'As low molecular weight proteins, restriction from glomerular filtration is minimized, permitting significant amounts of Ig light chains to be endocytosed into the proximal tubule epithelium, particularly in plasma cell dyscrasias. Recent studies have shown that this effect of concentrating light chains within the proximal tubule alters cell function. This ... More
Estrogen prevents cardiomyocyte apoptosis through inhibition of reactive oxygen species and differential regulation of p38 kinase isoforms.
AuthorsKim JK, Pedram A, Razandi M, Levin ER
JournalJ Biol Chem
PubMed ID16407188
'From human and animal studies, estrogen is known to protect the myocardium from an ischemic insult. However, there is limited knowledge regarding mechanisms by which estrogen directly protects cardiomyocytes. In this report, we employed an in vitro model, in which cultured rat cardiomyocytes underwent prolonged hypoxia followed by reoxygenation (H/R), ... More
24-dehydrocholesterol reductase/seladin-1: a key protein differentially involved in adrenocorticotropin effects observed in human and rat adrenal cortex.
AuthorsBattista MC, Roberge C, Martinez A, Gallo-Payet N,
JournalEndocrinology
PubMed ID19520779
'DHCR24 (24-dehydrocholesterol reductase), or seladin-1, is one of the most expressed genes in the adrenal gland. Because the rat and human adult adrenal cortex differ in their respective functional properties, the aim of the present study was to verify whether seladin-1 may be differentially involved in basal and ACTH-stimulated steroidogenesis ... More
Tubacin kills Epstein-Barr virus (EBV)-Burkitt lymphoma cells by inducing reactive oxygen species and EBV lymphoblastoid cells by inducing apoptosis.
AuthorsKawada J, Zou P, Mazitschek R, Bradner JE, Cohen JI,
JournalJ Biol Chem
PubMed ID19386607
'Tubacin is a small molecule inhibitor of histone deacetylase 6 and blocks aggresome activity. We found that Epstein-Barr virus (EBV)-positive Burkitt lymphoma (BL) cells were generally killed by lower doses of tubacin than EBV-transformed lymphoblastoid cells (LCLs) or EBV-negative BL cells. Tubacin induced apoptosis of LCLs, which was inhibited by ... More
HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.
AuthorsBollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP,
JournalCancer Res
PubMed ID20736364
'Activated oncogenes are the dominant drivers of malignant progression in human cancer, yet little is known about how the transformation from proto-oncogene to activated oncogene drives the expression of transformed phenotypes. An isogenic model of HER-2-mediated transformation of human mammary epithelial cells was used along with HER-2-amplified human breast cancers ... More
Platelet activation attracts a subpopulation of effector monocytes to sites of Leishmania major infection.
AuthorsGoncalves R, Zhang X, Cohen H, Debrabant A, Mosser DM,
JournalJ Exp Med
PubMed ID21606505
'Leishmania species trigger a brisk inflammatory response and efficiently induce cell-mediated immunity. We examined the mechanisms whereby leukocytes were recruited into lesions after Leishmania major infection of mice. We found that a subpopulation of effector monocytes expressing the granulocyte marker GR1 (Ly6C) is rapidly recruited into lesions, and these monocytes ... More
G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis.
AuthorsJun HS, Lee YM, Song KD, Mansfield BC, Chou JY,
JournalBlood
PubMed ID21292774
'G6PC3 (or glucose-6-phosphatase-ß) deficiency underlies a congenital neutropenia syndrome in which neutrophils exhibit enhanced endoplasmic reticulum (ER) stress, increased apoptosis, impaired energy homeostasis, and impaired functionality. Here we show that murine G6pc3(-/-) neutrophils undergoing ER stress activate protein kinase-like ER kinase and phosphatidylinositol 3,4,5-trisphosphate/Akt signaling pathways, and that neutrophil apoptosis ... More
Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways.
AuthorsMüller L, Riediker M, Wick P, Mohr M, Gehr P, Rothen-Rutishauser B,
JournalJ R Soc Interface
PubMed ID19586954
'Combustion-derived and manufactured nanoparticles (NPs) are known to provoke oxidative stress and inflammatory responses in human lung cells; therefore, they play an important role during the development of adverse health effects. As the lungs are composed of more than 40 different cell types, it is of particular interest to perform ... More
Antimicrobial peptaibols from Trichoderma pseudokoningii induce programmed cell death in plant fungal pathogens.
AuthorsShi M, Chen L, Wang XW, Zhang T, Zhao PB, Song XY, Sun CY, Chen XL, Zhou BC, Zhang YZ,
JournalMicrobiology
PubMed ID22053006
'Antibiosis is one of the widespread strategies used by Trichoderma spp. against plant fungal pathogens, the mechanism of which, however, remains poorly understood. Peptaibols are a large family of antimicrobial peptides produced by Trichoderma spp. Our previous study showed that trichokonins, a type of peptaibol from Trichoderma pseudokoningii SMF2, exhibited ... More
Histones activate the NLRP3 inflammasome in Kupffer cells during sterile inflammatory liver injury.
