Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
AuthorsSong JH, Waataja JJ, Martemyanov KA,
JournalJ Biol Chem
PubMed ID16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
A nuclear export signal within the high mobility group domain regulates the nucleocytoplasmic translocation of SOX9 during sexual determination.
Authors Gasca Stephan; Canizares Joaquin; De Santa Barbara Pascal; Mejean Catherine; Poulat Francis; Berta Philippe; Boizet-Bonhoure Brigitte;
JournalProc Natl Acad Sci U S A
PubMed ID12169669
'In mammals, male sex determination starts when the Y chromosome Sry gene is expressed within the undetermined male gonad. One of the earliest effect of Sry expression is to induce up-regulation of Sox9 gene expression in the developing gonad. SOX9, like SRY, contains a high mobility group domain and is ... More
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.
Authors Laura Richard P; Witt Andrea S; Held Heike A; Gerstner Resi; Deshayes Kurt; Koehler Michael F T; Kosik Kenneth S; Sidhu Sachdev S; Lasky Laurence A;
JournalJ Biol Chem
PubMed ID11821434
'Erbin is a recently described member of the LAP (leucine-rich repeat and PDZ domain) protein family. We used a C-terminally displayed phage peptide library to identify optimal ligands for the Erbin PDZ domain. Phage-selected peptides were type 1 PDZ ligands that bound with high affinity and specificity to the Erbin ... More
Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family.
AuthorsKnoops B, Clippe A, Bogard C, Arsalane K, Wattiez R, Hermans C, Duconseille E, Falmagne P, Bernard A
JournalJ Biol Chem
PubMed ID10521424
Using two-dimensional electrophoresis, we have recently identified in human bronchoalveolar lavage fluid a novel protein, termed B166, with a molecular mass of 17kDa. Here, we report the cloning of human and rat cDNAs encoding B166, which has been renamed AOEB166 for antioxidant enzyme B166. Indeed, the deducedamino acid sequence reveals ... More
Molecular and Functional Interaction of the ATP-binding Cassette Transporter A1 with Fas-associated Death Domain Protein.
ATP-binding cassette transporter A1 (ABCA1) is a major regulator of cellular cholesterol and phospholipid homeostasis. Its function has not been fully characterized and may depend on the association with additional proteins. To identify ABCA1-interacting proteins a human liver yeast two-hybrid library was screened with the 144 C-terminal amino acids of ... More
Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members.
Ubiquitous calpains (mu- and m-calpain) have been repeatedly implicated in apoptosis, but the underlying mechanism(s) remain(s) to be elucidated. We examined ionomycin-induced cell death in LCLC 103H cells, derived from a human large cell lung carcinoma. We detected hallmarks of apoptosis such as membrane blebbing, nuclear condensation, DNA ladder formation, ... More