用于检测吞噬作用的 pHrodo™ Green E. coli BioParticles™ 偶联物

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用于检测吞噬作用的 pHrodo™ Green <i>E. coli</i> BioParticles™ 偶联物

引用和文献 (19)

Invitrogen™

用于检测吞噬作用的 pHrodo™ Green E. coli BioParticles™ 偶联物

使用用于检测吞噬作用的 pH 敏感性 pHrodo Green E. coli BioParticles 偶联物检测，实现更快的染色，得到更准确的结果，且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快了解更多信息

货号	数量
P35366	5 x 2 mg

货号 P35366

价格（CNY)

4,182.00

Ends: 31-Dec-2025

5,669.00

共减 1,487.00 (26%)

添加至购物车

5 x 2 mg

价格（CNY)

4,182.00

Ends: 31-Dec-2025

5,669.00

共减 1,487.00 (26%)

添加至购物车

使用用于检测吞噬作用的 pH 敏感性 pHrodo Green E. coli BioParticles 偶联物检测，实现更快的染色，得到更准确的结果，且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快、更精确的结果，可降低敏感实验中的信号变异性并改善时序。pHrodo Green 染料偶联物在细胞外无荧光，但在吞噬体中呈绿色强荧光，可专门用于检测细胞内吞噬和内吞作用。pHrodo Green E. coli BioParticles 偶联物也可与多种蓝色、红色和远红色染料报告基因（如 Mitosox Red、CellEvent Caspase 3/7 Red、NucBlue、RFP 和 Mitotracker Deep Red 等）配合使用进行多通路检测。

新型 pHrodo Green 染料的荧光随 pH 值从中性到酸性降低而显著增强，使其成为研究吞噬作用以及该作用受药物和/或环境因素调节情况的出色工具。缺乏细胞外荧光，无需清洗步骤和淬灭剂。

在成像、高内涵筛选、高通量筛选和流式应用中使用即用型 pHrodo Green E. coli BioParticles 偶联物。提供的试剂足够在 96 孔板的每个孔中使用 100µL 进行 100 次检测。提供的 pHrodo Green 染料也可作为 Zymosan BioParticles、S. aureus BioParticles 或葡聚糖 10,000 MW 的偶联物。如需制备其他偶联物，例如抗体偶联物，请使用 pHrodo Green STP 酯或 pHrodo Green 马来酰亚胺。

仅供科研使用。不可用于人或动物的治疗或诊断。

细胞类型哺乳动物

描述用于检测吞噬作用的 pHrodo™ Green E. coli BioParticles™ 偶联物

检测方法荧光

染料类型pHrodo™ Green

激发/发射509/533

数量5 x 2 mg

运输条件室温

种属大肠杆菌

适用于（应用）细胞分析

适用于（设备）Attune™ 声波聚焦流式细胞仪, 共聚焦显微镜, Floid™ 细胞成像系统, 荧光显微镜, 高内涵分析仪

产品线pHrodo

产品类型共轭物

Unit SizeEach

内容与储存

在 –20°C 干燥条件下避光储存。

常见问题解答 (FAQ)

I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?

pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I store reconstituted pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry?

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How many bioparticles are provided in pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35366)?

pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35366) contains ~3 x 10⁸ bioparticles/mg bioparticles before conjugation. We do not count the number of bioparticles after conjugation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all 用于检测吞噬作用的 pHrodo™ Green E. coli BioParticles™ 偶联物 FAQs

引用和文献 (19)

引用和文献

Abstract

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.

Authors:Dolman NJ, Kilgore JA, Davidson MW,

Journal:

PubMed ID:23835803

'Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of ... More

TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis.

Authors:Kleinberger G, Yamanishi Y, Suárez-Calvet M, Czirr E, Lohmann E, Cuyvers E, Struyfs H, Pettkus N, Wenninger-Weinzierl A, Mazaheri F, Tahirovic S, Lleó A, Alcolea D, Fortea J, Willem M, Lammich S, Molinuevo JL, Sánchez-Valle R, Antonell A, Ramirez A, Heneka MT, Sleegers K, van der Zee J, Martin JJ, Engelborghs S, Demirtas-Tatlidede A, Zetterberg H, Van Broeckhoven C, Gurvit H, Wyss-Coray T, Hardy J, Colonna M, Haass C,

Journal:

PubMed ID:24990881

'Genetic variants in the triggering receptor expressed on myeloid cells 2 (TREM2) have been linked to Nasu-Hakola disease, Alzheimer''s disease (AD), Parkinson''s disease, amyotrophic lateral sclerosis, frontotemporal dementia (FTD), and FTD-like syndrome without bone involvement. TREM2 is an innate immune receptor preferentially expressed by microglia and is involved in inflammation ... More

P2X4 receptor regulates alcohol-induced responses in microglia.

Authors:Gofman L, Cenna JM, Potula R

Journal:

PubMed ID:25135400

'Mounting evidence indicates that alcohol-induced neuropathology may result from multicellular responses in which microglia cells play a prominent role. Purinergic receptor signaling plays a key role in regulating microglial function and, more importantly, mediates alcohol-induced effects. Our findings demonstrate that alcohol increases expression of P2X4 receptor (P2X4R), which alters the ... More

Induction of trabecular meshwork cells from induced pluripotent stem cells.

Authors:Ding QJ, Zhu W, Cook AC, Anfinson KR, Tucker BA, Kuehn MH

Journal:

PubMed ID:25298418

Loss or dysfunction of trabecular meshwork (TM) cells has been associated with the development of pathologically elevated IOP, and it is conceivable that replacement of damaged TM cells could restore function to the TM. We propose that the use of TM-like cells derived from induced pluripotent stem cells (iPSCs) created ... More

Pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.

Authors:Matsui A, Jin JO, Johnston CD, Yamazaki H, Houri-Haddad Y, Rittling SR,

Journal:

PubMed ID:25024367

Endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. To investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections ... More

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