HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit, for high-content screening, for cellular imaging - FAQs

I'm seeing aggregates in my thawed LipidTox stain solutions. Is this okay?

After thawing LipidTOX Red phospholipidosis detection reagent, some minute aggregates might be observed in the solution. They usually will disappear if the vial is incubated in a 37 degrees C water bath for 5 minutes. These aggregates do not affect the performance of the assay.
Any aggregates that remain after the stain is diluted in media are removed by 0.2 µm filtration before the labeling solution is added to the cells, as recommended in the protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.