJ Immunol
PubMed ID11160238
Liver enlargement and hepatocyte proliferation, normal responses in wild-type (WT) mice infected with the parasitic helminth Schistosoma mansoni, were found to be severely impaired in infected IL-4(-/-) mice. Compared with WT mice, increased levels of O(2)(-), NO, and the more highly reactive ONOO(-) were detected in the liver and produced ... More
An apoptotic model for nitrosative stress.
AuthorsEu JP, Liu L, Zeng M, Stamler JS
JournalBiochemistry
PubMed ID10653649
Nitric oxide overproduction has been implicated in the pathogenesis of many disorders, including artherosclerosis, neurodegenerative diseases, inflammatory and autoimmune diseases, and cancer. The common view holds that nitric oxide-induced cellular injury is caused by oxidative stress. This theory predicts that interactions between reactive nitrogen species and reactive oxygen species produce ... More
Nitric oxide inhibits the tumor necrosis factor alpha -regulated endocytosis of human dendritic cells in a cyclic GMP-dependent way.
AuthorsPaolucci C, Rovere P, De Nadai C, Manfredi AA, Clementi E
JournalJ Biol Chem
PubMed ID10777484
Tumor necrosis factor-alpha (TNFalpha)-induced maturation of dendritic cells (DC), with down-regulation of their endocytic ability, has been reported to be mediated by the accumulation of the lipid messenger ceramide. We have now studied the effects and mechanisms of action of NO on endocytosis, investigated with fluorescein isothiocyanate-labeled dextran using human ... More
Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG.
AuthorsYang J, Kawamura I, Zhu H, Mitsuyama M
JournalJ Immunol
PubMed ID7499860
Viable and killed BCG were compared for the ability to induce nitric oxide (NO) production in normal spleen cells and peritoneal exudate macrophages. Stimulation of spleen cells with viable BCG resulted in a strong expression of inducible NO synthase followed by an enhanced production of nitrite. However, those responses were ... More
Lipopolysaccharide internalization activates endotoxin-dependent signal transduction in cardiomyocytes.
AuthorsCowan DB, Noria S, Stamm C, Garcia LM, Poutias DN, del Nido PJ, McGowan FX
JournalCirc Res
PubMed ID11249872
We tested the hypothesis that bacterial lipopolysaccharide (LPS) must be internalized to facilitate endotoxin-dependent signal activation in cardiac myocytes. Fluorescently labeled LPS was used to treat primary cardiomyocyte cultures, perfused heart preparations, and the RAW264.7 macrophage cell line. Using confocal microscopy and spectrofluorometry, we found that LPS was rapidly internalized ... More
Transforming growth factor-beta 1, but not dexamethasone, down-regulates nitric-oxide synthase mRNA after its induction by interleukin-1 beta in rat smooth muscle cells.
AuthorsPerrella MA, Yoshizumi M, Fen Z, Tsai JC, Hsieh CM, Kourembanas S, Lee ME
JournalJ Biol Chem
PubMed ID7514175
Nitric oxide (NO), which accounts for the biologic properties of endothelium-derived relaxing factor, is synthesized from L-arginine by nitric-oxide synthase (NOS). Two classes of NOS have been identified: a constitutive, calcium-dependent isozyme (cNOS) and an inducible, calcium-independent isozyme (iNOS). NO is generated after the induction of iNOS by cytokines such ... More
Effects of methotrexate on the oxidative metabolism of cultured rabbit articular chondrocytes.
AuthorsHayem G, Domarle O, Thuong-Guyot M, Pocidalo JJ, Meyer O
JournalJ Rheumatol
PubMed ID10813275
OBJECTIVE: The mechanism of action of methotrexate (MTX) in inflammatory joint disease is still unclear. We examined the possible interactions of MTX with the oxidative metabolism of rabbit articular chondrocytes. METHODS: Cell cultures of articular chondrocytes enzymatically isolated from juvenile New Zealand white rabbits were incubated 24 h with either ... More
IFN-alpha beta reconstitutes the deficiency in lipid A-activated AKR macrophages for nitric oxide synthase.
AuthorsJiang H, Rummage JA, Zhou A, Chen Z, Herriot MJ, Stewart CA, Kolosov M, Leu RW
JournalJ Immunol
PubMed ID8683131
AKR mouse peritoneal macrophages (PM) were previously found to have a defect in their response to lipid A for nitric oxide (NO)-mediated tumor cytotoxicity, which was related to a lower level of C1q synthesis and reconstituted by exogenous IFN-gamma or C1q. We used AKR-PM as a model to further define ... More
The nitric oxide congener nitrite inhibits myeloperoxidase/H2O2/ Cl- -mediated modification of low density lipoprotein.
AuthorsCarr AC, Frei B
JournalJ Biol Chem
PubMed ID11054430
Nitric oxide, a pivotal molecule in vascular homeostasis, is converted under aerobic conditions to nitrite. Recent studies have shown that myeloperoxidase (MPO), an abundant heme protein released by activated leukocytes, can oxidize nitrite (NO(2-)) to a radical species, most likely nitrogen dioxide. Furthermore, hypochlorous acid (HOCl), the major strong oxidant ... More
Ergothioneine rescues PC12 cells from beta-amyloid-induced apoptotic death.
AuthorsJang JH, Aruoma OI, Jen LS, Chung HY, Surh YJ
JournalFree Radic Biol Med
PubMed ID15036348
Beta-amyloid (Abeta) peptide is the major component of senile plaques and considered to have a causal role in the development and progression of Alzheimer's disease. There has been compelling evidence that Abeta-induced cytotoxicity is mediated through oxidative and/or nitrosative stress. Recently, considerable attention has been focused on dietary manipulation of ... More
Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and S-nitrosothiols.
