Identification of a family of animal sphingomyelin synthases.
AuthorsHuitema K, Van Den Dikkenberg J, Brouwers JF, Holthuis JC,
JournalEMBO J
PubMed ID14685263
'Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product. This reaction is catalysed by SM synthase, an enzyme whose biological potential can be judged from the roles of diacylglycerol and ceramide as ... More
Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability.
AuthorsOshiro G, Owens JC, Shellman Y, Sclafani RA, Li JJ
JournalMol Cell Biol
PubMed ID10373538
'In Saccharomyces cerevisiae, the heteromeric kinase complex Cdc7p-Dbf4p plays a pivotal role at replication origins in triggering the initiation of DNA replication during the S phase. We have assayed the kinase activity of endogenous levels of Cdc7p kinase by using a likely physiological target, Mcm2p, as a substrate. Using this ... More
Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae.
Authors Burchett Scott A; Flanary Paul; Aston Christopher; Jiang Lixin; Young Kathleen H; Uetz Peter; Fields Stanley; Dohlman Henrik G;
JournalJ Biol Chem
PubMed ID11940600
'All members of the regulator of G protein signaling (RGS) family contain a conserved core domain that can accelerate G protein GTPase activity. The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating. In addition, some RGS proteins contain an N-terminal domain of unknown function. Here ... More
Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling.
AuthorsCismowski MJ, Takesono A, Ma C, Lizano JS, Xie X, Fuernkranz H, Lanier SM, Duzic E
JournalNature Biotechnology
PubMed ID10471929
We describe genetic screens in Saccharomyces cerevisiae designed to identify mammalian nonreceptor modulators of G-protein signaling pathways. Strains lacking a pheromone-responsive G-protein coupled receptor and expressing a mammalian-yeast Galpha hybrid protein were made conditional for growth upon either pheromone pathway activation (activator screen) or pheromone pathway inactivation (inhibitor screen). Mammalian ... More
Pheromone-dependent Ubiquitination of the Mitogen-activated Protein Kinase Kinase Ste7.
Authors Wang Yuqi; Dohlman Henrik G;
JournalJ Biol Chem
PubMed ID11864977
Many cell signaling pathways are regulated by phosphorylation, ubiquitination, and degradation of constituent proteins. As with phosphorylation, protein ubiquitination can be reversed, through the action of ubiquitin-specific processing proteases (UBPs). Here we have analyzed 15 UBP disruption mutants in the yeast Saccharomyces cerevisiae and identified one (ubp3Delta) that acts specifically ... More
Molecular characterization of a mannoprotein with homology to chitin deacetylases that stimulates T cell responses to Cryptococcus neoformans.
Authors Levitz S M; Nong S; Mansour M K; Huang C; Specht C A;
JournalProc Natl Acad Sci U S A
PubMed ID11504924
The fungus Cryptococcus neoformans is a major cause of morbidity and mortality in patients with impaired CD4(+) T cell function, particularly those with AIDS. To identify cryptococcal antigens that could serve as vaccine candidates by stimulating T cell responses, C. neoformans-reactive CD4(+) T cell hybridomas were generated by immunization of ... More
Splitting the two pore domains from TOK1 results in two cationic channels with novel functional properties.
Potassium channels are membrane-spanning proteins with several transmembrane segments and a single pore region where ion conduction takes place (Biggin, P. C., Roosild, T., and Choe, S. (2000) Curr. Opin. Struct. Biol. 4, 456-461; Doyle, D. A., Morais Cabral, J., Pfuetzner, R. A., Kuo, A., Gulbis, J. M., Cohen, S. ... More