GeneJET RNA 纯化试剂盒
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We have updated the storage temperature of the purification columns in this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
GeneJET RNA 纯化试剂盒
Thermo Scientific™

GeneJET RNA 纯化试剂盒

Thermo Scientific GeneJET RNA 纯化试剂盒是一种简单、高效的系统,用于纯化来自哺乳动物培养细胞、组织、人血细胞、细菌和酵母菌的总 RNA了解更多信息
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货号数量
K0732250 次制备
K073150 次制备
货号 K0732
价格(CNY)
-
数量:
250 次制备
Thermo Scientific GeneJET RNA 纯化试剂盒是一种简单、高效的系统,用于纯化来自哺乳动物培养细胞、组织、人血细胞、细菌和酵母菌的总 RNA。

该试剂盒采用便捷离心柱形式的硅胶膜技术,使用方便,无需使用繁琐的氯化铯梯度、酒精沉淀或有毒的苯酚-氯仿提取。

长度超过 200 个核甘酸的 RNA 分子,可通过 GeneJET RNA 纯化试剂盒在裂解步骤后 15 分钟内完成分离。高质量纯化 RNA 可用于多种下游应用,包括 RT-PCR、RT-qPCR、Northern 印迹和其他 RNA 分析。

产品优势

通用 – 可用于多种来源的细胞和组织样品:哺乳动物血液、哺乳动物细胞培养物、哺乳动物组织、昆虫、酵母菌和细菌
高效 – 总 RNA 得率较高。
快速 – 裂解步骤后只需 15 分钟即可完成
高纯度 – A260/280 比 > 1.9
便利 – 离心柱带盖并装配有收集管

应用

• 适用于所有常规分子生物学程序的快速高纯度 RNA 提取,包括:
•RT-PCR
• RT-qPCR
• Northern 印迹
• 核酸酶保护测定。包括蛋白酶 K、裂解缓冲液、洗涤缓冲液 1(浓缩液)、洗涤缓冲液 2(浓缩液)、无核酸酶水、GeneJET RNA 纯化柱(预装有收集管)、收集管 (2 mL)、收集管 (1.5 mL)、详细方案
仅供科研使用。不可用于诊断程序。
规格
洗脱体积50–100 μL
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)RT-PCR、qPCR、cDNA 文库构建、NGS、微阵列分析、印迹杂交、Northern 印迹、体外翻译、核酸酶保护测定、核酸标记
高通量能力不兼容高通量应用(手动)
纯化时间15分钟(样本均质后)
数量250 次制备
规格小型
运输条件室温
原始材料量细菌:≤109个细胞
血液:0.5 mL
细胞:≤107
组织和昆虫:30 mg
酵母:≤4 x 108个细胞
酶促反应:≤100 μL
产量高达 160 μg
分离技术硅胶离心柱
样品类型细菌、血液、细胞、组织、酵母菌、昆虫,酶促反应(DNase 消化、RNA 标记/纯化制备、IVT), 血液, 细胞, 组织, 酵母菌, Insects, Enzymatic Reactions
Unit SizeEach
内容与储存
• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 200 mL Lysis Buffer; room temperature
• 200 mL Wash Buffer 1 (concentrated); room temperature
• 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Nuclease-free Water; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.