I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?
Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.
Diversity in tissue expression, substrate binding and SCF complex formation for a lectin family of ubiquitin ligases.
Authors:Glenn KA, Nelson RF, Wen HM, Mallinger AJ, Paulson HL,
Journal:J Biol Chem
PubMed ID:18203720
'Post-translational modification of proteins regulates many cellular processes. Some modifications, including N-linked glycosylation, serve multiple functions. For example, the attachment of N-linked glycans to nascent proteins in the endoplasmic reticulum (ER) facilitates proper folding, while retention of high mannose glycans on misfolded glycoproteins serves as a signal for retrotranslocation and ... More