Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
In vitro detection of drug-induced phospholipidosis using gene expression and fluorescent phospholipid based methodologies.
AuthorsNioi P, Perry BK, Wang EJ, Gu YZ, Snyder RD
JournalToxicol Sci
PubMed ID17567588
'Phospholipidosis (PLD) is characterized by the excessive intracellular accumulation of phospholipids. It is well established that a large number of cationic amphiphilic drugs have the potential to induce PLD. In the present study, we describe two facile in vitro methods to determine the PLD-inducing potential of a molecule. The first ... More
Comparative analysis of lipotoxicity induced by endocrine, pharmacological, and innate immune stimuli in rat basophilic leukemia cells.
Authors
JournalJ Immunotoxicol
PubMed ID25539471
Time-restricted feeding delays the emergence of the age-associated, neoplastic-prone tissue landscape.
Authors
JournalAging (Albany NY)
PubMed ID31188781
Silencing of solute carrier family 13 member 5 disrupts energy homeostasis and inhibits proliferation of human hepatocarcinoma cells.
Authors
JournalJ Biol Chem
PubMed ID28655760
ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.
Authors
JournalJ Cell Sci
PubMed ID32843575
High Content Image Analysis as a Tool to Morphologically Distinguish Macrophage Activation and Determine Its Importance for Foamy Alveolar Macrophage Responses.