Degradation of heparan sulfate proteoglycans enhances oxidized-LDL-mediated autophagy and apoptosis in human endothelial cells.
AuthorsDing Z, Wang X, Khaidakov M, Liu S, Dai Y, Mehta JL,
JournalBiochem Biophys Res Commun
PubMed ID22910414
Cell surface heparan sulfate proteoglycans (HSPG) play an important role in atherogenesis. We hypothesized that degradation of HSPG may increase the binding of atherogenic oxidized low density lipoprotein (ox-LDL) to endothelial cells, and result in extensive HSPG degradation as well as autophagy and apoptosis. Primary human umbilical vein endothelial cells ... More
Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity.
AuthorsDash R, Azab B, Quinn BA, Shen X, Wang XY, Das SK, Rahmani M, Wei J, Hedvat M, Dent P, Dmitriev IP, Curiel DT, Grant S, Wu B, Stebbins JL, Pellecchia M, Reed JC, Sarkar D, Fisher PB,
JournalProc Natl Acad Sci U S A
PubMed ID21555592
'Limited options are available for treating patients with advanced prostate cancer (PC). Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24), an IL-10 family cytokine, exhibits pleiotropic anticancer activities without adversely affecting normal cells. We previously demonstrated that suppression of the prosurvival Bcl-2 family member, myeloid cell leukemia-1 (Mcl-1), is required for mda-7/IL-24-mediated apoptosis ... More
Combining imaging and pathway profiling: an alternative approach to cancer drug discovery.
AuthorsCarragher NO, Brunton VG, Frame MC,
JournalDrug Discov Today
PubMed ID22493783
Conventional drug discovery strategies are typically 'target centric' based on the selection of lead compounds with optimised 'on-target' potency and selectivity profiles. However, high-attrition rates are often the result of compensatory or redundant cancer mechanisms and the fact that tumours do not find it difficult to escape inhibition of a ... More
Long-term effectiveness of the Burch colposuspension in female urinary stress incontinence.
Of 91 women who underwent Burch colposuspension, 86 were available for clinical, urodynamic and isotope renographic follow-up examinations 5 years after surgery for evaluation of late complications and long-term effectiveness in urinary stress incontinence. A repeat urodynamic examination was accepted by 76 patients. Stress incontinence was cured in 71% of ... More
Arrayed CRISPR reveals genetic regulators of tau aggregation, autophagy and mitochondria in Alzheimer's disease model.
Authors
JournalSci Rep
PubMed ID33536571
Facile size-controlled synthesis of fucoidan-coated gold nanoparticles and cooperative anticancer effect with doxorubicin.
AuthorsJang H, Kang K, El-Sayed MA
JournalJ Mater Chem B
PubMed ID32264368
'To conquer cancer, one of the most dangerous and common diseases faced by humanity, many therapeutic approaches have been researched and developed. Discovery of highly effective therapeutic molecules without side effects and novel strategies for their effective delivery are areas receiving recent global interest. Here, we describe a facile one-pot ... More
Lymphatic endothelial cells are a replicative niche for Mycobacterium tuberculosis.
Authors
JournalJ Clin Invest
PubMed ID26901813
Live Cell Imaging of Enzymatic Turnover of an Adenosine 5'-Tetraphosphate Analog.
Authors
JournalInt J Mol Sci
PubMed ID34445322
Non-linear optical imaging of atherosclerotic plaques in the context of SIV and HIV infection prominently detects crystalline cholesterol esters.
Authors
JournalPLoS One
PubMed ID33984028
HIF-1α-BNIP3-mediated mitophagy in tubular cells protects against renal ischemia/reperfusion injury.
Authors
JournalRedox Biol
PubMed ID32829253
Combining protein and mRNA quantification to decipher transcriptional regulation.
Authors
JournalNat Methods
PubMed ID26098021
A novel image-based cytometry method for autophagy detection in living cells.
Authors
JournalAutophagy
PubMed ID22895056
3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy.
Authors
JournalJ Cell Sci
PubMed ID27445312
BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes.
Authors
JournalAutophagy
PubMed ID33404293
A broad-spectrum antibiotic, DCAP, reduces uropathogenic Escherichia coli infection and enhances vorinostat anticancer activity by modulating autophagy.
Authors
JournalCell Death Dis
PubMed ID30006504
Impaired mitophagy activates mtROS/HIF-1a interplay and increases cancer aggressiveness in gastric cancer cells under hypoxia.
AuthorsShida M, Kitajima Y, Nakamura J, Yanagihara K, Baba K, Wakiyama K, Noshiro H
JournalInt J Oncol
PubMed ID26820502
Mitochondrial autophagy (mitophagy) is a selective form of autophagy and a critical step in excluding mitochondria damaged by stress, including hypoxia. This study aimed to determine whether the integrity of mitophagy affected production of the mitochondrial reactive oxygen species (mtROS), hypoxia inducible factor (HIF)-1a expression and aggressive characteristics in GC ... More
Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles.
AuthorsJang H, Kang K, El-Sayed MA
JournalJ Mater Chem B
PubMed ID32254605
To date, a variety of biological assays such as immunostaining, western blotting, enzyme-linked immunosorbent assay (ELISA), and flow cytometry have been used to analyze and trace important biological events and therapies. In addition to these techniques, the application of microscopic analytical techniques such as matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) ... More
Cell membrane disintegration and extracellular vesicle release in a model of different size and charge PAMAM dendrimers effects on cultured endothelial cells.
AuthorsPatel M, De Paoli SH, Elhelu OK, Farooq S, Simak J
JournalNanotoxicology
PubMed ID30729845
Different nanomaterials are under development for various biomedical applications in which nanoparticles contact blood and vasculature. Therefore, investigating the interactions between nanomaterials and vascular endothelial cells (ECs) is of great importance. Here, we show the effects of polyamidoamine (PAMAM) dendrimers of two different sizes, generation 2 (G2; approximately 3 ... More