Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
Authors:Blondal T, Hjorleifsdottir SH, Fridjonsson OF, Aevarsson A, Skirnisdottir S, Hermannsdottir AG, Hreggvidsson GO, Smith AV, Kristjansson JK
Journal:Nucleic Acids Res
PubMed ID:14654700
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of ... More
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease.
Authors:Ott SJ, Musfeldt M, Wenderoth DF, Hampe J, Brant O, Fölsch UR, Timmis KN, Schreiber S
Journal:Gut
PubMed ID:15082587
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated ... More
Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection.
Authors:Knott AW, Juno RJ, Jarboe MD, Profitt SA, Erwin CR, Smith EP, Fagin JA, Warner BW
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:15142831
'Prior studies of intestinal adaptation after massive small bowel resection (SBR) have focused on growth factors and their effects on amplification of the gut mucosa. Because adaptive changes have also been described in intestinal smooth muscle, we sought to determine the effect of targeted smooth muscle growth factor overexpression on ... More
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
Authors:Kemp MG, Ghosh M, Liu G, Leffak M,
Journal:Nucleic Acids Res
PubMed ID:15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent ... More
Fast separation of single-stranded oligonucleotides by capillary electrophoresis using OliGreen as fluorescence inducing agent.
Authors:Zhang J, Liang D, He W, Wan F, Ying Q, Chu B
Journal:Electrophoresis
PubMed ID:16315172
'The fast separation of oligonucleotide (oligos) sizing marker by CE using OliGreen and including effects due to the concentration of separation medium and urea denaturant is presented. OliGreen dye is found to be more sensitive than ethidium bromide (by a factor of about 6 based on S/N considerations) for the ... More