PureLink™ PCR Purification Kit, 250 preps - FAQs

View additional product information for PureLink™ PCR Purification Kit - FAQs (K310002, K310001)

6 product FAQs found

我能否使用PureLink PCR纯化试剂盒来纯化限制性内切酶酶切后的质粒DNA？

是的，您可以使用我们的试剂盒来纯化限制性内切酶酶切后的质粒DNA。

我能否使用PureLink PCR纯化试剂盒来开展凝胶纯化实验？

PureLink PCR纯化试剂盒与PureLink 凝胶纯化试剂盒两者间的缓冲液和操作步骤均不同。因此，您可以换用离心柱，但须注意使用正确的缓冲液和实验方案。

应用PureLink PCR纯化试剂盒（PureLink PCR Clean-Up Kit）可纯化的DNA片段大小范围是多少？

纯化的片段大小介于100 bp与12 kb之间。我们的试剂盒附带两款缓冲液，结合缓冲液（B2）与结合缓冲液HC（B3）。结合缓冲液HC（B3）能够去除引物二聚体和小于300bp的扩增失败的短PCR产物。

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.