Search
Search
View additional product information for pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis - FAQs (P35366)
6 product FAQs found
pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
pHrodo Green E. coli BioParticles Conjugate for Phagocytosis (Cat. No. P35366) contains ~3 x 108 bioparticles/mg bioparticles before conjugation. We do not count the number of bioparticles after conjugation.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.
We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.