GeneJET 凝胶回收试剂盒

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We have updated the storage temperature of the purification columns for this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

Thermo Scientific™

GeneJET 凝胶回收试剂盒

Name: GeneJET 凝胶回收试剂盒
SKU: K0692

Thermo Scientific GeneJET 凝胶回收试剂盒设计用于从 TAE 或 TBE 缓冲液运行的标准或低熔点琼脂糖凝胶中快速高效地纯化 DNA 片段了解更多信息

更改视图

货号	数量
K0692	250 次反应
K0691	50 次制备

2 选项

货号 K0692

价格（CNY)

数量:

250 次反应

Thermo Scientific GeneJET 凝胶回收试剂盒设计用于从 TAE 或 TBE 缓冲液运行的标准或低熔点琼脂糖凝胶中快速高效地纯化 DNA 片段。

该试剂盒采用便捷离心柱形式的专有硅胶膜技术。该试剂盒可用于纯化大小为 25 bp 至 20 kb 的 DNA 片段，回收率高达 95%。每个 GeneJET 纯化柱的 DNA 结合能力高达 25 μg，可处理的琼脂糖凝胶高达 1 g。整个过程只需 15 分钟，分离的 DNA 即可用于所有常见的下游应用，包括连接、限制性酶切、PCR、测序和标记。

产品优势

•高效：高达 95% 回收率，范围为 100 bp 至 10 kb
• 快速：程序仅需 15 分钟
• 便捷：离心柱带盖且装有收集管

应用

•快速高效提取高纯度的 DNA 片段，是所有常规分子生物学程序的理想选择，包括：
•FastDigest 或常规限制性酶切
•自动荧光或放射测序
• PCR
• 原位杂交
•标记
•克隆

仅供科研使用。不可用于诊断程序。

规格

色谱柱类型离心柱

洗脱体积30 至 100 μL

最终产品类型片段文库、PCR 扩增子、cDNA 文库、寡核苷酸 (DNA)、cDNA

适用于（应用）实时荧光定量 PCR (qPCR)、克隆、Southern 印迹、测序、PCR

高通量能力不兼容高通量应用（手动）

数量250 次反应

样品类型凝胶

运输条件室温

原始材料量≤1 g

测试时间15 分钟

产量25 μg (Binding capacity)

分离技术离心柱

Unit SizeEach

内容与储存

For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

Can I use GeneJET Gel Extraction Kit for PCR clean-up?

The GeneJET Gel Extraction Kit is designed for gel extraction but can also be used for PCR clean-up by following the protocol from the GeneJET PCR Purification Kit. The components in the GeneJET Gel Extraction Kit and GeneJET PCR Purification Kit are very similar and can be used interchangeably. The exact composition of solutions in the kits is proprietary.

For a 3-in-1 kit for purification of PCR products from enzymatic reaction mixtures and agarose gels, we recommend using the GeneJET Gel Extraction and DNA Cleanup Micro Kit (Cat. Nos. K0831/2).

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

View all GeneJET 凝胶回收试剂盒 FAQs