Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
AuthorsBlondal T, Hjorleifsdottir SH, Fridjonsson OF, Aevarsson A, Skirnisdottir S, Hermannsdottir AG, Hreggvidsson GO, Smith AV, Kristjansson JK
JournalNucleic Acids Res
PubMed ID14654700
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of ... More
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease.
AuthorsOtt SJ, Musfeldt M, Wenderoth DF, Hampe J, Brant O, Fölsch UR, Timmis KN, Schreiber S
JournalGut
PubMed ID15082587
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated ... More
Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection.
AuthorsKnott AW, Juno RJ, Jarboe MD, Profitt SA, Erwin CR, Smith EP, Fagin JA, Warner BW
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15142831
'Prior studies of intestinal adaptation after massive small bowel resection (SBR) have focused on growth factors and their effects on amplification of the gut mucosa. Because adaptive changes have also been described in intestinal smooth muscle, we sought to determine the effect of targeted smooth muscle growth factor overexpression on ... More
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
AuthorsKemp MG, Ghosh M, Liu G, Leffak M,
JournalNucleic Acids Res
PubMed ID15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent ... More
Fast separation of single-stranded oligonucleotides by capillary electrophoresis using OliGreen as fluorescence inducing agent.
AuthorsZhang J, Liang D, He W, Wan F, Ying Q, Chu B
JournalElectrophoresis
PubMed ID16315172
'The fast separation of oligonucleotide (oligos) sizing marker by CE using OliGreen and including effects due to the concentration of separation medium and urea denaturant is presented. OliGreen dye is found to be more sensitive than ethidium bromide (by a factor of about 6 based on S/N considerations) for the ... More
The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation.
AuthorsLi MG, Serr M, Newman EA, Hays TS
JournalMol Biol Cell
PubMed ID15090621
'Variations in subunit composition and modification have been proposed to regulate the multiple functions of cytoplasmic dynein. Here, we examine the role of the Drosophila ortholog of tctex-1, the 14-kDa dynein light chain. We show that the 14-kDa light chain is a bona fide component of Drosophila cytoplasmic dynein and ... More
Rapid enumeration of respiratory viruses.
AuthorsFerris MM, McCabe MO, Doan LG, Rowlen KL
JournalAnal Chem
PubMed ID11985317
'Virus detection and enumeration has become increasingly important in fields ranging from medicine and biotechnology to environmental science. Although there are a wide variety of techniques that can be used to count viruses, there is demand for a rapid and more accurate means for virus enumeration. In this work, the ... More
Interplay of polyethyleneimine molecular weight and oligonucleotide backbone chemistry in the dynamics of antisense activity.
AuthorsSundaram S, Lee LK, Roth CM
JournalNucleic Acids Res
PubMed ID17576672
'The widespread utilization of gene silencing techniques, such as antisense, is impeded by the poor cellular delivery of oligonucleotides (ONs). Rational design of carriers for enhanced ON delivery demands a better understanding of the role of the vector on the extent and time course of antisense effects. The aim of ... More
Quantitative determination of short single-stranded oligonucleotides from blood plasma using capillary electrophoresis with laser-induced fluorescence.
AuthorsReyderman L, Stavchansky S
JournalAnal Chem
PubMed ID9271066
'The quantitative determination of short (< 20 bases) single-standed (ss-) oligonucleotides (oligos) from blood plasma using capillary gel electrophoresis with laser-induced fluorescence is reported. Oligos were derivatized on column after equilibration of the column with a 1:150 dilution of OliGreen dye. The resulting fluorescent complex was detected and measured with ... More
Incorporation of CpG oligonucleotide ligand into protein-loaded particle vaccines promotes antigen-specific CD8 T-cell immunity.
AuthorsStandley SM, Mende I, Goh SL, Kwon YJ, Beaudette TT, Engleman EG, Fréchet JM
JournalBioconjug Chem
PubMed ID17226959
'The development of multicomponent biotherapeutic carriers is an important challenge in the field of drug delivery, particularly in the area of protein-based vaccines. While the delivery of protein antigens to antigen presenting cells (APCs) is crucial for this type of vaccination, the incorporation of additional adjuvants may be just as ... More
Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry.