AuthorsHuang H, Chen HW, Evankovich J, Yan W, Rosborough BR, Nace GW, Ding Q, Loughran P, Beer-Stolz D, Billiar TR, Esmon CT, Tsung A,
Journal
PubMed ID23904166
Cellular processes that drive sterile inflammatory injury after hepatic ischemia/reperfusion (I/R) injury are not completely understood. Activation of the inflammasome plays a key role in response to invading intracellular pathogens, but mounting evidence suggests that it also plays a role in inflammation driven by endogenous danger-associate molecular pattern molecules released ... More
Exendin-4 protects endothelial cells from lipoapoptosis by PKA, PI3K, eNOS, p38 MAPK, and JNK pathways.
AuthorsErdogdu O, Eriksson L, Xu H, Sjöholm A, Zhang Q, Nyström T,
JournalJ Mol Endocrinol
PubMed ID23343509
Experimental studies have indicated that endothelial cells play an important role in maintaining vascular homeostasis. We previously reported that human coronary artery endothelial cells (HCAECs) express the glucagon-like peptide 1 (GLP1) receptor and that the stable GLP1 mimetic exendin-4 is able to activate the receptor, leading to increased cell proliferation. ... More
Epicatechin limits renal injury by mitochondrial protection in cisplatin nephropathy.
AuthorsTanabe K, Tamura Y, Lanaspa MA, Miyazaki M, Suzuki N, Sato W, Maeshima Y, Schreiner GF, Villarreal FJ, Johnson RJ, Nakagawa T,
JournalAm J Physiol Renal Physiol
PubMed ID22933302
Cisplatin nephropathy can be regarded as a mitochondrial disease. Intervention to halt such deleterious injury is under investigation. Recently, the flavanol (-)-epicatechin emerges as a novel compound to protect the cardiovascular system, owing in part to mitochondrial protection. Here, we have hypothesized that epicatechin prevents the progression of cisplatin-induced kidney ... More
Nicorandil as a novel therapy for advanced diabetic nephropathy in the eNOS-deficient mouse.
Nicorandil is an orally available drug that can act as a nitric oxide donor, an antioxidant, and an ATP-dependent K channel activator. We hypothesized that it may have a beneficial role in treating diabetic nephropathy. We administered nicorandil to a model of advanced diabetic nephropathy (the streptozotocin-induced diabetes in mice ... More
The role of nuclear lamin B1 in cell proliferation and senescence.
AuthorsShimi T, Butin-Israeli V, Adam SA, Hamanaka RB, Goldman AE, Lucas CA, Shumaker DK, Kosak ST, Chandel NS, Goldman RD,
JournalGenes Dev
PubMed ID22155925
Nuclear lamin B1 (LB1) is a major structural component of the nucleus that appears to be involved in the regulation of many nuclear functions. The results of this study demonstrate that LB1 expression in WI-38 cells decreases during cellular senescence. Premature senescence induced by oncogenic Ras also decreases LB1 expression ... More
Reduced redox potential of the cytosol is important for African swine fever virus capsid assembly and maturation.
AuthorsCobbold C, Windsor M, Parsley J, Baldwin B, Wileman T,
JournalJ Gen Virol
PubMed ID17170439
Assembly of African swine fever virus (ASFV) involves the transfer of the major capsid protein, p73, from the cytosol onto the cytoplasmic face of endoplasmic reticulum-derived membranes. During this process, the folding of p73 is dependent upon transient association with a specific viral chaperone, CAP80. The cell cytoplasm maintains high ... More
Systemic transplantation of c-kit+ cells exerts a therapeutic effect in a model of amyotrophic lateral sclerosis.
AuthorsCorti S, Nizzardo M, Nardini M, Donadoni C, Salani S, Simone C, Falcone M, Riboldi G, Govoni A, Bresolin N, Comi GP,
JournalHum Mol Genet
PubMed ID20650960
Amyotrophic lateral sclerosis (ALS) is a progressive, fatal, neurodegenerative disease characterized by the loss of motor neurons. Motor neuron degeneration is probably both a cell autonomous and a non-autonomous event. Therefore, manipulating the diseased microenvironment via non-neural cell replacement could be a therapeutic strategy. We investigated a cell therapy approach ... More
Silver nanoparticles disrupt GDNF/Fyn kinase signaling in spermatogonial stem cells.
AuthorsBraydich-Stolle LK, Lucas B, Schrand A, Murdock RC, Lee T, Schlager JJ, Hussain SM, Hofmann MC,
JournalToxicol Sci
PubMed ID20488942
Silver nanoparticles (Ag-NPs) are being utilized in an increasing number of fields and are components of antibacterial coatings, antistatic materials, superconductors, and biosensors. A number of reports have now described the toxic effects of silver nanoparticles on somatic cells; however, no study has examined their effects on the germ line ... More
Deceleration of senescence in normal human fibroblasts by withanone extracted from ashwagandha leaves.
