Invitrogen

HLA-ABC Monoclonal Antibody (W6/32)

Name: HLA-ABC Monoclonal Antibody (W6\/32)
Brand: Invitrogen
SKU: MA5-11723

5个已发表图片

21 篇参考文献

查看其他62个HLA-ABC抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (8)
已发表图片 (5)

HLA-ABC Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

HLA-ABC Antibody (MA5-11723) in ICC/IF

Immunofluorescent analysis of MHC I HLA-A B C (green) showing staining in the cytoplasm and membrane of non-permeabilized Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an MHC I (HLA-A B C) monoclonal antibody (Product # MA5-11723) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were ... View More

HLA-ABC Antibody in Immunohistochemistry (Frozen) (IHC (F))

HLA-ABC Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image HLA-ABC Monoclonal Antibody (W6/32)

产品信息

MA5-11723

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10-1:200

查看4篇已发表文章 4篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:10-1:200

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流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

W6/32

抗原

Cell membranes of human tonsil lymphocytes

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10985125

产品详细信息

MA5-11723 targets MHC I (HLA-A B C) in IHC (F), ICC/IF, WB and FACS applications and shows reactivity with Human and Mouse samples.

The MA5-11723 immunogen is cell membranes of human tonsil lymphocytes.

靶标信息

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: allele B-3515; B*3509; cell surface glycoprotein; integral membrane protein; exons 2-8; glycosylation aa 86, alpha domain 1 aa 1-24, alpha domain 2 aa 25-114, alpha domain 3 aa 207-298; glycosylation aa 86, alpha domain 1 aa 25-114, alpha domain 2 aa 115-206, alpha domain 3 aa 207-298; H-2K; H-2K(d); H2-K; heavy chain; hla antigen; HLA B-1519; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA protein allele A25; HLA protein allele B37; HLA protein allele B51; HLA protein allele B57; HLA protein allele B7; HLA-A; HLA-A 2402; HLA-A*0201 alpha chain; human leukocyte antigen; HLA-A*0204; HLA-A*0207 alpha chain; human leukocyte antigen; HLA-A-0102 allele; HLA-A24AK; HLA-A25; HLA-B; HLA-B 1502; HLA-B*4002; HLA-B-07ZEL allele; HLA-B-1501; HLA-B-1516; HLA-B-1520 allele; HLA-B-15AOH allele; HLA-B-15ZEL allele; HLA-B-27KSH allele; HLA-B-67LAV allele; This submission reports an HLA-B-6701 variant (HLA-B-67LAV) that differs from the published B-6701 sequence, GenBank Accession Number L17005; HLA-B18; HLA-B27 peptide; HLA-B39; HLA-B41 variant; HLA-B61; HLA-Bw-39-DBU allele; HLA-C; HLA-C antigen; HLA-Cw; HLA-Cw1 protein; HLA-Cw2 protein; HLA-Cw3 protein; human leukocyte antigen; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leucocyte antigen A; leukocyte antigen class I-A; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility antigen HLA-C; major histocompatibility complex class I A; major histocompatibility HLA-Cw2.2 heavy chain; MGC184092; MGC7052; MHC 1; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC class I-like alpha chain membrane-bound type; MHC HLA-A cell surface antigen; MHC HLA-A10-alpha-2 chain precursor; MHC HLA-A2.4a chain; MHC HLA-A24 cell surface glycoprotein; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B44; MHC HLA-B7 heavy chain precursor; MHC HLA-Bw46; MHC HLA-C-alpha-2 chain precursor; MHC I HLA; MHC1; similar to Homo sapiens MHC HLA-B39 mRNA with GenBank Accession Number L42024.1; subtype B*3508 differs at residue 156 (Leu->Arg) with subtype B*3501; unnamed protein product

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基因别名: AS; B-4901; D6S204; HLA-A; HLA-B; HLA-C; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1

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UniProt ID: (Human) P04439, (Human) P01889, (Human) P10321

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Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107

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