AuthorsZimmerman WC, Sillibourne J, Rosa J, Doxsey SJ
JournalMol Biol Cell
PubMed ID15146056
'Microtubule nucleation is the best known function of centrosomes. Centrosomal microtubule nucleation is mediated primarily by gamma tubulin ring complexes (gamma TuRCs). However, little is known about the molecules that anchor these complexes to centrosomes. In this study, we show that the centrosomal coiled-coil protein pericentrin anchors gamma TuRCs at ... More
Two-way arginine transport in human endothelial cells: TNF-alpha stimulation is restricted to system y(+).
AuthorsSala R, Rotoli BM, Colla E, Visigalli R, Parolari A, Bussolati O, Gazzola GC, Dall'Asta V
JournalAm J Physiol Cell Physiol
PubMed ID11742806
'Human umbilical vein endothelial cells transport arginine through two Na(+)-independent systems. System y(+)L is insensitive to N-ethylmaleimide (NEM), inhibited by L-leucine in the presence of Na(+), and referable to the expression of SLC7A6/y(+)LAT2, SLC7A7/y(+)LAT1, and SLC3A2/4F2hc. System y(+) is referable to the expression of SLC7A1/CAT1 and SLC7A2/CAT2B. Tumor necrosis factor-alpha ... More
A mutant Drosophila insulin receptor homolog that extends life-span and impairs neuroendocrine function.
AuthorsTatar M, Kopelman A, Epstein D, Tu MP, Yin CM, Garofalo RS
JournalScience
PubMed ID11292875
'The Drosophila melanogaster gene insulin-like receptor (InR) is homologous to mammalian insulin receptors as well as to Caenorhabditis elegans daf-2, a signal transducer regulating worm dauer formation and adult longevity. We describe a heteroallelic, hypomorphic genotype of mutant InR, which yields dwarf females with up to an 85% extension of ... More
Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci.
AuthorsTulin A, Spradling A
JournalScience
PubMed ID12543974
'Steroid response and stress-activated genes such as hsp70 undergo puffing in Drosophila larval salivary glands, a local loosening of polytene chromatin structure associated with gene induction. We find that puffs acquire elevated levels of adenosine diphosphate (ADP)-ribose modified proteins and that poly(ADP)-ribose polymerase (PARP) is required to produce normal-sized puffs ... More
Determination of single-stranded oligodeoxynucleotides by capillary gel electrophoresis with laser induced fluorescence and on column derivatization.
AuthorsReyderman L, Stavchansky S
JournalJ Chromatogr A
PubMed ID8997752
OliGreen reagent, a new dye for complexing of oligonucleotides of low molecular mass was successfully utilized for their quantitative analysis using on column derivatization and capillary gel electrophoresis with laser induced fluorescence. Method optimization for the precise, accurate and reproducible quantitation of low-molecular-mass oligonucleotides from aqueous matrices is described. ... More
Automated capture and on-column detection of biotinylated DNA on a disposable solid support.
AuthorsDecuir M, Lähdesmäki I, Carroll AD, Ruzicka J
JournalAnalyst
PubMed ID17646882
This work comprises the development of a technique for the capture of single-stranded DNA on a solid support combined with in situ quantification. The capture is based on the strong and selective interaction between biotinylated DNA and streptavidin-coated agarose beads. Sequential Injection in the lab-on-valve format allows for automated manipulation ... More
Gene expression analysis by transcript profiling coupled to a gene database query.
AuthorsShimkets RA, Lowe DG, Tai JT, Sehl P, Jin H, Yang R, Predki PF, Rothberg BE, Murtha MT, Roth ME, Shenoy SG, Windemuth A, Simpson JW, Simons JF, Daley MP, Gold SA, McKenna MP, Hillan K, Went GT, Rothberg JM
JournalNat Biotechnol
PubMed ID10429247
We describe an mRNA profiling technique for determining differential gene expression that utilizes, but does not require, prior knowledge of gene sequences. This method permits high-throughput reproducible detection of most expressed sequences with a sensitivity of greater than 1 part in 100,000. Gene identification by database query of a restriction ... More
High-density fiber-optic genosensor microsphere array capable of zeptomole detection limits.
Authors Epstein Jason R; Lee Myoyong; Walt David R;
JournalAnal Chem
PubMed ID11985315
The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-microm-diameter microspheres on the distal face of a 500-microm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the ... More
Role of delayed nuclear envelope breakdown and mitosis in Wolbachia-induced cytoplasmic incompatibility.
