Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro.
AuthorsAlileche A, Goswami J, Bourland W, Davis M, Hampikian G,
JournalPeptides
PubMed ID23000474
'We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to ... More
High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.
AuthorsQu D, Gu Y, Feng L, Han J
JournalFood Chem
PubMed ID28530558
'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and ... More
Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.
AuthorsBlajszczak CC, Nonn L
JournalJ Steroid Biochem Mol Biol
PubMed ID31574299
'Vitamin D deficiency has been associated with increased risk for aggressive prostate cancer (PCa). Prostate epithelium has a unique metabolism compared to other tissues. Normal prostate exhibits low levels of mitochondrial respiration and there is a metabolic switch to increased oxidative phosphorylation in PCa. 25-hydroxyvitamin D (25(OH)D) is the major ... More
Handling and Assessment of Human Primary Prostate Organoid Culture.
'This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing ... More
A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells.
AuthorsJoossens E, Macko P, Palosaari T, Gerloff K, Ojea-Jiménez I, Gilliland D, Novak J, Fortaner Torrent S, Gineste JM, Römer I, Briffa SM, Valsami-Jones E, Lynch I, Whelan M,
JournalSci Data
PubMed ID31048742
'The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information ... More
Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A).
Authors
JournalPLoS Pathog
PubMed ID23696738
Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model.
Authors
JournalCurr Protoc Neurosci
PubMed ID33147381
Fibroblast Biomarkers of Sporadic Parkinson's Disease and LRRK2 Kinase Inhibition.
It has been uncertain whether specific disease-relevant biomarker phenotypes can be found using sporadic Parkinson's disease (PD) patient-derived samples, as it has been proposed that there may be a plethora of underlying causes and pathological mechanisms. Fibroblasts derived from familial PD patients harboring leucine-rich repeat kinase 2 (LRRK2), PTEN-induced putative ... More
Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis.
AuthorsMandavilli BS, Aggeler RJ, Chambers KM
JournalMethods Mol Biol
PubMed ID29082485
High content screening (HCS)-based multiparametric measurements are very useful in early toxicity testing and safety assessment during drug development, and useful in evaluating the impact from new food supplements and environmental toxicants. Mitochondrial membrane potential, plasma membrane permeability, oxidative stress, phosphoplipidosis, and steatosis are a few of the important markers ... More
Deletion of the Mitochondrial Complex-IV Cofactor Heme A:Farnesyltransferase Causes Focal Segmental Glomerulosclerosis and Interferon Response.
AuthorsBaek JH, Gomez IG, Wada Y, Roach A, Mahad D, Duffield JS,
JournalAm J Pathol
PubMed ID30268775
Mutations in mitochondrial DNA as well as nuclear-encoded mitochondrial proteins have been reported to cause tubulointerstitial kidney diseases and focal segmental glomerulosclerosis (FSGS). Recently, genes and pathways affecting mitochondrial turnover and permeability have been implicated in adult-onset FSGS. Furthermore, dysfunctioning mitochondria may be capable of engaging intracellular innate immune-sensing pathways. ... More
Ag-NPs induce apoptosis, mitochondrial damages and MT3/OSGIN2 expression changes in an in vitro model of human dental-pulp-stem-cells-derived neurons.
AuthorsBonaventura G, La Cognata V, Iemmolo R, Zimbone M, Contino A, Maccarrone G, Failla B, Barcellona ML, Conforti FL, D'Agata V, Cavallaro S,
JournalNeurotoxicology
PubMed ID29698629
Silver nanoparticles (Ag-NPs) are one of the most popular nanotechnologies because of their unique antibacterial and antifungal properties. Given their increasing use in a wide range of commercial, biomedical and food products, exposure to Ag-NPs is now a reality in people's lives. However, there is a serious lack of information ... More
Synergistic Effects of The Enhancements to Mitochondrial ROS, p53 Activation and Apoptosis Generated by Aspartame and Potassium Sorbate in HepG2 Cells.
AuthorsQu D, Jiang M, Huang D, Zhang H, Feng L, Chen Y, Zhu X, Wang S, Han J
JournalMolecules
PubMed ID30696035
The safety of food additives has been widely concerned. Using single additives in the provisions of scope is safe, but the combination of additives, may induce additive, synergy, antagonism and other joint effects. This study investigated the cytotoxicity of aspartame (AT) together with potassium sorbate (PS). Thiazolyl Blue Tetrazolium Bromide ... More
Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions.
AuthorsWang R, Dong Y, Lu Y, Zhang W, Brann DW, Zhang Q
JournalMol Neurobiol
PubMed ID29951942
Hypothermia is currently the only approved therapy for global cerebral ischemia (GCI) after cardiac arrest; however, it unfortunately has multiple adverse effects. As a noninvasive procedure, photobiomodulation (PBM) therapy has emerged as a potential novel treatment for brain injury. PBM involves the use of low-level laser light therapy to influence ... More
After In Vitro Digestion, Jackfruit Flake Affords Protection against Acrylamide-Induced Oxidative Damage.
AuthorsQu D, Liu C, Jiang M, Feng L, Chen Y, Han J
JournalMolecules
PubMed ID31547332
Some studies have demonstrated that acrylamide (AA) was correlated with oxidative stress, resulting in physical damage. The jackfruit flake was an immature pulp that contained a high level of antioxidant activity. This study aimed to assess the defensive efficacy of jackfruit flake in AA-induced oxidative stress before and after simulated ... More