PureLink™ PCR Purification Kit
We have updated the storage temperature of the purification columns for this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
PureLink™ PCR Purification Kit
Invitrogen™

PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
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Catalog NumberQuantity
K310002250 Preps
K31000150 Preps
Catalog number K310002
Price (CNY)
6,481.00
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Ends: 31-Dec-2025
8,099.00
Save 1,618.00 (20%)
Each
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Quantity:
250 Preps
Price (CNY)
6,481.00
Online Exclusive
Ends: 31-Dec-2025
8,099.00
Save 1,618.00 (20%)
Each
Add to cart
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:

  • 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
  • No need for tedious sample pH adjustments necessary with other kits
  • Convenient and rapid protocol, completed in less than 10 minutes
  • Optional size-selection buffers included for selectively purifying 300–600 bp fragments

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume Max50 μL
Final Product TypePCR Amplicon
FormatKit
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Isolation TechnologySilica Spin Column
Purification Time15 min.
Quantity250 Preps
Run Time10 min.
Sample TypePCR Products
Shipping ConditionRoom Temperature
Starting Material Volume≤100 μL PCR product, or ≤40 μg dsDNA
Yield40 μg (Binding capacity)
For Use With (Application)Standard PCR, DNA Sequencing, Restriction Enzyme Digestion, Nucleic Acid Labeling, Cloning
Unit SizeEach
Contents & Storage
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

Frequently asked questions (FAQs)

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.