AuthorsTram U, Sullivan W
JournalScience
PubMed ID12004132
The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI resulted from delayed nuclear envelope breakdown of the male pronucleus in Nasonia vitripennis. This caused asynchrony between the male and female pronuclei and, ultimately, loss of paternal chromosomes ... More
FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation.
AuthorsDubrulle J, McGrew MJ, Pourquié O
JournalCell
PubMed ID11511349
Vertebrate segmentation requires a molecular oscillator, the segmentation clock, acting in presomitic mesoderm (PSM) cells to set the pace at which segmental boundaries are laid down. However, the signals that position each boundary remain unclear. Here, we report that FGF8 which is expressed in the posterior PSM, generates a moving ... More
Drosophila checkpoint kinase 2 couples centrosome function and spindle assembly to genomic integrity.
AuthorsTakada S, Kelkar A, Theurkauf WE
JournalCell
PubMed ID12679037
In syncytial Drosophila embryos, damaged or incompletely replicated DNA triggers centrosome disruption in mitosis, leading to defects in spindle assembly and anaphase chromosome segregation. The damaged nuclei drop from the cortex and are not incorporated into the cells that form the embryo proper. A null mutation in the Drosophila checkpoint ... More
The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly.
AuthorsGandhi R, Bonaccorsi S, Wentworth D, Doxsey S, Gatti M, Pereira A
JournalMol Biol Cell
PubMed ID13679514
We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis. Mutant cells ... More
I5S: wide-field light microscopy with 100-nm-scale resolution in three dimensions.
AuthorsShao L, Isaac B, Uzawa S, Agard DA, Sedat JW, Gustafsson MG,
JournalBiophys J
PubMed ID18326649
A new type of wide-field fluorescence microscopy is described, which produces 100-nm-scale spatial resolution in all three dimensions, by using structured illumination in a microscope that has two opposing objective lenses. Illumination light is split by a grating and a beam splitter into six mutually coherent beams, three of which ... More
Mutations in the alpha-tubulin 67C gene specifically impair achiasmate segregation in Drosophila melanogaster.
Drosophila melanogaster oocytes heterozygous for mutations in the alpha-tubulin 67C gene (alphatub67C) display defects in centromere positioning during prometaphase of meiosis I. The centromeres do not migrate to the poleward edges of the chromatin mass, and the chromatin fails to stretch during spindle lengthening. These results suggest that the poleward ... More
l(3)malignant brain tumor and three novel genes are required for Drosophila germ-cell formation.
AuthorsYohn CB, Pusateri L, Barbosa V, Lehmann R
JournalGenetics
PubMed ID14704174
To identify genes involved in the process of germ-cell formation in Drosophila, a maternal-effect screen using the FLP/FRT-ovoD method was performed on chromosome 3R. In addition to expected mutations in the germ-cell determinant oskar and in other genes known to be involved in the process, several novel mutations caused defects ... More
Flow cytometric detection of viruses.
AuthorsBrussaard CP, Marie D, Bratbak G
JournalJ Virol Methods
PubMed ID10716350
Representatives from several different virus families (Baculoviridae, Herpesviridae, Myoviridae, Phycodnaviridae, Picornaviridae, Podoviridae, Retroviridae, and Siphoviridae) were stained using a variety of highly fluorescent nucleic acid specific dyes (SYBR Green I, SYBR Green II, OliGreen, PicoGreen) and examined using a standard flow cytometer equipped with a standard 15 mW argon-ion laser. ... More
An essential role of DmRad51/SpnA in DNA repair and meiotic checkpoint control.
AuthorsStaeva-Vieira E, Yoo S, Lehmann R
JournalEMBO J
PubMed ID14592983
Rad51 is a conserved protein essential for recombinational repair of double-stranded DNA breaks (DSBs) in somatic cells and during meiosis in germ cells. Yeast Rad51 mutants are viable but show meiosis defects. In the mouse, RAD51 deletions cause early embryonic death, suggesting that in higher eukaryotes Rad51 is required for ... More
Transcriptional activation of the human brain-derived neurotrophic factor gene promoter III by dopamine signaling in NT2/N neurons.
AuthorsFang H, Chartier J, Sodja C, Desbois A, Ribecco-Lutkiewicz M, Walker PR, Sikorska M
JournalJ Biol Chem
PubMed ID12738784
We have identified a functional cAMP-response element (CRE) in the human brain-derived neurotrophic factor (BDNF) gene promoter III and established that it participated in the modulation of BDNF expression in NT2/N neurons via downstream signaling from the D1 class of dopamine (DA) receptors. The up-regulation of BDNF expression, in turn, ... More
Nucleic acid detection using non-radioactive labelling methods.
