HCS 线粒体健康检测试剂盒

引用和文献 (14)

Invitrogen™

HCS 线粒体健康检测试剂盒

HCS 线粒体健康检测试剂盒能够同时定量测定两个重要的细胞健康参数：线粒体毒性和细胞毒性。MitoHealth 染料可检测线粒体膜电位的变化，然后试剂在活性线粒体中累积。试剂盒包括可用于检测细胞毒性的 Image-iT® DEAD™ Green，这种染料不可透过健康细胞，但会透过细胞膜受损后的细胞。两种指示剂都适用于固定和透化，因此能够与其他感兴趣的生物标记物一起进行多通路检测。还包含蓝色荧光细胞核分隔工具了解更多信息

货号	数量
H10295	1 kit

货号 H10295

价格（CNY)

6,300.00

添加至购物车

1 kit

价格（CNY)

6,300.00

添加至购物车

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型Image-iT™ DEAD Green 细胞活力染色剂、MitoHealth 染色剂、Hoechst 33342

产品规格96 孔板

数量1 kit

运输条件室温

颜色橙色、蓝色、绿色

适用于（设备）荧光显微镜, 高内涵分析仪

产品线Molecular Probes

产品类型线粒体健康测定试剂盒

Unit SizeEach

内容与储存

试剂盒包含足够测定96孔板规格的2块微孔板的试剂。

在 ≤-20°C 下干燥、避光储存。

常见问题解答 (FAQ)

What is the fluorescence excitation/emission maxima of the stains in the HCS mitochondrial health kit?

The fluorescence excitation/emission maxima are as follows: Hoechst 33342 - 350/461 nm bound to DNA; Image-iT DEAD Green viability stain - 488/515 nm; MitoHealth stain - 550/580 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (14)

引用和文献

Abstract

Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro.

Authors:Alileche A, Goswami J, Bourland W, Davis M, Hampikian G,

Journal:Peptides

PubMed ID:23000474

'We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to ... More

High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.

Authors:Qu D, Gu Y, Feng L, Han J

Journal:Food Chem

PubMed ID:28530558

'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and ... More

Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.

Authors:Blajszczak CC, Nonn L

Journal:J Steroid Biochem Mol Biol

PubMed ID:31574299

'Vitamin D deficiency has been associated with increased risk for aggressive prostate cancer (PCa). Prostate epithelium has a unique metabolism compared to other tissues. Normal prostate exhibits low levels of mitochondrial respiration and there is a metabolic switch to increased oxidative phosphorylation in PCa. 25-hydroxyvitamin D (25(OH)D) is the major ... More

Handling and Assessment of Human Primary Prostate Organoid Culture.

Authors:McCray T, Richards Z, Marsili J, Prins GS, Nonn L,

Journal:J Vis Exp

PubMed ID:30735176

'This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing ... More

A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells.

Authors:Joossens E, Macko P, Palosaari T, Gerloff K, Ojea-Jiménez I, Gilliland D, Novak J, Fortaner Torrent S, Gineste JM, Römer I, Briffa SM, Valsami-Jones E, Lynch I, Whelan M,

Journal:Sci Data

PubMed ID:31048742

'The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information ... More

View all HCS 线粒体健康检测试剂盒 citations