Multiple sites of V lambda diversification in cattle.
AuthorsLucier MR, Thompson RE, Waire J, Lin AW, Osborne BA, Goldsby RA
JournalJ Immunol
PubMed ID9820519
'Ig repertoire diversification in cattle was studied in the ileal Peyer''s patch (IPP) follicles of young calvesand in the spleens of late first-trimester bovine fetuses. To investigate follicular diversification, individualIPP follicles were isolated by microdissection; VA diversity was examined by RT-PCR and subsequentcloning and sequencing. When 52 intrafollicular sequences from ... More