AuthorsMansfield ES, Worley JM, McKenzie SE, Surrey S, Rappaport E, Fortina P
JournalMol Cell Probes
PubMed ID7477006
Nucleic acid probe-based assays are now widely used in genetic research, human identification, forensics and in a broad spectrum of clinical assays in the fields of microbiology, haematology/oncology and virology. Labelled probes are used in a variety of assay formats including dot-blots, Southern blots (DNA target), Northern blots (RNA target), ... More
Mechanisms underlying DNA damage resistance in a Xiphophorus melanoma cell line.
AuthorsMoredock S, Nairn RS, Johnston DA, Byrom M, Heaton G, Lowery M, Mitchell DL
JournalCarcinogenesis
PubMed ID12949045
The Xiphophorus hybrid fish model is an important resource for investigating the genetics and molecular biology of melanoma. Consistent with studies using human melanoma cell lines, the Xiphophorus melanoma cell line PSM, survives the lethal effects of ultraviolet-B radiation (UV-B) radiation much better than a cell line derived from normal ... More
Detection of genomic polymorphisms associated with venous thrombosis using the invader biplex assay.
AuthorsPatnaik M, Dlott JS, Fontaine RN, Subbiah MT, Hessner MJ, Joyner KA, Ledford MR, Lau EC, Moehlenkamp C, Amos J, Zhang B, Williams TM
JournalJ Mol Diagn
PubMed ID15096570
A multi-site study to assess the accuracy and performance of the biplex Invader assay for genotyping five polymorphisms implicated in venous thrombosis was carried out in seven laboratories. Genotyping results obtained using the Invader biplex assay were compared to those obtained from a reference method, either allele-specific polymerase chain reaction ... More
Validation of a high-throughput in vitro alkaline elution/rat hepatocyte assay for DNA damage.
In vitro alkaline elution is a sensitive and specific short term assay which measures DNA strand breakage in a mammalian test system (primary rat hepatocytes). This lab has previously demonstrated the performance of the assay with known genotoxic and non-genotoxic compounds. The methodology employed has relatively low sample throughput and ... More
Drosophila Hfp negatively regulates dmyc and stg to inhibit cell proliferation.
AuthorsQuinn LM, Dickins RA, Coombe M, Hime GR, Bowtell DD, Richardson H
JournalDevelopment
PubMed ID14993190
Mammalian FIR has dual roles in pre-mRNA splicing and in negative transcriptional control of Myc. Here we show that Half pint (Hfp), the Drosophila orthologue of FIR, inhibits cell proliferation in Drosophila. We find that Hfp overexpression potently inhibits G1/S progression, while hfp mutants display ectopic cell cycles. Hfp negatively ... More
SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila.
AuthorsZallen JA, Cohen Y, Hudson AM, Cooley L, Wieschaus E, Schejter ED
JournalJ Cell Biol
PubMed ID11854309
The Arp2/3 complex and its activators, Scar/WAVE and Wiskott-Aldrich Syndrome protein (WASp), promote actin polymerization in vitro and have been proposed to influence cell shape and motility in vivo. We demonstrate that the Drosophila Scar homologue, SCAR, localizes to actin-rich structures and is required for normal cell morphology in multiple ... More
Multiple functional elements comprise a Mammalian chromosomal replicator.
AuthorsLiu G, Malott M, Leffak M
JournalMol Cell Biol
PubMed ID12589000
The structure of replication origins in metazoans is only nominally similar to that in model organisms, such as Saccharomyces cerevisiae. By contrast to the compact origins of budding yeast, in metazoans multiple elements act as replication start sites or control replication efficiency. We first reported that replication forks diverge from ... More
A whole new way of looking at things: the use of Dark Reader technology to detect fluorophors.
AuthorsSeville M
JournalElectrophoresis
PubMed ID11332748
The Dark Reader optical system (Clare Chemical Research, Denver, CO, USA) uses relatively low intensity broad-band visible blue light in combination with broad-band optical filters to detect fluorescence with a level of sensitivity that often surpasses that of UV transilluminators and can rival that of laser-based scanners. Applications of DR ... More
Sequencing-independent method to generate oligonucleotide probes targeting a variable region in bacterial 16S rRNA by PCR with detachable primers.
