MagicMedia™ E. coli Expression Medium - FAQs

View additional product information for MagicMedia™ E. coli Expression Medium - FAQs (K6803)

10 product FAQs found

MagicMedia培养基能否使用较低的孵育温度？

可以，使用双温度表达方案已经验证过在18°C和25°C表达。

使用Champion pET载体和BL21 AI菌株时，能否使用MagicMedia培养基进行表达？

很遗憾，MagicMedia培养基不能与阿拉伯糖诱导剂一起工作。MagicMedia培养基需要IPTG诱导系统和完整的Lac操纵子。

什么是MagicMedia大肠杆菌表达培养基？

MagicMedia培养基是一种大肠杆菌表达培养基。它可使蛋白得率提高3-10倍，可用于含有功能性lac操纵子的T7调控型大肠杆菌载体和BL21 (DE3) 菌株，易于操作，并且可省去OD监测/诱导步骤。

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does expression in MagicMedia affect the solubility of proteins when compared to standard LB and IPTG induction?

In most cases, the ratio of soluble to insoluble proteins is not much different when compared to LB and IPTG. However, since more total protein is typically obtained in MagicMedia, one also obtains more total soluble protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does MagicMedia compare to LB-IPTG with regards to expression of toxic proteins?

Toxic protein expression has not been investigated using MagicMedia. However, the Dual Temperature protocol provided in the manual can be tried. This may be a situation where LB/IPTG induction has an advantage since parameters (time, temperature, and IPTG concentration) are easily adjusted for optimal expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Does MagicMedia have options to express and induce at mid-log phase?

No. Protein expression in MagicMedia occurs gradually over an 18-24 hour period, with detectable levels of expression beginning 6 hrs after a 1:40 seed inoculation with incubation at 30°C, 300 rpm.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can temperatures lower than 30°C be used with MagicMedia even though the suggested incubation temperatures are 37°C and 30°C?

Yes, expression of proteins at 18°C and 25°C has been validated using the Dual Temperature protocol as follows. Day 1: Inoculate LB + antibiotic with an expression colony and incubate overnight at 30°C, 300 rpm. Day 2: Inoculate pre-warmed (30°C) MagicMedia + antibiotic at 1:40 with overnight seed culture. Incubate culture at 30°C, 300 rpm for 6-7 hours or until OD600 reaches >6. Transfer culture to either 18°C or 25°C, 300 rpm and incubate for 24-36 hours. Day 3: Harvest and analyze results.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.