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Invitrogen
Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)
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FIGURE: 1 / 20
Phospho-PERK (Thr980) Antibody (MA5-15033) in WB
Western blot was performed using anti-Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4) (Product # MA5-15033) and a 125 kDa band corresponding to Phospho-PERK was induced in RSC96 cells upon thapsigargin and tunicamycin treatments which are known to induce ER stress. Whole cell extracts (30 µg lysate) of RSC96 (Lane1), RSC96 treated with Thapsigargin (300nM for 2hrs) (Lane 2) and RSC96 with Tunicamycin (4... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
G.305.4
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
441 µg/mL
Amount
44.1 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2.7.11.1; eIF-2 alpha kinase; OTTHUMP00000207187; OTTHUMP00000207188; pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; protein tyrosine kinase EIF2AK3
Gene Aliases: PEK
UniProt ID: (Rat) Q9Z1Z1
Entrez Gene ID: (Rat) 29702
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
ATP binding
enzyme binding
protein phosphatase binding
identical protein binding
translation regulator activity
misfolded protein binding
Hsp90 protein binding
protein serine kinase activity
skeletal system development
ossification
angiogenesis
chondrocyte development
translation
regulation of translational initiation
protein phosphorylation
ER overload response
endoplasmic reticulum organization
lactation
positive regulation of signal transduction
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of gene expression
regulation of translational initiation by eIF2 alpha phosphorylation
negative regulation of translation
regulation of fatty acid metabolic process
calcium-mediated signaling
bone mineralization
endoplasmic reticulum unfolded protein response
pancreas development
endocrine pancreas development
negative regulation of myelination
negative regulation of translation in response to stress
SREBP signaling pathway
cellular response to amino acid starvation
response to endoplasmic reticulum stress
regulation of translation initiation in response to endoplasmic reticulum stress
eiF2alpha phosphorylation in response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
ERAD pathway
cellular response to glucose starvation
negative regulation of apoptotic process
regulation of translational initiation in response to stress
fat cell differentiation
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to cold
positive regulation of protein localization to nucleus
negative regulation of translation in response to endoplasmic reticulum stress
regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of glutathione biosynthetic process
response to manganese-induced endoplasmic reticulum stress
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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