'The human ov-serpin monocyte neutrophil elastase inhibitor (MNEI) is encoded by a single gene SERPINB1. It is a highly efficient inhibitor of neutrophil granule proteases. Four murine genes with high sequence identity with MNEI were identified and fully sequenced, and these were named EIA, EIB, EIC, and EID. EIA, EIB ... More
Novel Modulation of Neuronal Nicotinic Acetylcholine Receptors by Association with the Endogenous Prototoxin lynx1.
We previously identified lynx1 as a neuronal membrane molecule related to snake alpha-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) ... More
Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease.
AuthorsZheng L, Nukuna B, Brennan ML, Sun M, Goormastic M, Settle M, Schmitt D, Fu X, Thomson L, Fox PL, Ischiropoulos H, Smith JD, Kinter M, Hazen SL,
JournalJ Clin Invest
PubMed ID15314690
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO(2)Tyr) and myeloperoxidase (MPO), a protein that catalyzes generation of nitrating oxidants, serve as independent predictors of atherosclerotic risk, burden, and incident cardiac events. We now show both that apolipoprotein A-I (apoA-I), the primary protein constituent of HDL, is ... More
In vivo functional assay of a recombinant aquaporin in Pichia pastoris.
AuthorsDaniels MJ, Wood MR, Yeager M,
JournalAppl Environ Microbiol
PubMed ID16461705
The water channel protein PvTIP3;1 (alpha-TIP) is a member of the major intrinsic protein (MIP) membrane channel family. We overexpressed this eukaryotic aquaporin in the methylotrophic yeast Pichia pastoris, and immunogold labeling of cellular cryosections showed that the protein accumulated in the plasma membrane, as well as vacuolar and other ... More
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase.
AuthorsChattopadhyay MK, Tabor CW, Tabor H,
JournalProc Natl Acad Sci U S A
PubMed ID14617780
In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for the ... More
The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.
Authors Hill Jennifer J; Davies Monique V; Pearson Adele A; Wang Jack H; Hewick Rodney M; Wolfman Neil M; Qiu Yongchang;
JournalJ Biol Chem
PubMed ID12194980
Myostatin, also known as growth and differentiation factor 8, is a member of the transforming growth factor beta superfamily that negatively regulates skeletal muscle mass (1). Recent experiments have shown that myostatin activity is detected in serum by a reporter gene assay only after activation by acid, suggesting that native ... More
4E-binding proteins, the suppressors of eukaryotic initiation factor 4E, are down-regulated in cells with acquired or intrinsic resistance to rapamycin.
Authors Dilling Michael B; Germain Glen S; Dudkin Lorina; Jayaraman Arun L; Zhang Xiongwen; Harwood Franklin C; Houghton Peter J;
JournalJ Biol Chem
PubMed ID11847216
To determine whether inhibition of either the ribosomal p70 S6 kinase or eukaryotic initiation factor (eIF) 4E pathways downstream of the mammalian target of rapamycin, mTOR, contributes to rapamycin-induced growth arrest, clones of Rh30 rhabdomyosarcoma cells were selected for rapamycin resistance. Expression of c-Myc and anchorage-independent growth were enhanced in ... More
Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability.
Authors Dahlgren Karie N; Manelli Arlene M; Stine W Blaine Jr; Baker Lorinda K; Krafft Grant A; LaDu Mary Jo;
JournalJ Biol Chem
PubMed ID12058030
Genetic evidence predicts a causative role for amyloid-beta (A beta) in Alzheimer's disease. Recent debate has focused on whether fibrils (amyloid) or soluble oligomers of A beta are the active species that contribute to neurodegeneration and dementia. We developed two aggregation protocols for the consistent production of stable oligomeric or ... More
Association of human aging with a functional variant of klotho.
Authors Arking Dan E; Krebsova Alice; Macek Milan Sr; Macek Milan Jr; Arking Albert; Mian I Saira; Fried Linda; Hamosh Ada; Dey Srabani; McIntosh Iain; Dietz Harry C;
JournalProc Natl Acad Sci U S A
PubMed ID11792841
Mice deficient in Klotho gene expression exhibit a syndrome resembling premature human aging. To determine whether variation in the human KLOTHO locus contributes to survival, we applied two newly characterized polymorphic microsatellite markers flanking the gene in a population-based association study. In a cohort chosen for its homogeneity, Bohemian Czechs, ... More