NeuroTrace™ BDA-10,000 神经元示踪剂试剂盒
NeuroTrace™ BDA-10,000 神经元示踪剂试剂盒
Invitrogen™

NeuroTrace™ BDA-10,000 神经元示踪剂试剂盒

NeuroTrace™ BDA-10,000 神经元示踪剂试剂盒含有神经元示踪剂、生物素葡聚糖胺 (BDA) 及检测试剂亲和素-辣根过氧化物酶(亲和素-HRP)和二氨基联苯胺 (DAB),用于通过光学或电子显微镜进行后续可视化了解更多信息
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货号数量
N71671 kit
货号 N7167
价格(CNY)
6,895.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
6,895.00
Each
添加至购物车
NeuroTrace™ BDA-10,000 神经元示踪剂试剂盒含有神经元示踪剂、生物素葡聚糖胺 (BDA) 及检测试剂亲和素-辣根过氧化物酶(亲和素-HRP)和二氨基联苯胺 (DAB),用于通过光学或电子显微镜进行后续可视化。
仅供科研使用。不可用于诊断程序。
规格
检测方法Colorimetric
适用于(设备)Electron Microscope, Light Microscope
产品类型神经元示踪剂试剂盒
数量1 kit
运输条件Wet Ice
子细胞定位细胞质&细胞液
产品线NEUROTRACE
Unit SizeEach
内容与储存
在冷冻冰箱(-5 至 -30°C)中避光储存。

常见问题解答 (FAQ)

当我注入荧光葡聚糖时,无法看见神经元的详细结构,如何改善以更好地显示详细的结构?

如果您想看到最精细的结构,应使用低分子量葡聚糖偶联物,例如3,000 MW葡聚糖偶联物。

为什么在固定后,荧光偶联葡聚糖信号没有保留下来?

请确保您使用的是葡聚糖是可固定形式(即包含伯胺)。不包含伯胺的葡聚糖将无法固定。另一个原因可能是葡聚糖的浓度过低,其使用浓度可以增加到10 mg/mL。

右旋糖酐的电荷为多少?

我们没有测定右旋糖酐偶联物的净电荷。净电荷取决于标记右旋糖酐所用的荧光团和制备偶联物所采用的方法。我们根据所用的荧光团将右旋糖酐标记为中性的或阴性的,但是染料和右旋糖酐的净电荷可能并不总是相同。Alexa Fluor、Cascade Blue、荧光黄、荧光素和Oregon Green右旋糖苷本质上是阴性的,而大多数标记两性离子罗丹明 B、四甲基罗丹明和Texas Red染料的右旋糖酐基本上是中性的。

哪种大小的右旋糖酐最适于神经元示踪?

我们已使用过3000至70,000分子量右旋糖酐,但3000至10000分子量右旋糖酐最常用于神经元示踪。3000 分子量的右旋糖酐用于对精细神经传递进行更详细的跟踪、研究间隙连接,并且扩散更快;而10000MW右旋糖酐具有较慢的分散速度、较长的胞内存留时间,且不会不穿过间隙连接。

你们有神经元示踪实验方案吗?

NeuroTrace BDA-10,000 神经元示踪试剂盒(货号N7167)手册有一个 使用10,000 MW lysine-fixable biotin dextran amine(BDA)进行注射操作及神经元跟踪的实验方案。该试验方案也适用于其他荧光标记的右旋糖酐。 请参阅第4和5页的表1a和1b(https://tools.thermofisher.com/content/sfs/manuals/mp07167.pdf)。

引用和文献 (6)

引用和文献
Abstract
Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section.
Authors:Lanciego JL, Wouterlood FG, Erro E, Arribas J, Gonzalo N, Urra X, Cervantes S, Giménez-Amaya JM
Journal:J Neurosci Methods
PubMed ID:11074102
'Experimental neuroanatomical tracing methods lie at the basis of the study of the nervous system. When the scientific question is relatively straightforward, it may be sufficient to derive satisfactory answers from experiments in which a single neuroanatomical tracing method is applied. In various scientific paradigms however, for instance when the ... More
Giant neurons in the macaque pulvinar: a distinct relay subpopulation.
Authors:Imura K, Rockland KS,
Journal:Front Neuroanat
PubMed ID:18958196
Calbindin positive (CB+) giant neurons are known to occur within the pulvinar nucleus in subhuman primates. Here, we demonstrate by combined retrograde tracing and immunocytochemistry that at least some of these are pulvinocortical relay neurons, and further report several distinctive features. First, in contrast with non-giant relay neurons, the giant ... More
Selective anterograde tracing of the individual serotonergic and nonserotonergic components of the dorsal raphe nucleus projection to the vestibular nuclei.
Authors:Halberstadt AL, Balaban CD
Journal:Neuroscience
PubMed ID:17507165
It is well known that the dorsal raphe nucleus (DRN) sends serotonergic and nonserotonergic projections to target regions in the brain stem and forebrain, including the vestibular nuclei. Although retrograde tracing studies have reported consistently that there are differences in the relative innervation of different target regions by serotonergic and ... More
Novel applications of common stereology software to represent the complete distribution, density and spatial organization of anterogradely labelled fibers in neuroanatomical tract-tracing studies.
Authors:Leite-Almeida H, Almeida A
Journal:J Neurosci Methods
PubMed ID:17368789
In a recent study we have adapted for the first time available software used in spatial neuronal organization studies to represent the complete fiber distribution of a brain projection [Leite-Almeida H, Valle-Fernandes A, Almeida A. Brain projections from the medullary dorsal reticular nucleus: an anterograde and retrograde tracing study in ... More
ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.
Authors:
Journal:Cell Death Dis
PubMed ID:31043581