GeneJET RNA Purification Kit, 250 preps - FAQs

查看更多产品信息 GeneJET RNA Purification Kit - FAQs (K0732, K0731)

7 个常见问题解答

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

Does the GeneJET Stabilized and Fresh Whole Blood RNA Kit isolate miRNA?

This kit is not suitable for purification of small RNAs.

Do the GeneJET RNA purification kits remove genomic DNA?

These kits will remove the majority of genomic DNA, however trace amounts may still remain. Therefore, for applications that are very sensitive to genomic DNA, we recommend you treat the purified RNA sample with DNase I (Cat. No. EN0521), RapidOut DNA Removal Kit (Cat. No. K2981), or dsDNase (Cat. No. EN0771).