AuthorsMarzinzig M, Nussler AK, Stadler J, Marzinzig E, Barthlen W, Nussler NC, Beger HG, Morris SM, Brückner UB
JournalNitric Oxide
PubMed ID9701056
The aim of this study was to compare and improve standard methods to determine nitrite (NO2-), nitrate (NO3-) and S-nitrosothiol (RSNO) levels in cell culture supernatants, sera, and urine. We modified the conventional Griess reaction by replacing sulfanilamide with dapsone (4,4'-diamino-diphenylsulfone) and compared the NO2- levels in our study samples ... More
Inhibition of the catalytic activity of alcohol dehydrogenase by nitric oxide is associated with S nitrosylation and the release of zinc.
AuthorsGergel D, Cederbaum AI
JournalBiochemistry
PubMed ID8973191
Nitric oxide (NO) reacts with the sulfhydryl groups of proteins to form nitroso thiols. Alcohol dehydrogenase (ADH) plays an important role in the metabolism of ethanol. Chronic alcohol administration stimulates NO formation in the liver, and production of NO is increased in alcohol liver injury. The effect of exogenous and ... More
Role of endogenous interferon-beta in lipopolysaccharide-triggered activation of the inducible nitric-oxide synthase gene in a mouse macrophage cell line, J774.
AuthorsFujihara M, Ito N, Pace JL, Watanabe Y, Russell SW, Suzuki T
JournalJ Biol Chem
PubMed ID7513694
The role of endogenous tumor necrosis factor alpha (TNF-alpha) and interferon-beta (IFN-beta) in lipopolysaccharide (LPS)-induced activation of the inducible nitric-oxide synthase (i-NOS) gene was investigated. By Northern analysis or reverse-transcription polymerase chain reaction, the mouse macrophage cell line (J774) was found to respond to LPS treatment by increased expression ... More
Peroxynitrite-mediated DNA strand breakage activates poly-adenosine diphosphate ribosyl synthetase and causes cellular energy depletion in macrophages stimulated with bacterial lipopolysaccharide.
AuthorsZingarelli B, O'Connor M, Wong H, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID8598485
The inducible isoform of nitric oxide (NO) synthase produces large quantities of NO, a cytotoxic free radical. Recent studies show that treatment with exogenous NO produces DNA strand breaks, activating the nuclear repair enzyme poly(ADP)ribosyltransferase (PARS), which results in ADP ribosylation, NAD+ consumption, and exhaustion of intracellular energy stores. Here ... More
Convenient colorimetric and fluorometric assays for S-nitrosothiols.
AuthorsCook JA, Kim SY, Teague D, Krishna MC, Pacelli R, Mitchell JB, Vodovotz Y, Nims RW, Christodoulou D, Miles AM, Grisham MB, Wink DA
JournalAnal Biochem
PubMed ID8660604
S-nitrosothiols have been shown to affect a number of physiological functions. Several techniques have been used to detect these species in biological systems, primarily by methods utilizing chemiluminescence. Since the apparatus required for measurement of chemiluminescence are not readily available in most laboratories, methods employing more conventional techniques such as ... More
Cytokines and insulin induce cationic amino acid transporter (CAT) expression in cardiac myocytes. Regulation of L-arginine transport and no production by CAT-1, CAT-2A, and CAT-2B.
AuthorsSimmons WW, Closs EI, Cunningham JM, Smith TW, Kelly RA
JournalJ Biol Chem
PubMed ID8662674
Cytokine-dependent production of nitric oxide (NO) by rat cardiac myocytes is a consequence of increased expression of the inducible isoform of nitric oxide synthase (iNOS or NOS2) and, in the presence of insulin, depresses the contractile function of these cells in vivo and in vitro. Experiments reported here show that ... More
Induction of nitric oxide in human monocytes and monocyte cell lines by Mycobacterium tuberculosis.
AuthorsJagannath C, Actor JK, Hunter RL
JournalNitric Oxide
PubMed ID9731635
The induction of nitric oxide in human monocytes during mycobacterial infection has been a controversial issue. This study describes a comparative evaluation of the colorimetric and fluorometric methods for the detection of NO in response to Mycobacterium tuberculosis (MTB) infection in human peripheral blood-derived monocytes (PBM) and in U937, a ... More
NOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury.
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JournalJ Neuroinflammation
PubMed ID28340575
Wetting ridge assisted programmed magnetic actuation of droplets on ferrofluid-infused surface.
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JournalNat Commun
PubMed ID34880250
Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes.
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JournalCell
PubMed ID33278357
Nitric oxide orchestrates metabolic rewiring in M1 macrophages by targeting aconitase 2 and pyruvate dehydrogenase.
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JournalNat Commun
PubMed ID32019928
NOD1 activators link innate immunity to insulin resistance.
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JournalDiabetes
PubMed ID21715553
Immunosuppressive Myeloid Cells Induce Nitric Oxide-Dependent DNA Damage and p53 Pathway Activation in CD8+ T Cells.
HDAC6 Mediates Macrophage iNOS Expression and Excessive Nitric Oxide Production in the Blood During Endotoxemia.
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JournalFront Immunol
PubMed ID32973784
Evidence against Stable Protein S-Nitrosylation as a Widespread Mechanism of Post-translational Regulation.
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JournalMol Cell
PubMed ID29358077
Coronary Serum Exosomes Derived from Patients with Myocardial Ischemia Regulate Angiogenesis through the miR-939-mediated Nitric Oxide Signaling Pathway.