AuthorsWidodo N, Shah N, Priyandoko D, Ishii T, Kaul SC, Wadhwa R,
JournalJ Gerontol A Biol Sci Med Sci
PubMed ID19587106
Ashwagandha is an Ayurvedic shrub that forms a common ingredient of health supplements, tonics, and Indian home remedies designed to promote health and quality of life. Though sustained through experience and history, there are only a limited laboratory studies and experimental evidence to its effects. In our efforts to characterize ... More
Amifostine reduces lung vascular permeability via suppression of inflammatory signalling.
Despite an encouraging outcome of antioxidant therapy in animal models of acute lung injury, effective antioxidant agents for clinical application remain to be developed. The present study investigated the effect of pre-treatment with amifostine, a thiol antioxidant compound, on lung endothelial barrier dysfunction induced by Gram-negative bacteria wall-lipopolysaccharide (LPS). Endothelial ... More
Neutrophil stress and apoptosis underlie myeloid dysfunction in glycogen storage disease type Ib.
AuthorsKim SY, Jun HS, Mead PA, Mansfield BC, Chou JY,
JournalBlood
PubMed ID18420828
Glycogen storage disease type Ib (GSD-Ib) is caused by a deficiency in the glucose-6-phosphate (G6P) transporter (G6PT) that works with a liver/kidney/intestine-restricted glucose-6-phosphatase-alpha (G6Pase-alpha) to maintain glucose homeostasis between meals. Clinically, GSD-Ib patients manifest disturbed glucose homeostasis and neutrophil dysfunctions but the cause of the latter is unclear. Neutrophils express ... More
Genome-wide analysis reveals mechanisms modulating autophagy in normal brain aging and in Alzheimer's disease.
AuthorsLipinski MM, Zheng B, Lu T, Yan Z, Py BF, Ng A, Xavier RJ, Li C, Yankner BA, Scherzer CR, Yuan J,
JournalProc Natl Acad Sci U S A
PubMed ID20660724
Dysregulation of autophagy, a cellular catabolic mechanism essential for degradation of misfolded proteins, has been implicated in multiple neurodegenerative diseases. However, the mechanisms that lead to the autophagy dysfunction are still not clear. Based on the results of a genome-wide screen, we show that reactive oxygen species (ROS) serve as ... More
DNA repair is limiting for haematopoietic stem cells during ageing.
AuthorsNijnik A, Woodbine L, Marchetti C, Dawson S, Lambe T, Liu C, Rodrigues NP, Crockford TL, Cabuy E, Vindigni A, Enver T, Bell JI, Slijepcevic P, Goodnow CC, Jeggo PA, Cornall RJ
JournalNature
PubMed ID17554302
Accumulation of DNA damage leading to adult stem cell exhaustion has been proposed to be a principal mechanism of ageing. Here we address this question by taking advantage of the highly specific role of DNA ligase IV in the repair of DNA double-strand breaks by non-homologous end-joining, and by the ... More
Autophagic programmed cell death by selective catalase degradation.
AuthorsYu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E, Baehrecke EH, Lenardo M
JournalProc Natl Acad Sci U S A
PubMed ID16547133
Autophagy plays a central role in regulating important cellular functions such as cell survival during starvation and control of infectious pathogens. Recently, it has been shown that autophagy can induce cells to die; however, the mechanism of the autophagic cell death program is unclear. We now show that caspase inhibition ... More
Characterization of the potent neuroprotective properties of the natural vitamin E alpha-tocotrienol.
AuthorsKhanna S, Roy S, Parinandi NL, Maurer M, Sen CK
JournalJ Neurochem
PubMed ID16923160
The natural vitamin E tocotrienols possess properties not shared by tocopherols. Nanomolar alpha-tocotrienol, not alpha-tocopherol, is potently neuroprotective. On a concentration basis, this finding represents the most potent of all biological functions exhibited by any natural vitamin E molecule. We sought to dissect the antioxidant-independent and -dependent neuroprotective properties of ... More
The longevity SNP rs2802292 uncovered: HSF1 activates stress-dependent expression of FOXO3 through an intronic enhancer.
Authors
JournalNucleic Acids Res
PubMed ID29733381
TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.
Authors
JournalNat Commun
PubMed ID34108482
RalA regulates vascular endothelial growth factor-C (VEGF-C) synthesis in prostate cancer cells during androgen ablation.
Authors
JournalOncogene
PubMed ID16964283
Short-chain fatty acids induced autophagy serves as an adaptive strategy for retarding mitochondria-mediated apoptotic cell death.
Authors
JournalCell Death Differ
PubMed ID20930850
LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy.
Authors
JournalAutophagy
PubMed ID22874552
Enhanced expression of nicotinamide nucleotide transhydrogenase (NNT) and its role in a human T cell line continuously exposed to asbestos.
Authors
JournalEnviron Int
PubMed ID32187573
Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis.
Authors
JournalAutophagy
PubMed ID26391655
Cocaine-mediated microglial activation involves the ER stress-autophagy axis.
Authors
JournalAutophagy
PubMed ID26043790
An approach to the photocatalytic mechanism in the TiO2-nanomaterials microorganism interface for the control of infectious processes.
Authors
JournalAppl Catal B
PubMed ID32292243
Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for animal nociception.