pHrodo™ Green STP 酯

New, second-generation pHrodo dyes are more soluble and reactive - ideal for antibody labeling.

引用和文献 (9)

Invitrogen™

pHrodo™ Green STP 酯

具有胺反应性和 pH 值敏感性的 pHrodo™ Green STP 酯染料，适用于生成生物偶联物以研究内吞作用和吞噬作用。pHrodo™ Green 随着其周围环境的了解更多信息

货号	数量
P35369	500 µg

货号 P35369

价格（CNY)

7,034.00

添加至购物车

500 µg

价格（CNY)

7,034.00

添加至购物车

具有胺反应性和 pH 值敏感性的 pHrodo™ Green STP 酯染料，适用于生成生物偶联物以研究内吞作用和吞噬作用。pHrodo™ Green 随着其周围环境的 pH 值酸性增强而显著增强荧光。
•利用 pH 值敏感性 pHrodo™ Green STP 酯生成您选择的 pH 值敏感性生物偶联物
• 与任何其他内吞作用或吞噬作用检测相比，获得更快、更准确的结果—不需要洗涤步骤或淬灭剂
• 可与红色荧光标记物（如 RFP、pHrodo™ Red 和多种其他标记物）进行多重分析

随 pH 值从碱性变化至酸性时 pHrodo™ Green 荧光的增加与细胞内囊泡酸化相关联，使其成为研究内吞作用或吞噬作用以及通过环境因素、药物或病原体调控内吞作用或吞噬作用的一种理想工具。pHrodo™ Green 的光谱特性使其可用于多色实验。pHrodo™ Green 可使用最标准绿色荧光滤光片组进行检测，并经验证可用于各种平台，包括流式细胞分析、荧光显微镜检测以及高内涵筛选 (HCS)。pHrodo™ Green 在典型细胞外环境中无荧光而无需在实验工作流程中进行洗涤步骤或淬灭染料。

pHrodo™ Green STP 酯是一种可在缓冲溶液中进行 pHrodo™ Green 生物偶联的胺反应性染料。STP 酯可与蛋白、细胞或病毒上的伯胺反应，从而生成可用于活细胞测定或储存后续使用的稳定偶联物。

胺反应性 pHrodo™ 染料同样适用于红色荧光测定（参见 pHrodo™ Red SE）。此外，硫醇反应性 pHrodo™ Red 马来酰亚胺和 Green 马来酰亚胺是用于抗体标记的胺反应性染料的重要替代物。硫醇标记确保染料不会附着于抗体的结合区域。pHrodo™ Green 和 Red 偶联物（例如葡聚糖、大肠杆菌和金黄色葡萄球菌）也以即用型形式提供。

仅供研究使用。不可用于人或动物的治疗或诊断。

仅供科研使用。不可用于诊断程序。

化学反应性胺

检测方法荧光

EC 消光系数75000

发射525

激发505

标签或染料pHrodo™ Green

产品类型STP 酯

数量500 µg

反应基团STP 酯

运输条件室温

溶解度DMSO（二甲亚砜）

产品线pHrodo

Unit SizeEach

内容与储存

1 小瓶，干燥、避光储存于 ≤ -20°C 下。

常见问题解答 (FAQ)

I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?

pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (9)

引用和文献

Abstract

The well-coordinated linkage between acidogenicity and aciduricity via insoluble glucans on the surface of Streptococcus mutans.

Authors:Guo L, McLean JS, Lux R, He X, Shi W,

Journal:

PubMed ID:26657939

Streptococcus mutans is considered the principal cariogenic bacterium for dental caries. Despite the recognition of their importance for cariogenesis, the possible coordination among S. mutans' main virulence factors, including glucan production, acidogenicity and aciduricity, has been less well studied. In the present study, using S. mutans strains with surface-displayed pH-sensitive ... More

Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancer.

Authors:Bosco EE, Christie RJ, Carrasco R, Sabol D, Zha J, DaCosta K, Brown L, Kennedy M, Meekin J, Phipps S, Ayriss J, Du Q, Bezabeh B, Chowdhury P, Breen S, Chen C, Reed M, Hinrichs M, Zhong H, Xiao Z, Dixit R, Herbst R, Tice DA,

Journal:Oncotarget

PubMed ID:29796165

'Despite recent advances in treatment, breast cancer remains the second-most common cause of cancer death among American women. A greater understanding of the molecular characteristics of breast tumors could ultimately lead to improved tumor-targeted treatment options, particularly for subsets of breast cancer patients with unmet needs. Using an unbiased genomics ... More

Genetically Engineered iPSC-Derived FTDP-17 MAPT Neurons Display Mutation-Specific Neurodegenerative and Neurodevelopmental Phenotypes.

Authors:Verheyen A, Diels A, Reumers J, Van Hoorde K, Van den Wyngaert I, van Outryve d'Ydewalle C, De Bondt A, Kuijlaars J, De Muynck L, De Hoogt R, Bretteville A, Jaensch S, Buist A, Cabrera-Socorro A, Wray S, Ebneth A, Roevens P, Royaux I, Peeters PJ

Journal:Stem Cell Reports

PubMed ID:30057263

'Tauopathies such as frontotemporal dementia (FTD) remain incurable to date, partially due to the lack of translational in vitro disease models. The MAPT gene, encoding the microtubule-associated protein tau, has been shown to play an important role in FTD pathogenesis. Therefore, we used zinc finger nucleases to introduce two MAPT mutations ... More

Dietary obesity reversibly induces synaptic stripping by microglia and impairs hippocampal plasticity.

Authors:Hao S, Dey A, Yu X, Stranahan AM,

Journal:Brain Behav Immun

PubMed ID:26336035

'Obesity increases risk of age-related cognitive decline and is accompanied by peripheral inflammation. Studies in rodent models of obesity have demonstrated that impaired hippocampal function correlates with microglial activation, but the possibility that neuron/microglia interactions might be perturbed in obesity has never been directly examined. The goal of this study ... More

Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens.

Authors:Haney MS, Bohlen CJ, Morgens DW, Ousey JA, Barkal AA, Tsui CK, Ego BK, Levin R, Kamber RA, Collins H, Tucker A, Li A, Vorselen D, Labitigan L, Crane E, Boyle E, Jiang L, Chan J, Rincón E, Greenleaf WJ, Li B, Snyder MP, Weissman IL, Theriot JA, Collins SR, Barres BA, Bassik MC,

Journal:Nat Genet

PubMed ID:30397336

Phagocytosis is required for a broad range of physiological functions, from pathogen defense to tissue homeostasis, but the mechanisms required for phagocytosis of diverse substrates remain incompletely understood. Here, we developed a rapid magnet-based phenotypic screening strategy, and performed eight genome-wide CRISPR screens in human cells to identify genes regulating ... More

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