Quant-iT™ OliGreen™ ssDNA 定量试剂盒和 Quant-iT OliGreen ssDNA 试剂
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
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Quant-iT™ OliGreen™ ssDNA 定量试剂盒和 Quant-iT OliGreen ssDNA 试剂

Quant-iT™ OliGreen ssDNA 定量试剂盒和 Quant-iT OliGreen ssDNA 试剂含有对溶液中的寡核苷酸和单链 DNA (ssDNA) 进行定量的超灵敏绿色荧光核酸染色剂。
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货号数量产品类型
O75821 mLQuant-iT OliGreen ssDNA 试剂
O114921 kitQuant-iT OliGreen ssDNA 试剂盒
货号 O7582
价格(CNY)
5,769.00
飞享价
Ends: 31-Dec-2025
7,428.00
共减 1,659.00 (22%)
Each
添加至购物车
数量:
1 mL
产品类型:
Quant-iT OliGreen ssDNA 试剂
价格(CNY)
5,769.00
飞享价
Ends: 31-Dec-2025
7,428.00
共减 1,659.00 (22%)
Each
添加至购物车
使用 Quant-iT OliGreen ssDNA 定量试剂盒和试剂轻松定量 ssDNA。该定量试剂盒和试剂无需高浓度样品即可对单链 DNA 进行灵敏定量。使用该定量试剂盒或 Quant-iT OliGreen ssDNA 试剂可定量低至 100 pg/mL 的样品。
使用 Quant-iT OliGreen ssDNA 测定试剂盒和 Quant-iT OliGreen ssDNA 试剂准确定量 ssDNA 和其他寡核苷酸,无需高浓度样品。Quant-iT OliGreen ssDNA 测定试剂盒含有对溶液中的寡核苷酸和单链 DNA (ssDNA) 进行定量的超灵敏绿色荧光核酸染色剂。Quant-iT™ OliGreen™ ssDNA 测定试剂,让研究人员可以利用荧光酶标仪的激发和发射波长,对低至 1 ng/mL 的寡核苷酸或 ssDNA(200 µL 测定体积中含 200 pg)进行定量。该灵敏度超过吸光度方法的10,000倍。

除染料外,OliGreen ssDNA 测定试剂盒还包括缓冲液和寡核苷酸标准品。OliGreen 试剂也可作为独立的 Quant-iT OliGreen ssDNA 试剂提供。

仅供科研使用。不可用于诊断程序。
规格
激发/发射500/525
适用于(设备)微孔板读数仪
反应次数200次测定(2 mL 测定体积)
产品线OLIGREEN、Quant-iT
产品类型Quant-iT OliGreen ssDNA 试剂
定量范围200 pg 至 2 μg
数量1 mL
运输条件室温
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

为什么我用Qubit Assay检测时会得到负的荧光值?

负的荧光值物理上是不可能的。它是由于软件自动扣除背景信号而造成的假象。这意味着你的荧光计检测到背景信号并将其扣除从而牺牲了真实数据。务必做一个仅有缓冲液的对照并评估信号的类型。你可能需要换用另外一块板。

我有一个Quant-iT DNA试剂盒,而想在Qubit荧光计上使用。可以吗?

是的,使用手册中有关于此项应用的说明。你将使用0 ng/μL lambda dsDNA HS标准品制备Standard #1。你将稀释一个10 ng/μL lambda dsDNA HS标准品得到Standard #2。然后你准备样本并将它们和上述的两点标准曲线进行比较。Quant-iT dsDNA BR试剂盒也可以用类似的方式使用。

这些Quant-iT试剂盒的适用pH范围是多少?

试剂盒中的缓冲液将保证合适的pH值范围,即使您的DNA处于这一pH范围之外也没关系,因为检测中所用的缓冲液体积至少超过样本体积的10倍。

我试图对一些带有荧光基团标记的DNA进行定量。这可行吗?

PicoGreen染料和其它基于荧光定量的试剂不建议用于对染料偶联的核酸进行定量。核酸上携带的染料基团会干扰定量试剂的结合或荧光产生。

DNA长度对dsDNA检测结果有影响吗?

大致在20-mer或更短范围内的链信号水平较低。对于大部分由短链组成的dsDNA样本,仍可使用试剂,但应使用与样本长度相当的dsDNA标准品。

引用和文献 (52)

引用和文献
Abstract
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
Authors:Blondal T, Hjorleifsdottir SH, Fridjonsson OF, Aevarsson A, Skirnisdottir S, Hermannsdottir AG, Hreggvidsson GO, Smith AV, Kristjansson JK
Journal:Nucleic Acids Res
PubMed ID:14654700
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of ... More
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease.
Authors:Ott SJ, Musfeldt M, Wenderoth DF, Hampe J, Brant O, Fölsch UR, Timmis KN, Schreiber S
Journal:Gut
PubMed ID:15082587
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated ... More
Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection.
Authors:Knott AW, Juno RJ, Jarboe MD, Profitt SA, Erwin CR, Smith EP, Fagin JA, Warner BW
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:15142831
'Prior studies of intestinal adaptation after massive small bowel resection (SBR) have focused on growth factors and their effects on amplification of the gut mucosa. Because adaptive changes have also been described in intestinal smooth muscle, we sought to determine the effect of targeted smooth muscle growth factor overexpression on ... More
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
Authors:Kemp MG, Ghosh M, Liu G, Leffak M,
Journal:Nucleic Acids Res
PubMed ID:15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent ... More
Fast separation of single-stranded oligonucleotides by capillary electrophoresis using OliGreen as fluorescence inducing agent.
Authors:Zhang J, Liang D, He W, Wan F, Ying Q, Chu B
Journal:Electrophoresis
PubMed ID:16315172
'The fast separation of oligonucleotide (oligos) sizing marker by CE using OliGreen and including effects due to the concentration of separation medium and urea denaturant is presented. OliGreen dye is found to be more sensitive than ethidium bromide (by a factor of about 6 based on S/N considerations) for the ... More