Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
Authors:Grandl M, Schmitz G,
Journal:Cytometry A
PubMed ID:20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
In vitro detection of drug-induced phospholipidosis using gene expression and fluorescent phospholipid based methodologies.
Authors:Nioi P, Perry BK, Wang EJ, Gu YZ, Snyder RD
Journal:Toxicol Sci
PubMed ID:17567588
'Phospholipidosis (PLD) is characterized by the excessive intracellular accumulation of phospholipids. It is well established that a large number of cationic amphiphilic drugs have the potential to induce PLD. In the present study, we describe two facile in vitro methods to determine the PLD-inducing potential of a molecule. The first ... More
Comparative analysis of lipotoxicity induced by endocrine, pharmacological, and innate immune stimuli in rat basophilic leukemia cells.
Authors:
Journal:J Immunotoxicol
PubMed ID:25539471
Time-restricted feeding delays the emergence of the age-associated, neoplastic-prone tissue landscape.
Authors:
Journal:Aging (Albany NY)
PubMed ID:31188781
Silencing of solute carrier family 13 member 5 disrupts energy homeostasis and inhibits proliferation of human hepatocarcinoma cells.