The protein phosphatases of Synechocystis sp. strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro.
AuthorsLi R,Potters MB,Shi L,Kennelly PJ
JournalJournal of bacteriology
PubMed ID16109928
The open reading frames (ORFs) encoding two potential protein-serine/threonine phosphatases from the cyanobacterium Synechocystis sp. strain PCC 6803 were cloned and their protein products expressed in Escherichia coli cells. The product of ORF sll1033, SynPPM3, is a homologue of the PPM family of protein-serine/threonine phosphatases found in all eukaryotes as ... More
A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei.
AuthorsSchimanski B, Brandenburg J, Nguyen TN, Caimano MJ, Günzl A,
JournalNucleic Acids Res
PubMed ID16554554
'The lack of general class II transcription factors was a hallmark of the genomic sequences of the human parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania major. However, the recent identification of TFIIA as part of a protein complex essential for RNA polymerase II-mediated transcription of SLRNA genes, which encode the ... More
A GPI-linked carbonic anhydrase expressed in the larval mosquito midgut.
AuthorsSeron TJ, Hill J, Linser PJ,
JournalJ Exp Biol
PubMed ID15579552
'We have previously described the first cloning and partial characterization of carbonic anhydrase from larval Aedes aegypti mosquitoes. Larval mosquitoes utilize an alkaline digestive environment in the lumen of their anterior midgut, and we have also demonstrated a critical link between alkalization of the gut and carbonic anhydrase(s). In this ... More
Biophysical characterization of the interaction domains and mapping of the contact residues in the XPF-ERCC1 complex.
AuthorsChoi YJ, Ryu KS, Ko YM, Chae YK, Pelton JG, Wemmer DE, Choi BS,
JournalJ Biol Chem
PubMed ID15932882
'XPF and ERCC1 exist as a heterodimer to be stable and active in cells andatalyze DNA cleavage on the 5''-side of a lesion during nucleotide excision repair. To characterize the specific interaction between XPF and ERCC1, we expressed the human ERCC1 binding domain of XPF (XPF-EB) and the XPF binding ... More
ADAMTS13 substrate recognition of von Willebrand factor A2 domain.
AuthorsZanardelli S, Crawley JT, Chion CK, Lam JK, Preston RJ, Lane DA,
JournalJ Biol Chem
PubMed ID16221672
'ADAMTS13 controls the multimeric size of circulating von Willebrand factor (VWF) by cleaving the Tyr1605-Met1606 bond in theA2 domain. To examine substrate recognition, we expressed in bacteria and purified three A2 (VWF76-(1593-1668), VWF115-(1554-1668), VWFA2-(1473-1668)) and one A2-A3 (VWF115-A3-(1554-1874)) domain fragments. Using high pressure liquid chromatography analysis, the initial rates of ... More
Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system.
AuthorsStudier FW
JournalJ Mol Biol
PubMed ID2023259
'Bacteriophage T7 lysozyme, a natural inhibitor of T7 RNA polymerase, can reduce basal activity from an inducible gene for T7 RNA polymerase and allow relatively toxic genes to be established in the same cell under control of a T7 promoter. Low levels of T7 lysozyme supplied by plasmids pLysS or ... More
Expression of a viral polymerase-bound host factor turns human cell lines permissive to a plant- and insect-infecting virus.
Authorsde Medeiros RB, Figueiredo J, Resende Rde O, De Avila AC,
JournalProc Natl Acad Sci U S A
PubMed ID15657123
'Tospoviruses are the only plant-infecting members of the Bunyaviridae family of ambisense ssRNA viruses. Tomato spotted wilt tospovirus (TSWV), the type-member, also causes mild infection on its main insect vector, Frankliniella occidentalis. Herein, we identified an F. occidentalis putative transcription factor (FoTF) that binds to the TSWV RNA-dependent RNA polymerase ... More
2-Haloacrylate reductase, a novel enzyme of the medium chain dehydrogenase/reductase superfamily that catalyzes the reduction of a carbon-carbon double bond of unsaturated organohalogen compounds.
AuthorsKurata A, Kurihara T, Kamachi H, Esaki N,
JournalJ Biol Chem
PubMed ID15781461
A soil bacterium, Burkholderia sp. WS, grows on 2-chloroacrylate as the sole carbon source. To identify the enzymes metabolizing 2-chloroacrylate, we carried out comparative two-dimensional gel electrophoresis of the proteins from 2-chloroacrylate- and lactate-grown bacterial cells. As a result, we found that a protein named CAA43 was inducibly synthesized when ... More
Brucella abortus virB12 is expressed during infection but is not an essential component of the type IV secretion system.
The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, orf12 (virB12) and orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether orf12 (virB12) encodes ... More
The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization.
AuthorsThomas J, Cronan JE,
JournalJ Biol Chem
PubMed ID16107329
The acyl carrier proteins (ACPs) of fatty acid synthesis are functional only when modified by attachment of the prosthetic group, 4'-phosphopantetheine (4'-PP), which is transferred from CoA to the hydroxyl group of a specific serine residue. Almost 40 years ago Vagelos and Larrabee reported an enzyme from Escherichia coli that ... More
Transcriptional regulation and characterization of a novel beta-fructofuranosidase-encoding gene from Bifidobacterium breve UCC2003.
AuthorsRyan SM, Fitzgerald GF, van Sinderen D,
JournalAppl Environ Microbiol
PubMed ID16000751
An operon involved in fructooligosaccharide breakdown was identified in the genome of Bifidobacterium breve UCC2003. This 2.6-kb transcriptional unit was comprised of three genes that encoded a putative permease, a conserved hypothetical protein, and a beta-fructofuranosidase. Active transcription of the operon was observed when B. breve UCC2003 was grown on ... More
Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression.
AuthorsGerbasi VR, Weaver CM, Hill S, Friedman DB, Link AJ,
JournalMol Cell Biol
PubMed ID15340087
Translation of mRNA into protein is a fundamental step in eukaryotic gene expression requiring the large (60S) and small (40S) ribosome subunits and associated proteins. By modern proteomic approaches, we previously identified a novel 40S-associated protein named Asc1p in budding yeast and RACK1 in mammals. The goals of this study ... More
Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response.
AuthorsDjalilian AR, McGaughey D, Patel S, Seo EY, Yang C, Cheng J, Tomic M, Sinha S, Ishida-Yamamoto A, Segre JA,
JournalJ Clin Invest
PubMed ID16628254
Inflammatory skin disorders result in significant epidermal changes, including keratinocyte hyperproliferation, incomplete differentiation, and impaired barrier. Here we test whether, conversely, an impaired epidermal barrier can promote an inflammatory response. Mice lacking the transcription factor Kruppel-like factor 4 (Klf4) have a severe defect in epidermal barrier acquisition. Transcription profiling of ... More
Ezrin controls the macromolecular complexes formed between an adapter protein Na+/H+ exchanger regulatory factor and the cystic fibrosis transmembrane conductance regulator.
AuthorsLi J, Dai Z, Jana D, Callaway DJ, Bu Z,
JournalJ Biol Chem
PubMed ID16129695
Na(+)/H(+) exchanger regulatory factor (NHERF) is an adapter protein that is responsible for organizing a number of cell receptors and channels. NHERF contains two amino-terminal PDZ (postsynaptic density 95/disk-large/zonula occluden-1) domains that bind to the cytoplasmic domains of a number of membrane channels or receptors. The carboxyl terminus of NHERF ... More