水溶性丙磺舒
水溶性丙磺舒
Invitrogen™

水溶性丙磺舒

丙磺舒通常用于抑制细胞膜中的有机阴离子转运蛋白。这些转运蛋白可以将染料和指示剂排出细胞，在利用细胞内保留的染料或指示剂进行的检测中，这会导致加载不良或背景信号偏高。Di Virgilio 等人 (1990) 较早描述了丙磺舒在封闭细胞内染料外排方面的应用，其已与多种阴离子染料和偶联物配合使用。常用的游离酸形式的丙磺舒很难溶解，需要 1 M了解更多信息
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货号数量
P3640010 x 77 mg
货号 P36400
价格（CNY)
4,688.00
Each
添加至购物车
数量:
10 x 77 mg
价格（CNY)
4,688.00
Each
添加至购物车
丙磺舒通常用于抑制细胞膜中的有机阴离子转运蛋白。这些转运蛋白可以将染料和指示剂排出细胞，在利用细胞内保留的染料或指示剂进行的检测中，这会导致加载不良或背景信号偏高。Di Virgilio 等人 (1990) 较早描述了丙磺舒在封闭细胞内染料外排方面的应用，其已与多种阴离子染料和偶联物配合使用。常用的游离酸形式的丙磺舒很难溶解，需要 1 M NaOH 才能使其溶解。我们的水溶性丙磺舒 (P36400) 可快速在检测缓冲液中溶解，而且无需使用腐蚀性的 NaOH。水溶性丙磺舒包含在 Fluo-4 NW 钙含量检测试剂盒（F36205、F36206）中，也可单独提供。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

仅供科研使用。不可用于诊断程序。
规格
数量10 x 77 mg
建议的储存条件室温保存
运输条件室温
物理形态实心
产品类型细胞标记试剂
Unit SizeEach

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.