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引用和文献 (7)
Invitrogen™
pYES2.1 TOPO™ TA 酵母菌表达试剂盒
pYES2.1 TOPO TA 表达试剂盒将 Taq 扩增 PCR 产物直接克隆到酿酒酵母表达载体中。该试剂盒使用线性化拓扑异构酶 I了解更多信息
| 货号 | 数量 |
|---|---|
| K415001 | 20 reactions |
货号 K415001
价格（CNY)
13,173.40
飞享价
Ends: 31-Dec-2025
15,730.00共减 2,556.60 (16%)
Each
数量:
20 reactions
价格（CNY)
13,173.40
飞享价
Ends: 31-Dec-2025
15,730.00共减 2,556.60 (16%)
Each
pYES2.1 TOPO TA 表达试剂盒将 Taq 扩增 PCR 产物直接克隆到酿酒酵母表达载体中。该试剂盒使用线性化拓扑异构酶 I 活化 pYES2.1/V5-His-TOPO ¤ 载体在实验台上进行 5 分钟克隆，并产生 > 85% 重组。pYES2.1/V5- His-TOPO™ 载体特征：
•2µ 个用于高拷贝维持基因的复制起始点，用于在经济基本培养基上进行营养缺陷型选择
•用于高效检测和纯化的 C 端 V5 抗原决定簇和多聚组氨酸 (6xHis) 标签
•2µ 个用于高拷贝维持基因的复制起始点，用于在经济基本培养基上进行营养缺陷型选择
•用于高效检测和纯化的 C 端 V5 抗原决定簇和多聚组氨酸 (6xHis) 标签
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌氨苄青霉素 (AmpR)
产品类型TA 酵母表达试剂盒
数量20 reactions
载体pYES、TOPO-TA 克隆载体
克隆方法TOPO™-TA
产品线TOPO、YES, YES
促进剂GAL1
蛋白标记His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
Unit SizeEach
内容与储存
每个 pYES2.1 TOPO™ TA 表达试剂盒包含两个盒。pYES2.1 TOPO™ TA 盒包含连接所需的所有试剂，包括 200 ng 拓扑异构酶 I 活化 pYES2.1/V5-His-TOPO™ 载体、无菌水、dNTP、10X PCR 缓冲液、盐溶液、对照模板和引物、用于测序或 PCR 筛选的 GAL1 正向和 V5 C 端反向引物以及表达对照质粒。储存在 -20°C 下。One Shot™ 盒包含转化所需的所有试剂，包括 21 份 50 µL 化学感受态 TOP10F´大肠杆菌、S.O.C. 培养基和超螺旋质粒对照 pUC19 的等份试液。储存在 -80°C 下。妥善储存时，可保证所有组分在指示温度下稳定储存 6 个月。
常见问题解答 (FAQ)
我可将感受态细胞存储在液氮中吗？
我应该如何存储我的感受态E. Coli？
Pichia pastoris和S. cerevisiae的培养基有哪些不同种类？
Schizosaccharomyces pombe能否识别Saccharomyces cerevisiae的α-因子分泌信号？
你们是否提供TOPO适应型酵母表达载体？
引用和文献 (7)
引用和文献
Abstract
Identification of a family of animal sphingomyelin synthases.
Journal:EMBO J
PubMed ID:14685263
'Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product. This reaction is catalysed by SM synthase, an enzyme whose biological potential can be judged from the roles of diacylglycerol and ceramide as
Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability.
Journal:Mol Cell Biol
PubMed ID:10373538
'In Saccharomyces cerevisiae, the heteromeric kinase complex Cdc7p-Dbf4p plays a pivotal role at replication origins in triggering the initiation of DNA replication during the S phase. We have assayed the kinase activity of endogenous levels of Cdc7p kinase by using a likely physiological target, Mcm2p, as a substrate. Using this
Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae.
Journal:J Biol Chem
PubMed ID:11940600
'All members of the regulator of G protein signaling (RGS) family contain a conserved core domain that can accelerate G protein GTPase activity. The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating. In addition, some RGS proteins contain an N-terminal domain of unknown function. Here
Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling.
Journal:Nature Biotechnology
PubMed ID:10471929
We describe genetic screens in Saccharomyces cerevisiae designed to identify mammalian nonreceptor modulators of G-protein signaling pathways. Strains lacking a pheromone-responsive G-protein coupled receptor and expressing a mammalian-yeast Galpha hybrid protein were made conditional for growth upon either pheromone pathway activation (activator screen) or pheromone pathway inactivation (inhibitor screen). Mammalian
Pheromone-dependent Ubiquitination of the Mitogen-activated Protein Kinase Kinase Ste7.
Journal:J Biol Chem
PubMed ID:11864977
Many cell signaling pathways are regulated by phosphorylation, ubiquitination, and degradation of constituent proteins. As with phosphorylation, protein ubiquitination can be reversed, through the action of ubiquitin-specific processing proteases (UBPs). Here we have analyzed 15 UBP disruption mutants in the yeast Saccharomyces cerevisiae and identified one (ubp3Delta) that acts specifically