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引用和文献 (17)
Invitrogen™
PowerLoad™ 浓缩液，100X
PowerLoad™ 是一种优化的非离子 Pluronic™ 表面活性剂聚醇配方，适用于在生理培养基中溶解水溶性染料和其他材料。这些表面活性剂（如 Pluronic™ F-127），已被用来帮助分散荧光离子指示剂的乙酰氧基甲 (AM)了解更多信息
| 货号 | 数量 |
|---|---|
| P10020 | 5 mL |
货号 P10020
价格（CNY)
2,770.00
Each
数量:
5 mL
价格（CNY)
2,770.00
Each
PowerLoad™ 是一种优化的非离子 Pluronic™ 表面活性剂聚醇配方，适用于在生理培养基中溶解水溶性染料和其他材料。这些表面活性剂（如 Pluronic™ F-127），已被用来帮助分散荧光离子指示剂的乙酰氧基甲 (AM) 酯，如 fluo-4、fura-2、indo-1、fluo-3 和 SBFI；它们似乎是装载其他染料所必需的（例如 SBFI-AM 或 PBFI-AM）。可选择将 PowerLoad™ 用于红色漂移钙指示剂和其他大分子量 AM 酯染料，并且也可将其用于分散其他亲脂性探针。PowerLoad™ 中 Pluronic™ 表面活性剂的浓度低于 0.2 %。PowerLoad™ 与水溶性 Probenecid (P36400) 配合使用以便在通过阴离子泵主动挤出去乙酰化形式的细胞中帮助上样和保留 AM 酯染料。总之，在成像和高通量筛选 (HTS) 应用中，这些试剂在装载染料时事半功倍。应采取适当控制以确保 PowerLoad™ 不会改变细胞的膜属性。
了解有关钙离子、钾离子、pH 和膜电位指示剂等离子指示剂更多信息›
了解有关钙离子、钾离子、pH 和膜电位指示剂等离子指示剂更多信息›
仅供科研使用。不可用于诊断程序。
规格
建议的储存条件储存在冰箱 (2–8°C) 中。
数量5 mL
Unit SizeEach
常见问题解答 (FAQ)
Can the Fluo-4 Calcium Imaging Kit (Cat. No. F10489) components be purchased separately?
引用和文献 (17)
引用和文献
Abstract
The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
Journal:J Biol Chem
PubMed ID:19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S.,
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
Journal:J Biol Chem
PubMed ID:22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to
Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss.
Journal:J Biol Chem
PubMed ID:21135109
'Our recent animal and human studies revealed that chronic hyponatremia is a previously unrecognized cause of osteoporosis that is associated with increased osteoclast numbers in a rat model of the human disease of the syndrome of inappropriate antidiuretic hormone secretion (SIADH). We used cellular and molecular approaches to demonstrate that
Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.
Journal:Sci Signal
PubMed ID:22472649
Metabotropic glutamate receptor (mGluR)-dependent calcium ion (Ca²+) signaling in astrocytic processes regulates synaptic transmission and local blood flow essential for brain function. However, because of difficulties in imaging astrocytic processes, the subcellular spatial organization of mGluR-dependent Ca²+ signaling is not well characterized and its regulatory mechanism remains unclear. Using genetically
Transient Ca2+ mobilization caused by osmotic shock initiates salmonid fish sperm motility.
Journal:J Exp Biol
PubMed ID:22279070
Salmonid fish sperm motility is known to be suppressed in millimolar concentrations of extracellular K(+), and dilution of K(+) upon spawning triggers cAMP-dependent signaling for motility initiation. In a previous study, however, we demonstrated that suspending sperm in a 10% glycerol solution and subsequent dilution into a low-osmotic solution induced