LC3B Antibody Kit for Autophagy - FAQs

How does the LC3B Antibody Kit for Autophagy work?

The LC3B protein plays a critical role in autophagy. This protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3 associates with the phagophore. This localization can be used as a general marker for autophagic membranes. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. The kit also includes chloroquine diphosphate for artificially generating autophagosomes. Following chloroquine diphosphate treatment, lysosomal pH increases and the normal autophagic flux is disrupted, resulting in autophagosome accumulation.

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