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Invitrogen
SSEA3 Monoclonal Antibody (MC-631), DyLight™ 488
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FIGURE: 1 / 3
SSEA3 Antibody (MA1-020-D488) in ICC/IF
Immunofluorescent analysis of SSEA-3 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-3 monoclonal antibody (Product # MA1-020-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834)... View More
Product Details
MA1-020-D488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans
Host/Isotype
Rat
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MC-631
Immunogen
Fischer rat immunized with 4-8 cell stage mouse embryos
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-020-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.
MA1-020-D488 can be used for immunofluoresence analysis of SSEA3 in human iPSCs.
Target Information
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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