AuthorsBertilsson S, Cavanaugh CM, Polz MF
JournalAppl Environ Microbiol
PubMed ID12450831
Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bacteria in natural environments. We developed a PCR-based approach that allows synthesis of oligonucleotide probes targeting a variable region in the 16S rRNA without prior knowledge of the target sequence. Analysis of all 16S rRNA gene sequences ... More
High-sensitivity array analysis of gene expression for the early detection of disseminated breast tumor cells in peripheral blood.
AuthorsMartin KJ, Graner E, Li Y, Price LM, Kritzman BM, Fournier MV, Rhei E, Pardee AB
JournalProc Natl Acad Sci U S A
PubMed ID11226293
Early detection is an effective means of reducing cancer mortality. Here, we describe a highly sensitive high-throughput screen that can identify panels of markers for the early detection of solid tumor cells disseminated in peripheral blood. The method is a two-step combination of differential display and high-sensitivity cDNA arrays. In ... More
Rapid measurement of modified oligonucleotide levels in plasma samples with a fluorophore specific for single-stranded DNA.
AuthorsGray GD, Wickstrom E
JournalAntisense Nucleic Acid Drug Dev
PubMed ID9212903
Animal studies of therapeutic oligonucleotides require measurement of circulating levels of oligonucleotides by multistep, time-consuming methods. In contrast, addition of a single-stranded DNA binding fluorophore, OliGreen, to oligonucleotides in plasma samples allowed rapid quantitation. Dose-response curves were measured for five different oligonucleotide analogs added to plasma or serum. Phosphorothioate or ... More
Spontaneous entrapment of polynucleotides upon electrostatic interaction with ethanol-destabilized cationic liposomes.
AuthorsMaurer N, Wong KF, Stark H, Louie L, McIntosh D, Wong T, Scherrer P, Semple SC, Cullis PR
JournalBiophys J
PubMed ID11325732
This study describes the effect of ethanol and the presence of poly(ethylene) glycol (PEG) lipids on the interaction of nucleotide-based polyelectrolytes with cationic liposomes. It is shown that preformed large unilamellar vesicles (LUVs) containing a cationic lipid and a PEG coating can be induced to entrap polynucleotides such as antisense ... More
Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples.
AuthorsBak ST, Staunstrup NH, Starnawska A, Daugaard TF, Nyengaard JR, Nyegaard M, Børglum A, Mors O, Dorph-Petersen KA, Nielsen AL
JournalMol Neurobiol
PubMed ID27995571
'We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the ... More
Phosphatidylserine decarboxylase is critical for the maintenance of skeletal muscle mitochondrial integrity and muscle mass.
AuthorsSelathurai A, Kowalski GM, Mason SA, Callahan DL, Foletta VC, Della Gatta PA, Lindsay A, Hamley S, Kaur G, Curtis AR, Burch ML, Ang T, McGee SL, Bruce CR
JournalMol Metab
PubMed ID31285171
'Phosphatidylethanolamine (PtdEtn) is a major phospholipid in mammals. It is synthesized via two pathways, the CDP-ethanolamine pathway in the endoplasmic reticulum and the phosphatidylserine (PtdSer) decarboxylase (PSD) pathway in the mitochondria. While the CDP-ethanolamine pathway is considered the major route for PtdEtn synthesis in most mammalian tissues, little is known ... More
Aptamer-functionalized albumin-based nanoparticles for targeted drug delivery.
AuthorsXu L, He XY, Liu BY, Xu C, Ai SL, Zhuo RX, Cheng SX
JournalColloids Surf B Biointerfaces
PubMed ID30005287
'Proteins have been extensively explored as versatile nanocarriers for drug delivery due to their complete biocompatibility, ease of surface modification, and lack of toxicity and immunogenicity. In this study, a facile strategy was used to construct aptamer-functionalized albumin-based nanoparticles for effective drug delivery and targeted cancer therapy. A hydrophobic drug, ... More
Reversal of tumor malignization and modulation of cell behaviors through genome editing mediated by a multi-functional nanovector.
AuthorsLiu BY, He XY, Zhuo RX, Cheng SX
JournalNanoscale
PubMed ID30417194
'To effectively reverse tumor malignization by genome editing, a multi-functional self-assembled nanovector for the delivery of a genome editing plasmid specifically to tumor cells was developed. The nanovector core consisting of protamine and calcium carbonate entrapping the CRISPR-Cas9 plasmid is decorated by aptamer incorporated heparin. Owing to a high affinity ... More
Structurally unique PARP-1 inhibitors for the treatment of prostate cancer.
