HCS LipidTOX™ 红色中性脂质染料，用于细胞成像

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引用和文献 (16)

Invitrogen™

HCS LipidTOX™ 红色中性脂质染料，用于细胞成像

中性脂质在细胞内的累积（脂肪变性）往往是由影响脂肪酸和/或中性脂质代谢的药物引发的。HCS LipidTOX™ 红色中性脂质染料开发用于表征化合物对哺乳动物细胞系中脂质代谢的潜在毒性作用。LipidTOX™ 中性脂质染料对中性脂质微滴具有极高的亲和力，可通过荧光显微镜或 HCS 读数仪进行检测了解更多信息

货号	数量
H34476	1 each

货号 H34476

价格（CNY)

4,485.00

Ends: 31-Dec-2025

6,079.00

共减 1,594.00 (26%)

添加至购物车

1 each

价格（CNY)

4,485.00

Ends: 31-Dec-2025

6,079.00

共减 1,594.00 (26%)

添加至购物车

中性脂质在细胞内的累积（脂肪变性）往往是由影响脂肪酸和/或中性脂质代谢的药物引发的。HCS LipidTOX™ 红色中性脂质染料开发用于表征化合物对哺乳动物细胞系中脂质代谢的潜在毒性作用。LipidTOX™ 中性脂质染料对中性脂质微滴具有极高的亲和力，可通过荧光显微镜或 HCS 读数仪进行检测。该探针与 HCS LipidTOX™ 磷脂沉积检测试剂兼容（H34350、H34351）。HCS LipidTOX™ 中性脂质染料也可用于监测脂肪细胞的形成和分化，该过程被称为脂肪生成。

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）高内涵分析仪

产品线LipidTOX

数量1 each

运输条件室温

标签类型其他标签或染料

产品类型脂质染料

亚细胞定位Cell Membranes and Lipids , Lipids

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我想标记细胞膜，但可选的染料有多种，该如何选择？

如果进行活细胞成像，CellVue和CellMask 质膜染色剂染色最均匀，被细胞内吞的速度最慢。如果想固定和通透细胞（如进行抗体标记），这种产品并不是最佳选择。麦胚凝集素（WGA）偶联物也能标记活细胞，或者甲醛固定的细胞。它们可以在随后的去垢剂（如TritonX-100）通透处理后保存下来。但如果细胞已经进行通透处理，WGA也会标记内部结构。因此，如果细胞已通透，则只能使用靶向质膜蛋白的抗体。亲脂性花青染料（如DiI）会标记活细胞的所有膜结构，而不仅仅是细胞质膜。此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html）的内容可帮助您进行选择。

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (16)

引用和文献

Abstract

Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.

Authors:Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI,

Journal:Circ Res

PubMed ID:19265037

'Lipid accumulation in arteries induces vascular inflammation and atherosclerosis, the major cause of heart attack and stroke in humans. Extreme hyperlipidemia induced in mice and rabbits enables modeling many aspects of human atherosclerosis, but microscopic examination of plaques is possible only postmortem. Here we report that feeding adult zebrafish (Danio ... More

Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.

Authors:Brennan L, Blanco Fernández A, Mostaert AS, Owende P,

Journal:J Microbiol Methods

PubMed ID:22521923

'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More

Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.

Authors:Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,

Journal:Eukaryot Cell

PubMed ID:20562225

'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More

Knockdown of ACAT-1 reduces amyloidogenic processing of APP.

Authors:Huttunen HJ, Greco C, Kovacs DM

Journal:FEBS Lett

PubMed ID:17412327

'Previous studies have shown that acyl-coenzyme A:cholesterol acyl transferase (ACAT), an enzyme that controls cellular equilibrium between free cholesterol and cholesteryl esters, modulates proteolytic processing of APP in cell-based and animal models of Alzheimer''s disease. Here we report that ACAT-1 RNAi reduced cellular ACAT-1 protein by approximately 50% and cholesteryl ... More

Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.

Authors:Pereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,

Journal:Biotechnol Biofuels

PubMed ID:22192119

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More

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