Expression of human NAA11 (ARD1B) gene is tissue-specific and is regulated by DNA methylation.
AuthorsPang AL, Clark J, Chan WY, Rennert OM
JournalEpigenetics
PubMed ID22048246
'NAA10 gene encodes the catalytic subunit of N(alpha)-acetyltransferase NatA that catalyzes the acetylation of the N-termini of many eukaryotic proteins. A homologous gene called NAA11 is also present in mammalian cells. hNaa10p and hNaa11p are reported to be co-expressed in human cell cultures. In mouse tissues, however, Naa11 transcripts can ... More
Construction and testing of engineered zinc-finger proteins for sequence-specific modification of mtDNA.
AuthorsMinczuk M, Kolasinska-Zwierz P, Murphy MP, Papworth MA
JournalNat Protoc
PubMed ID20134433
Engineered zinc-finger proteins (ZFPs) are hybrid proteins developed to direct various effector domains (EDs) of choice to predetermined DNA sequences. They are used to alter gene expression and to modify DNA in a sequence-specific manner in vivo and in vitro. Until now, ZFPs have mostly been used to target DNA ... More
Quantification of adenosine-to-inosine editing of microRNAs using a conventional method.
AuthorsKawahara Y
JournalNat Protoc
PubMed ID22743833
In this protocol, I describe a method for measuring the frequency of adenosine-to-inosine RNA editing of primary, precursor and mature forms of specific microRNAs (miRNAs) derived from the same source. The procedure involves reverse transcription (RT)-PCR amplification of regions containing the editing sites followed by subcloning of the PCR products ... More
Glycosylation of wall teichoic acid in Staphylococcus aureus by TarM.
AuthorsXia G, Maier L, Sanchez-Carballo P, Li M, Otto M, Holst O, Peschel A
JournalJ Biol Chem
PubMed ID20185825
Wall teichoic acid (WTA) glycopolymers are major constituents of cell envelopes in Staphylococcus aureus and related gram-positive bacteria with important roles in cell wall maintenance, susceptibility to antimicrobial molecules, biofilm formation, and host interaction. Most S. aureus strains express polyribitol phosphate WTA substituted with D-alanine and N-acetylglucosamine (GlcNAc). WTA sugar ... More