Differential gene expression between developing queens and workers in the honey bee, Apis mellifera [see comments]
AuthorsEvans JD, Wheeler DE
JournalProc Natl Acad Sci U S A
PubMed ID10318926
'Many insects show polyphenisms, or alternative morphologies, which are based on differential gene expression rather than genetic polymorphism. Queens and workers are alternative forms of the adult female honey bee and represent one of the best known examples of insect polyphenism. Hormonal regulation of caste determination in honey bees has ... More
An imprinted, mammalian bicistronic transcript encodes two independent proteins.
AuthorsGray TA, Saitoh S, Nicholls RD
JournalProc Natl Acad Sci U S A
PubMed ID10318933
Polycistronic transcripts are common in prokaryotes but rare in eukaryotes. Phylogenetic analysis of the SNRPN (SmN) mRNA in five eutherian mammals reveals a second highly conserved coding sequence, termed SNURF (SNRPN upstream reading frame). The vast majority of nucleotide substitutions in SNURF occur in the wobble codon position, providing strong ... More
PCR-mediated recombination: a general method applied to construct chimeric infectious molecular clones of plasma-derived HIV-1 RNA.
AuthorsFang G, Weiser B, Visosky A, Moran T, Burger H
JournalNature Medicine
PubMed ID9930876
A PCR-based approach was developed that provides a powerful tool for engineering recombinant molecules without reliance on restriction sites. DNA sequences were first amplified by high-fidelity PCR using Pfu polymerase; they were then used both as 'megaprimers' and templates in subsequent asymmetric long PCR amplifications to form chimeric clones. To ... More
Sequence evolution and copy number of Ty1-copia retrotransposons in diverse plant genomes.
Authors Navarro-Quezada Aura; Schoen Daniel J;
JournalProc Natl Acad Sci U S A
PubMed ID11752395
Sequence evolution of the reverse transcriptase (RT) gene in retrotransposons belonging to the Ty1-copia class was studied in 11 plant species. Phylogenetic reconstruction of the evolutionary history of RT sequences indicated a strong pattern of purifying selection, manifested as high ratios of third to first plus second codon position substitutions, ... More
Immunogene therapy of tumors with vaccine based on Xenopus homologous vascular endothelial growth factor as a model antigen.
Authors Wei Y Q; Huang M J; Yang L; Zhao X; Tian L; Lu Y; Shu J M; Lu C J; Niu T; Kang B; Mao Y Q; Liu F; Wen Y J; Lei S; Luo F; Zhou L Q; Peng F; Jiang Y; Liu J Y; Zhou H; Wang Q R; He Q M; Xiao F; Lou Y Y; Xie X J; Li Q; Wu Y; Ding Z Y; Hu B; Hu M; Zhang W;
JournalProc Natl Acad Sci U S A
PubMed ID11553767
Overcoming immune tolerance of the growth factors associated with tumor growth should be a useful approach to cancer therapy by active immunity. We used vascular endothelial growth factor (VEGF) as a model antigen to explore the feasibility of the immunogene tumor therapy with a vaccine based on a single xenogeneic ... More