The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
AuthorsGeng Y, Hoke A, Delpire E,
JournalJ Biol Chem
PubMed ID19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S., ... More
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
AuthorsClementi EA, Marks LR, Duffey ME, Hakansson AP,
JournalJ Biol Chem
PubMed ID22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to ... More
Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers.
Fura-2 is widely used to measure the concentration of cytosolic free calcium, but in many cells the dye does not remain localized within the cytoplasmic matrix. In these cells, Fura-2 is sequestered within intracellular organelles, secreted into the extracellular medium, or both. We have found that, in mouse peritoneal macrophages, ... More
Genetic divergence of rotavirus nonstructural protein 4 results in distinct serogroup-specific viroporin activity and intracellular punctate structure morphologies.
AuthorsHyser JM, Utama B, Crawford SE, Estes MK,
JournalJ Virol
PubMed ID22357281
Nonstructural protein 4 (NSP4) viroporin activity is critical for the replication and assembly of serogroup A rotavirus (RVA); however, the dramatic primary sequence divergence of NSP4s across serogroups raises the possibility that viroporin activity is not a common feature among RVs. We tested for NSP4 viroporin activity from divergent strains, ... More
Cell-based potassium ion channel screening using the FluxOR assay.
AuthorsBeacham DW, Blackmer T, O' Grady M, Hanson GT,
JournalJ Biomol Screen
PubMed ID20208034
FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye. This indicator reports channel activity with a ... More
ATP-induced P2X7-associated uptake of large molecules involves distinct mechanisms for cations and anions in macrophages.
AuthorsSchachter J, Motta AP, de Souza Zamorano A, da Silva-Souza HA, Guimarães MZ, Persechini PM,
JournalJ Cell Sci
PubMed ID18782864
Macrophages express the P2X(7) receptor and other nucleotide (P2) receptors, and display the phenomenon of extracellular ATP (ATP(e))-induced P2X(7)-dependent membrane permeabilization, which occurs through a poorly understood mechanism. We used patch-clamp recordings, cytoplasmic Ca(2+) measurements and fluorescent dye uptake assays to compare P2X(7)-associated transport phenomena of macrophages and HEK-293 cells ... More
Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions.
Fura-2, loaded into J774.2 macrophages as the acetoxymethyl ester, is sequestered into intracellular vacuoles within 90 min after the beginning of the loading at 37 degrees C. The dye is also efficiently secreted from the cells. Sequestration and secretion of fura-2 reduce the accuracy of measurements of cytosolic free Ca2+ ... More
Fluorescence imaging study of organic anion transport from renal proximal tubule cell to lumen.
AuthorsMiller DS, Letcher S, Barnes DM
JournalAm J Physiol
PubMed ID8853412
The mechanisms driving organic anion transport from cell to lumen were studied in intact killifish proximal tubules using fluorescence microscopy. Three fluorescent substrates were used as follows: 1) fluorescein (FL); 2) carboxyfluorescein (CF), generated intracellularly from carboxyfluorescein diacetate (CFDA); and 3) bimane-S conjugates, generated intracellularly by conjugation of monochlorobimane (MCB) ... More
Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited.
AuthorsBallatori N, Hager DN, Nundy S, Miller DS, Boyer JL
JournalAm J Physiol
PubMed ID10516157
Uptake of lucifer yellow (LY), a fluorescent disulfonic acid anionic dye, was studied in isolated skate (Raja erinacea) perfused livers and primary hepatocytes to evaluate its utility as a fluid-phase marker in these cells. However, our findings demonstrated that LY is transported across the plasma membrane of skate hepatocytes largely ... More
Pharmacokinetics of 5 (and 6)-carboxy-2',7'-dichlorofluorescein and its diacetate promoiety in the liver.
Hepatic disposition of 5 (and 6)-carboxy-2',7'-dichlorofluorescein (CDF) and its diacetate promoiety (CDFDA) was studied in isolated perfused rat livers. Livers from Wistar wild-type and multidrug resistance-associated protein (Mrp)2-deficient (TR(-)) rats were perfused with CDF in the presence or absence of probenecid. Probenecid decreased the recovery of CDF in bile approximately ... More
Accumulation and extrusion of permeant Ca2+ chelators in attenuation of synaptic transmission at hippocampal CA1 neurons.
AuthorsOuanounou A, Zhang L, Tymianski M, Charlton MP, Wallace MC, Carlen PL
JournalNeuroscience
PubMed ID8923526
The effects of extracellularly applied membrane-permeant Ca2+ chelators on field excitatory postsynaptic potentials were determined in the hippocampal CA1 region of rat brain slices. Field excitatory postsynaptic potentials in slices perfused with 0.05-50 microM bis-(-O-aminophenoxy)-ethane-N,N,N,N,-tetraacetic acid acetoxymethyl (BAPTA-AM) for 15 min were reversibly attenuated by 10-45% in a concentration-dependent manner. ... More
Novel metabolic role for BDNF in pancreatic β-cell insulin secretion.
Authors
JournalNat Commun
PubMed ID32327658
Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung.
Authors
JournalNat Commun
PubMed ID31481690
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium.
Authors
JournalElife
PubMed ID32579112
Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531.
Authors
JournalElife
PubMed ID32667883
The furan fatty acid metabolite CMPF is elevated in diabetes and induces β cell dysfunction.
Authors
JournalCell Metab
PubMed ID24703697
Optogenetic activation of spinal microglia triggers chronic pain in mice.
Authors
JournalPLoS Biol
PubMed ID33739978
Homozygous ALS-linked FUS P525L mutations cell- autonomously perturb transcriptome profile and chemoreceptor signaling in human iPSC microglia.
Authors
JournalStem Cell Reports
PubMed ID35120624
Farnesyl pyrophosphate is a new danger signal inducing acute cell death.
Authors
JournalPLoS Biol
PubMed ID33901180
Discovery of Human Signaling Systems: Pairing Peptides to G Protein-Coupled Receptors.
Authors
JournalCell
PubMed ID31675498
Neuronal hyperexcitability drives central and peripheral nervous system tumor progression in models of neurofibromatosis-1.