NKT cells are critical to initiate an inflammatory response after Pseudomonas aeruginosa ocular infection in susceptible mice.
AuthorsHazlett LD, Li Q, Liu J, McClellan S, Du W, Barrett RP
JournalJ Immunol
PubMed ID17617607
'CD4(+) T cells produce IFN-gamma contributing to corneal perforation in C57BL/6 (B6) mice after Pseudomonas aeruginosa infection. To determine the role of NK and NKT cells, infected corneas of B6 mice were dual immunolabeled. Initially, more NKT than NK cells were detected, but as disease progressed, NK cells increased, while ... More
Identification of STAT3 as a substrate of receptor protein tyrosine phosphatase T.
AuthorsZhang X, Guo A, Yu J, Possemato A, Chen Y, Zheng W, Polakiewicz RD, Kinzler KW, Vogelstein B, Velculescu VE, Wang ZJ,
JournalProc Natl Acad Sci U S A
PubMed ID17360477
'Protein tyrosine phosphatase (PTP) receptor T (PTPRT) is the most frequently mutated PTP in human cancers. However, the cell signaling pathways regulated by PTPRT have not yet been elucidated. Here, we report identification of signal transducer and activator of transcription 3 (STAT3) as a substrate of PTPRT. Phosphorylation of a ... More
Displacement of SERCA from SR lipid caveolae-related domains by Bcl-2: a possible mechanism for SERCA inactivation.
AuthorsDremina ES, Sharov VS, Schöneich C
JournalBiochemistry
PubMed ID16388593
'Bcl-2 exerts its anti-apoptotic effect in part through the regulation of Ca2+ homeostasis at the level of the endoplasmic reticulum. Earlier, we demonstrated that a truncated form of Bcl-2, Bcl-2delta21, interacts with and destabilizes the skeletal muscle sarco/endoplasmic reticulum Ca-ATPase (SERCA) [Dremina, E. S., Sharov, V. S., Kumar, K., Zaidi, ... More
Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.
AuthorsCai C, Omwancha J, Hsieh CL, Shemshedini L
JournalProstate Cancer Prostatic Dis
PubMed ID17003774
'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. ... More
Constitutive interferon-inducible protein 16-inflammasome activation during Epstein-Barr virus latency I, II, and III in B and epithelial cells.
'Epstein-Barr virus (EBV), etiologically linked with human B-cell malignancies and nasopharyngeal carcinoma (NPC), establishes three types of latency that facilitate its episomal genome persistence and evasion of host immune responses. The innate inflammasome responses recognize the pathogen-associated molecular patterns which lead into the association of a cytoplasmic sensor such as ... More
T cell costimulation with anti-CD137 monoclonal antibodies is mediated by K63-polyubiquitin-dependent signals from endosomes.
AuthorsMartinez-Forero I, Azpilikueta A, Bolaños-Mateo E, Nistal-Villan E, Palazon A, Teijeira A, Perez-Chacon G, Morales-Kastresana A, Murillo O, Jure-Kunkel M, Zapata JM, Melero I,
Journal
PubMed ID23690480
'Agonist anti-CD137 (4-1BB) mAbs enhance CD8-mediated antitumor immunity. Agonist anti-human CD137 mAbs binding to four distinct epitopes on the CD137 glycoprotein costimulated T cell activation irrespective of the engaged epitope or its interference with CD137L binding. CD137 perturbation with all these agonist mAbs resulted in Ag and Ab internalization toward ... More
Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.
AuthorsPenna G, Fibbi B, Amuchastegui S, Cossetti C, Aquilano F, Laverny G, Gacci M, Crescioli C, Maggi M, Adorini L,
JournalJ Immunol
PubMed ID19299703
'Benign prostatic hyperplasia (BPH), a highly prevalent prostatic condition, could involve an inflammatory component in disease pathogenesis. In this study, we show that human stromal prostate cells obtained from BPH tissue can actively contribute to the inflammatory process by secreting proinflammatory cytokines as well as chemokines able to recruit lymphomonuclear ... More
Expression of the homeobox gene Pitx2 in neural crest is required for optic stalk and ocular anterior segment development.
AuthorsEvans AL, Gage PJ,
JournalHum Mol Genet
PubMed ID16203745
'Heterozygous mutations in the homeobox gene, PITX2, result in ocular anterior segment defects and a high incidence of early-onset glaucoma. Pitx2 is expressed in both the neural crest and the mesoderm-derived precursors of the periocular mesenchyme. Complete loss of function in mice results in agenesis or severe disruption of periocular ... More
Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300.
AuthorsWolf L, Harrison W, Huang J, Xie Q, Xiao N, Sun J, Kong L, Lachke SA, Kuracha MR, Govindarajan V, Brindle PK, Ashery-Padan R, Beebe DC, Overbeek PA, Cvekl A,
Journal
PubMed ID24038357
Lens induction is a classical embryologic model to study cell fate determination. It has been proposed earlier that specific changes in core histone modifications accompany the process of cell fate specification and determination. The lysine acetyltransferases CBP and p300 function as principal enzymes that modify core histones to facilitate specific ... More
Borrelia hermsii acquisition order in superinfected ticks determines transmission efficiency.
