'The prevention of Rhesus D alloimmunization through Rh immune globulin (RhIg) administration is the major indication for the accurate detection and quantification of fetomaternal hemorrhage (FMH). In the setting of D incompatibility, D-positive fetal cells can sensitize the D-negative mother, resulting in maternal anti-D alloantibody production. These anti-D alloantibodies may ... More
Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease.
AuthorsZou J, Mali P, Huang X, Dowey SN, Cheng L,
JournalBlood
PubMed ID21881051
'Human induced pluripotent stem cells (iPSCs) bearing monogenic mutations have great potential for modeling disease phenotypes, screening candidate drugs, and cell replacement therapy provided the underlying disease-causing mutation can be corrected. Here, we report a homologous recombination-based approach to precisely correct the sickle cell disease (SCD) mutation in patient-derived iPSCs ... More
Estimation of
AuthorsRoys JL, Warzynski MJ,
JournalCytometry B Clin Cytom
PubMed ID16059876
Improving laboratory practice: looking at fetal hemoglobin histograms.
AuthorsWarzynski MJ, Roys JL,
JournalCytometry B Clin Cytom
PubMed ID14994377
Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen-mismatched RBCs.
Anemia, a common condition among critically ill premature infants, is affected by red blood cell (RBC) survival (RCS). We hypothesized that transfused allogeneic Kidd antigen-mismatched RBCs would demonstrate the same concurrent RCS tracking as RBCs multilabeled at separate, discrete low densities with biotin (BioRBCs). Allogeneic RBCs from adult donors were ... More
Evaluation of F cells in sickle cell disorders by flow cytometry -- comparison with the Kleihauer-Betke's slide method.
AuthorsItalia KY, Colah R, Mohanty D,
JournalInt J Lab Hematol
PubMed ID17988294
Adult F cell numbers are raised in inherited haemoglobin disorders, such as beta-thalassaemia and sickle cell anaemia, hereditary persistence of foetal haemoglobin, and some acquired conditions, such as juvenile myelomonocytic leukaemia, during acute erythropoietic stress and pregnancy. True foetal erythrocytes containing foetal amounts of HbF can also occur in the ... More