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查看更多产品信息 Hypoxia Green Reagent for Flow Cytometry - FAQs (H20035)
4 个常见问题解答
Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.