Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.
AuthorsStoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI,
JournalCirc Res
PubMed ID19265037
'Lipid accumulation in arteries induces vascular inflammation and atherosclerosis, the major cause of heart attack and stroke in humans. Extreme hyperlipidemia induced in mice and rabbits enables modeling many aspects of human atherosclerosis, but microscopic examination of plaques is possible only postmortem. Here we report that feeding adult zebrafish (Danio ... More
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.
AuthorsBrennan L, Blanco Fernández A, Mostaert AS, Owende P,
JournalJ Microbiol Methods
PubMed ID22521923
'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More
Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.
AuthorsWork VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,
JournalEukaryot Cell
PubMed ID20562225
'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More
Knockdown of ACAT-1 reduces amyloidogenic processing of APP.
AuthorsHuttunen HJ, Greco C, Kovacs DM
JournalFEBS Lett
PubMed ID17412327
'Previous studies have shown that acyl-coenzyme A:cholesterol acyl transferase (ACAT), an enzyme that controls cellular equilibrium between free cholesterol and cholesteryl esters, modulates proteolytic processing of APP in cell-based and animal models of Alzheimer''s disease. Here we report that ACAT-1 RNAi reduced cellular ACAT-1 protein by approximately 50% and cholesteryl ... More
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
AuthorsPereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,
JournalBiotechnol Biofuels
PubMed ID22192119
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More
In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules.
AuthorsLee DH, Bae CY, Han JI, Park JK,
Journal
PubMed ID24007509
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source and carbon dioxide as an only carbon source, have been considered as a feedstock of choice for the production of biofuels such as biodiesel. To explore the economic feasibility of such application, however, many technical hurdles ... More
A PPARγ transcriptional cascade directs adipose progenitor cell-niche interaction and niche expansion.
Authors
JournalNat Commun
PubMed ID28649987
A Neuronal Relay Mediates a Nutrient Responsive Gut/Fat Body Axis Regulating Energy Homeostasis in Adult Drosophila.
Authors
JournalCell Metab
PubMed ID30344016
Induced Pluripotent Stem Cell Differentiation Enables Functional Validation of GWAS Variants in Metabolic Disease.
Authors
JournalCell Stem Cell
PubMed ID28388431
The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.
Authors
JournalMol Cell
PubMed ID28890335
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Authors
JournalMol Cell
PubMed ID31628041
Glutamine Links Obesity to Inflammation in Human White Adipose Tissue.
Authors
JournalCell Metab
PubMed ID31866443
A PRDM16-Driven Metabolic Signal from Adipocytes Regulates Precursor Cell Fate.
Authors
JournalCell Metab
PubMed ID31155495
Sympathetic neuron-associated macrophages contribute to obesity by importing and metabolizing norepinephrine.
Authors
JournalNat Med
PubMed ID29035364
DEPTOR cell-autonomously promotes adipogenesis, and its expression is associated with obesity.
Authors
JournalCell Metab
PubMed ID22883231
A FoxA2+ long-term stem cell population is necessary for growth plate cartilage regeneration after injury.