NeuroTrace™ BDA-10,000 Neuronal Tracer Kit - FAQs

如果您想看到最精细的结构，应使用低分子量葡聚糖偶联物，例如3,000 MW葡聚糖偶联物。

请确保您使用的是葡聚糖是可固定形式（即包含伯胺）。不包含伯胺的葡聚糖将无法固定。另一个原因可能是葡聚糖的浓度过低，其使用浓度可以增加到10 mg/mL。

我们没有测定右旋糖酐偶联物的净电荷。净电荷取决于标记右旋糖酐所用的荧光团和制备偶联物所采用的方法。我们根据所用的荧光团将右旋糖酐标记为中性的或阴性的，但是染料和右旋糖酐的净电荷可能并不总是相同。Alexa Fluor、Cascade Blue、荧光黄、荧光素和Oregon Green右旋糖苷本质上是阴性的，而大多数标记两性离子罗丹明 B、四甲基罗丹明和Texas Red染料的右旋糖酐基本上是中性的。

我们已使用过3000至70,000分子量右旋糖酐，但3000至10000分子量右旋糖酐最常用于神经元示踪。3000 分子量的右旋糖酐用于对精细神经传递进行更详细的跟踪、研究间隙连接，并且扩散更快；而10000MW右旋糖酐具有较慢的分散速度、较长的胞内存留时间，且不会不穿过间隙连接。

NeuroTrace BDA-10,000 神经元示踪试剂盒（货号N7167）手册有一个 使用10,000 MW lysine-fixable biotin dextran amine（BDA）进行注射操作及神经元跟踪的实验方案。该试验方案也适用于其他荧光标记的右旋糖酐。 请参阅第4和5页的表1a和1b（https://tools.thermofisher.com/content/sfs/manuals/mp07167.pdf）。

详细信息请查阅此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html）。

If you want to see the most detailed structure you should use the low molecular weight conjugated dextrans such as the 3,000 MW dextrans.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Ensure that the dextran you are using is the fixable form (i.e., contains a primary amine). Dextrans that do not contain a primary amine will not be fixed. Another factor could be that the concentration of the dextran is too low, and the concentration use can be increased up to 10 mg/mL.

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We do not determine the net charge of the dextran conjugates. The net charge depends on the fluorophore used to label the dextran and the method of preparing the conjugate. We label some dextrans as neutral or anionic based on the fluorophore used, however the net charge of the dextran may not always be the same as the dye. The Alexa Fluor, Cascade Blue, Lucifer Yellow, fluorescein, and Oregon Green dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic Rhodamine B, tetramethylrhodamine and Texas Red dyes are essentially neutral.

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Dextrans with molecular weights from 3,000 to 70,000 have been used, however the 3,000 and 10,000 MW dextrans are most commonly used for neuronal tracing. The 3,000 MW dextrans are used for more detailed tracing of fine neuronal projections, investigating gap junctions, and diffuse more quickly; while the 10,000 MW dextrans have slower distribution, longer cellular retention, and do not cross gap junctions.

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The NeuroTrace BDA-10,000 Neuronal Tracer Kit (Cat. No. N7167) manual has a good protocol for injection procedures and neuronal tracing using the10,000 MW lysine-fixable biotin dextran amine (BDA). This protocol could potentially be applied to other fluorescent dextrans.

Please review Tables 1a and 1b on pages 4 and 5 - https://tools.thermofisher.com/content/sfs/manuals/mp07167.pdf

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.