AuthorsPolicastro PF, Raffel SJ, Schwan TG,
Journal
PubMed ID23716615
Multilocus sequence typing of Borrelia hermsii isolates reveals its divergence into two major genomic groups (GG), but no differences in transmission efficiency or host pathogenicity are associated with these genotypes. To compare GGI and GGII in the tick-host infection cycle, we first determined if spirochetes from the two groups could ... More
Mechanism of cell death caused by complex I defects in a rat dopaminergic cell line.
AuthorsMarella M, Seo BB, Matsuno-Yagi A, Yagi T,
JournalJ Biol Chem
PubMed ID17581813
Defects in the proton-translocating NADH-quinone oxidoreductase (complex I) of mammalian mitochondria are linked to neurodegenerative disorders. The mechanism leading to cell death elicited by complex I deficiency remains elusive. We have shown that expression of a rotenone-insensitive yeast NADH-quinone oxidoreductase (Ndi1) can rescue mammalian cells from complex I dysfunction. By ... More
Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.
AuthorsDesroches BR, Zhang P, Choi BR, King ME, Maldonado AE, Li W, Rago A, Liu G, Nath N, Hartmann KM, Yang B, Koren G, Morgan JR, Mende U,
JournalAm J Physiol Heart Circ Physiol
PubMed ID22427522
To bridge the gap between two-dimensional cell culture and tissue, various three-dimensional (3-D) cell culture approaches have been developed for the investigation of cardiac myocytes (CMs) and cardiac fibroblasts (CFs). However, several limitations still exist. This study was designed to develop a cardiac 3-D culture model with a scaffold-free technology ... More
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
AuthorsMonier K, Mouradian S, Sullivan KF,
JournalJ Cell Sci
PubMed ID17164288
Confinement of enzymatic reactions to nuclear and chromosomal subdomains regulates functional organization of the nucleus. Aurora-B kinase regulates cell-cycle-dependent phosphorylation of chromosomal substrates through sequential localization to a series of sites on chromosomes and the mitotic spindle. In G2 nuclei, Aurora-B recruitment to heterochromatin restricts histone H3S10 phosphorylation to a ... More
Design of the artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells.
AuthorsNagaoka M, Hagiwara Y, Takemura K, Murakami Y, Li J, Duncan SA, Akaike T,
JournalJ Biol Chem
PubMed ID18614540
Embryonic stem (ES) cells are pluripotent-undifferentiated cells that have a great interest for the investigation of developmental biology. Murine ES cells maintain their pluripotency by the supplementation of the leukemia inhibitory factor (LIF). LIF is reported to act as a matrix-anchored form, and immobilized cytokines are useful to sustain their ... More
Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.
AuthorsFumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,
JournalMol Pharm
PubMed ID19492848
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More
A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2.
AuthorsNunes P, Hasler U, McKee M, Lu HA, Bouley R, Brown D,
JournalAm J Physiol Cell Physiol
PubMed ID18799651
Vasopressin (VP)-induced exocytosis was dissected in native and aquaporin-2 (AQP2)-expressing renal LLC-PK(1) cells by a fluorimetric exocytosis assay based on soluble secreted yellow fluorescent protein (ssYFP). YFP was targeted to the secretory pathway by addition of an 18-amino acid signal peptide from hen egg white lysozyme. Immunofluorescence labeling, together with ... More
Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel Incorporation of BrdU and EdU Into Newly Synthesized mtDNA In Vitro.
AuthorsLentz SI, Edwards JL, Backus C, McLean LL, Haines KM, Feldman EL,
JournalJ Histochem Cytochem
PubMed ID19875847
Mitochondria are key regulators of cellular energy and are the focus of a large number of studies examining the regulation of mitochondrial dynamics and biogenesis in healthy and diseased conditions. One approach for monitoring mitochondrial biogenesis is to measure the rate of mitochondrial DNA (mtDNA) replication. We developed a sensitive ... More
Prohormone convertase 1/3 is essential for processing of the glucose-dependent insulinotropic polypeptide precursor.
The physiology of the incretin hormones, glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), and their role in type 2 diabetes currently attract great interest. Recently we reported an essential role for prohormone convertase (PC) 1/3 in the cleavage of intestinal proglucagon, resulting in formation of GLP-1, as demonstrated ... More
TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death.
Authors
JournalNat Commun
PubMed ID24284943
Small RNA pathways in the nematode Ascaris in the absence of piRNAs.
Authors
JournalNat Commun
PubMed ID35149688
p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors.
Authors
JournalCancer Cell
PubMed ID30581152
iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture.
Authors
JournalNat Commun
PubMed ID30967549
Interferon-γ induces tumor resistance to anti-PD-1 immunotherapy by promoting YAP phase separation.
Authors
JournalMol Cell
PubMed ID33606996
Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls Adipogenesis.
Authors
JournalMol Cell
PubMed ID32822587
Direct supercritical angle localization microscopy for nanometer 3D superresolution.
Authors
JournalNat Commun
PubMed ID33608524
3D particle averaging and detection of macromolecular symmetry in localization microscopy.
Authors
JournalNat Commun
PubMed ID33990554
A mechanically-induced colon cancer cell population shows increased metastatic potential.
Authors
JournalMol Cancer
PubMed ID24884630
Z-form extracellular DNA is a structural component of the bacterial biofilm matrix.