AuthorsDivan A, Sibi MP, Tulin A
JournalPharmacol Res Perspect
PubMed ID32342655
'The prognosis for metastatic castration-resistant prostate cancer is unfavorable, and although Poly(ADP)-ribose polymerase-1 (PARP-1) inhibitors have shown efficacy in the treatment of androgen-receptor dependent malignancies, the limited number of options present obstacles for patients that are not responsive to these treatments. Here we utilize an integrated screening strategy that combines ... More
Layer-by-layer assembled gold nanoshells for the intracellular delivery of miR-34a.
AuthorsGoyal R, Kapadia CH, Melamed JR, Riley RS, Day ES
JournalCell Mol Bioeng
PubMed ID30555597
'MicroRNAs (miRNAs) are short noncoding RNAs whose ability to regulate the expression of multiple genes makes them potentially exciting tools to treat disease. Unfortunately, miRNAs cannot passively enter cells due to their hydrophilicity and negative charge. Here, we report the development of layer-by-layer assembled nanoshells (LbL-NS) as vehicles for efficient ... More
Dysregulation of microRNA biogenesis machinery and microRNA/RNA ratio in skeletal muscle of amyotrophic lateral sclerosis mice.
AuthorsRussell AP, Ghobrial L, Ngo S, Yerbury J, Zacharewicz E, Chung R, Lamon S
JournalMuscle Nerve
PubMed ID29236291
The pathology of amyotrophic lateral sclerosis (ALS) is associated with impaired RNA processing and microRNA (miRNA) dysregulation. Here we investigate the regulation of the members of the miRNA biogenesis pathways and total miRNA levels at different stages of the disease. ... More
Trypsin-Amplified Aerolysin Nanopore Amplified Sandwich Assay for Attomolar Nucleic Acid and Single Bacteria Detection.
AuthorsSong F, Deng R, Liu H, Wang A, Ma C, Wei Y, Cui X, Wan Y, Li J
JournalAnal Chem
PubMed ID31577421
Nanopore technology is promising for the next-generation of nucleic acid-based diagnosis. However, sequence reservation could still be hardly achieved in low-concentration. Herein, we propose a trypsin-activated catalysis reaction for amplified detection, which substantially improves the sensitivity of nanopore technique. The proposed trypsin-amplified nanopore amplified sandwich assay (tNASA) could contribute to ... More
A microparticle platform for STING-targeted immunotherapy enhances natural killer cell- and CD8
AuthorsWatkins-Schulz R, Tiet P, Gallovic MD, Junkins RD, Batty C, Bachelder EM, Ainslie KM, Ting JPY
JournalBiomaterials
PubMed ID30909112
Immunotherapies have significantly improved cancer patient survival, but response rates are still limited. Thus, novel formulations are needed to expand the breadth of immunotherapies. Pathogen associated molecular patterns (PAMPs) can be used to stimulate an immune response, but several pathogen recognition receptors are located within the cell, making delivery challenging. ... More
GLUT1 and GLUT3 involvement in anthocyanin gastric transport- Nanobased targeted approach.
AuthorsOliveira H, Roma-Rodrigues C, Santos A, Veigas B, Brás N, Faria A, Calhau C, de Freitas V, Baptista PV, Mateus N, Fernandes AR, Fernandes I
JournalSci Rep
PubMed ID30692585
Anthocyanins may protect against a myriad of human diseases. However few studies have been conducted to evaluate their bioavailability so their absorption mechanism remains unclear. This study aimed to evaluate the role of two glucose transporters (GLUT1 and GLUT3) in anthocyanins absorption in the human gastric epithelial cells (MKN-28) by ... More
Layer-by-layer assembled PLGA nanoparticles carrying miR-34a cargo inhibit the proliferation and cell cycle progression of triple-negative breast cancer cells.
AuthorsKapadia CH, Ioele SA, Day ES
JournalJ Biomed Mater Res A
PubMed ID31742868
Triple-negative breast cancer (TNBC) accounts for 15-25% of diagnosed breast cancers, and its lack of a clinically defined therapeutic target has caused patients to suffer from earlier relapse and higher mortality rates than patients with other breast cancer subtypes. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of ... More